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Prevention of La^(3+) on DNA Damage Caused by Hg^(2+) from Fish Intestines

Journal of Rare Earths 2007年第2期25卷 243-248页

作者：洪法水王玲刘超苏明玉黄浩陈亮 College of Life Sciences Library Suzhou University

The aim of this study was to investigate the effects of rare earth elements （REEs） in preventing Hg^2＋ pollution, using fish intestinal DNA in vitro and study the mechanism of the interactions between Hg^2＋ , La^3＋ , the mixture of La^3＋ and Hg^2＋ and DNA by spectroscopy. The interactions between Hg^2＋ , La^3＋ , the mixture of La^3＋ and Hg^2＋ and DNA from fish intestine in vitro was investigate by using absorption spectrum and fluorescence emission spectrum. Ultraviolet absorption spectra indicated that the addition of Hg^2＋ , La^3＋ , and the mixture of La^3＋ and Hg^2＋ to DNA generated obvious hypochromic effect. Meanwhile, the 205.2 nm peak of DNA blue and the 258.2 nm peak of DNA red shifted. The hypochromic effect and peak shift was caused by these ions in an order of Hg^2＋〉 Hg^2＋＋ La^3＋〉 La^3＋ . The fluorescence emission spectra showed that as the addition of Hg^2＋ , La^3＋ , and the mixture of La^3＋ and Hg^2＋ , the emission peak at about 416.2 nm of DNA did not obviously change, but the fluorescence intensity reduced gradually with the order in treatment was Hg^2＋〉 Hg^2＋〉 La^3＋〉 La^3＋ . Hg^2＋ , La^3＋ , and the mixture of La^3＋ and Hg^2＋ had 1.12, 0.58, and 0.81 binding sites to DNA, the fluorescence quenching of DNA caused by them all attributed to static quenching. The binding constants KA of binding sites were 3.82×10^4 and 4.22×10^2 L·mol^-1 ; 2.50×10^4 and 2.95×10^3 L·mol^-1 ; 3.05×10^4 and 1.00×10^3 L·mol^-1. The results showed that La^3＋ could relieve destruction caused by Hg^2＋ on the DNA structure.

关键词： Hg^2+ , La^3+ fish intestinal DNA spectral characterization binding constants rare earths

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Synthesis, DNA and Photocleavage Studies of Ru（ll） Polypyridyl Complexes： [Ru（dppz）（pyz）4]（ClO4）2 and [Ru（dppz）（dmpyz）4]（ClO4）2 Complexes

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Chinese Journal of Chemistry 2012年第7期30卷 1641-1646页

作者： Pallavi,Ponuganti Nagababu,Penumaka Laxmareddy,Kotha Padmaja,Naishadham Satyanarayana,Sirasani Department of Chemistry Osmania UniversityHyderabad-500007Andhra PradeshIndia

In view of the growing interest for the synthesis of metal complexes and their interaction with DNA, we have synthesized and characterized two complexes containing ruthenium as metal center. The complexes are of the type [Ru（dppz）L4]（C104）2 where L are biologically important ligands such as pyrazole and dimethylpyrazole. The characterization of these complexes is done by 1 H NMR, 13C NMR, elemental analysis and mass spectroscopy. The interaction of these complexes with CT DNA was monitored and binding constants were determined using absorption and fluorescence spectroscopy. The mode of binding was found to be intercalative for both complexes and was determined using hydrodynamic viscosity studies. The complexes were further studied for photocleavage studies with supercoiled plasmid pBR322 DNA.

关键词： Ru(II) complexes fluorescence binding constants hydrodynamic viscosity supercoiled plasmid DNA pBR322

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Study of interaction between metal ions and quercetin

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Food Science and Human Wellness 2018年第3期7卷 215-219页

作者： Taiane Souza de Castilho Tatiane Brescovites Matias Keller Paulo Nicolini Jaqueline Nicolini Instituto Federal do Parana-IFPR Lacoppi-Laboratorio de Corantes e Processos PiroliticosDepartamento de QuimicaPalmasPRBrazil

A displacement test based on the interaction between the flavonoid quercetin and an excess of metal chloride allows the determination of the binding constant for the reaction between quercetin and Ca2+,Mg^2+and Ni^2+.The values obtained were 2.20±1.77×10^3 for Ca^2+,1.37±0.59×10^3 for Mg^2+and 7.03±1.04×10^4 for Ni^2+,and all interactions showed type 1:1 stoichiometry,as determined by titration and by the method of continuous variations(Job’s method).The complexion effect was observed qualitatively through a colorimetric change in the medium(yellow→neon yellow)and spectroscopically through a bathochromic shift in the absorption band of quercetin in the presence of *** investigation serves as a tool for the development and testing of materials capable of capturing toxic metal ions or favoring the absorption of beneficial ions(in relation to the human metabolism)through the construction of efficient bioorganic *** results reported herein allow understanding of this detection system,indicating the following ascending order of the binding constants(Mg^2+

关键词： binding constants Quercetin Antioxidant properties

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Investigation of the interaction between indigotin and two serum albumins by spectroscopic approaches

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Journal of Pharmaceutical Analysis 2013年第4期3卷 257-269页

作者： Zheng-Jun Cheng Hong-Mei Zhao Qian-Yong Xu Rong Liu Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province China West Normal University Nanchong Petrochemical School

The binding characteristics of indigotin with human serum albumin （HSA） and bovine serum albumin （BSA） have been investigated by various spectroscopic techniques. Spectroscopic analysis revealed that the quenching mechanism between indigotin and HSA/BSA belonged to the static quenching. The displacement experiments suggested that indigotin primarily bound to tryptophan residues on proteins within site I. The thermodynamic parameters indicated that the binding of indigoti^HSA/BSA mainly depended on the hydrophobic interaction. The binding distance of indigotin to HSA/BSA was evaluated. The results by synchronous fluorescence, three- dimensional fluorescence, Fourier Transform Infrared spectroscopy （FT-IR） and circular dichroism （CD） spectra showed that the conformation of proteins altered in the presence of indigotin.

关键词： Human serm albumin Bovine serum albumin Indigoiin Fluorescence spectro-scopy binding constants

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