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World Journal of Gastroenterology 2017年第26期23卷 4744-4751页

作者： Xia Yang Xian-Chun Gao Jun Liu Hong-Yu Ren Division of Gastroenterology Union Hospital Tongji Medical College Huazhong University of Science and Technology

AIM To investigate toll-like receptor 2(TLR2) and TLR4 expression, following bifidobacteria and low-dose EPEC endotoxin treatment, and intestinal barrier function in rat intestinal epithelial cell-18(IEC-18).METHODS Six experimental groups were established-normal control, EPEC, bifidobacteria infantis(B. infantis), B. longum, B. bifidum, and B. youth groups. Optimal EPEC endotoxin concentration, bifidobacteria fold dilution, and treatment duration were determined. Quantitative real-time polymerase chain reaction and western blot, respectively, were conducted to detect TLR2 and TLR4 m RNA and protein expression in IEC-18 cells. Transepithelial electrical resistance(TEER) was measured by the EVOM chopstick voltohmmeter in each group. All experiments were conducted in triplicate and data were analyzed on SPSS 16.RESULTS TLR2 and TLR4 m RNA and protein expression in the EPEC group were significantly higher than in the control group(P bifidobacteria protect IEC-18 cells against injury by down-regulating TLR2 and TLR4 expression and enhance intestinal barrier function to protect the intestinal epithelial cells from pathogenic invasion.

关键词： bifidobacteria Intestinal barrier function Intestinal epithelial cells

Research progress in the use of bifidobacteria for the treatment of colorectal cancer

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Gastroenterology & Hepatology Research 2022年第3期4卷 30-33页

作者： Yu-Ting Zhao Zhi-Hui Cai Yu-Chen Yang Graduate School Inner Mongolia Medical UniversityHohhot 010017China Department of Medical Oncology Inner Mongolia People’s HospitalHohhot 010017China

Colorectal cancer has high morbidity and mortality rates;therefore,developing new therapeutic drugs and methods for it is crucial.There is increasing evidence that intestinal flora plays a vital role in maintaining intestinal homeostasis and therefore,in the development of colorectal cancer.As such,therapeutic approaches using probiotics such as Bifidobacterium lactis to regulate intestinal flora are expected to represent a new treatment strategy for colorectal cancer.This article introduces and compares the development status of conventional therapies for colorectal cancer,primarily targeted therapy and immunotherapy,the relationship between intestinal flora and colorectal cancer,and the research and application of Bifidobacterium bifidum in colorectal cancer treatment to provide a reference for its clinical application.

关键词： colorectal cancer treatment intestinal flora bifidobacteria

SIGNAL MECHANISM OF INHIBITION OF bifidobacteria ON GROWTH OF COLON CANCER

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Chinese Journal of Cancer Research 2005年第2期17卷 145-149页

作者：戴建宜王立生朱惠明潘令嘉马晓东张亚历周殿元 Department of Gastroenterology Shenzhen Municipal People’s Hospital Shenzhen 518020 Department of Gastroenterology Nanfang Hospital Guangzhou 510515

Objective: To explore the antitumor mechanisms of bifidobacteria adolescence in vivo. Methods: The content of extracellular signal regulated proteins (ERK)1/2, C-Jun N-terminal kinase (JNK), p38, c-fos and c-jun in nude mouse transplanted large bowel carcinoma was detected by using laser confocal microscopy. The expression of NF-κB was determined by immunohistochemistry. Results: After the nude mouse transplanted tumor was treated with bifidobacteria, the average fluorescent strength of ERK1/2, JNK, c-fos and c-jun was significantly lower than that in tumor control group (P0.05). The positive cell density of NF-κB in large bowel carcinoma transplantation tumors in Bifidobacterium injection group was markedly lower than that in tumor group(Pbifidobacteria adolescence could markedly decrease the activity of ERK1/2 and JNK, the expression c-fos and c-jun, and the activity of NF-κB.

关键词： bifidobacteria Colon cancer Signal transduction C-jun N-terminal kinase Extracellular signal- regulated protein kinase NF-κB

bifidobacteria as Potential Functional Starter Cultures: A Case Study by MSc Students in Food Science and Technology (University of Foggia, Southern Italy)

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Food and Nutrition Sciences 2012年第1期3卷 55-63页

作者： Antonio Bevilacqua Maria Teresa Cagnazzo Cataldo Caldarola Emanuela Ciuffreda Anna Rita Dragano Stefania Franchino Rossana Lauriola Angela Pacifico Maria Rosaria Corbo Milena Sinigaglia 不详

This research paper was the results of activity of MSc students of Food Science and Technology, attending the class “Biotechnology of Functional Starter”. Five strains of bifidobacteria (Bifidobacterium animalis subsp. lactis;B. longum subsp. infantis;B. breve;B. animalis subsp. animalis;B. bifidum) were evaluated in order to assess their suitability as functional starter cultures, by studying the following technological and probiotic traits: growth at different temperatures, NaCl amounts and pH values;acidifying ability;metabolism (arginin deamination, esculin hydrolysis, acetoin production);survival at low pH and in presence of bile salts;hydrophobic properties;antibiotic resistance. After laboratory assays and strain selection through a multivariate analyses, it was highlighted that B. longum subsp. infantis and B. animalis subsp. lactis represent a good compromise as potential functional starter cultures, as B. animalis subp. lactis showed a high growth index at pH 5 and good values at 25?C and 30?C, as well as the minimal viability loss at pH 2.5. B. longum subsp. infantis DSMZ 20088 was the best microorganism for its growth index in presence of 6.5% of salt added and at 25?C and 30?C.

关键词： bifidobacteria Functional Starter Probiotic Properties Technological Characterization

bifidobacteria Upregulate Expression of Toll-Like Receptor Negative Regulators Counteracting Enterotoxigenic <i>Escherichia coli</i>Mediated Inflammation in Bovine Intestinal Epitheliocytes

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Open Journal of Veterinary Medicine 2013年第2期3卷 143-155页

作者： Kozue Murata Julio Villena Yohsuke Tomosada Risa Hara Eriko Chiba Tomoyuki Shimazu Hisashi Aso Yoshihito Suda Noriyuki Iwabuchi Jin-Zhong Xiao Tadao Saito Haruki Kitazawa Food and Feed Immunology Group Laboratory of Animal Products Chemistry Graduate School of Agricultural Science Tohoku University Sendai Japan

We previously established a bovine intestinal epithelial cell line (BIE cells) and showed that BIE cells are useful in vitro model system for the study of interactions between pathogenic and beneficial microorganisms and bovine intestinal epithelial cells (IECs). In the present study, we aimed to select potential immunomodulatory bifidobacteria that may be used to beneficially modulate the inflammatory response in bovine IECs. We also aimed to gain insight into the molecular mechanisms involved in the anti-inflammatory effect of bifidobacteria by evaluating the role of Toll-like receptor (TLR)-2 and TLR negative regulators in the regulation of proinflammatory cytokines production and MAPK, NF-κB and PI3K pathways activation in BIE cells. Five bifidobacteria strains were evaluated in this study and according to their capacity to modulate the inflammatory response of BIE cells. Despite the unique effect of each strain, four common points were found when comparing the effect of the high and moderate anti-inflammatory strains: 1) Upregulation of TLR negative regulators and the intensity of that upregulation was related to the different immunomodulatory capacity of each bifidobacteria strain;2) The balance between MAPK activation and MKP-1 upregulation affected the anti-inflammatory effect of bifidobacteria in BIE cells;3) The inhibition of PI3K pathway was related to the anti-inflammatory effect of bifidobacteria;4) The immunoregulatory effect of bifidobacteria in BIE cells is partially dependent on TLR2. This study shows that BIE cells can be used for the selection of immunoregulatory bifidobacteria and for studying the mechanisms involved in the protective activity of immunobiotics against TLR4-induced inflammatory damage. In addition, we have demonstrated that the anti-inflammatory effect of bifidobacteria was achieved by a complex interaction of multiple TLRs negative regulators as well as the inhibition/activation of multiple signaling pathways.

关键词： Bovine Intestinal Epithelial Cells bifidobacteria ETEC TLRs Negative Regulators

A preliminary clinical study of bifidobacteria preparation on the treatment of diarrhea in severely burned patients

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Chinese Medical Journal 1998年第4期 94-95页

作者：陈军张雅萍肖光夏

Objective To investigate the role of bifidobacteria preparation in the prevention of diarrhea in severely burned patients. Methods Forty three severely burned patients who were inflicted by diarrhea were included in this study. The changes of intestinal microflora were observed. Results The intestinal flora of all patients with diarrhea changed greatly before treatment. The proportions of bifidobacteria and bacteroid decreased significantly, whereas those of aerobe and Candida increased relatively. The ratio of anaerobe to aerobe decreased. As a result, the patients' intestinal flora were distorted. After six days treatment of bifidobacteria, diarrhea in most patients ceased and the intestinal microflora restored. Conclusion bifidobacteria feeding plays an important role in restoring intestinal microflora and stopping diarrhea in severely burned patients.

关键词： study clinical 陈军 bifidobacteria

Deep Exploration of bifidobacteria through Metabolomics Study

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Journal of Biosciences and Medicines 2018年第5期6卷 57-62页

作者： Juan Li Yatao Jiang Yihao Shen Qingzhi Li Zhongke Sun Institute of Food and Drug Inspection College of Life Sciences and Agronomy Zhoukou Normal University Zhoukou China Library of Zhoukou Normal University Zhoukou China

bifidobacteria are probiotic bacteria with multiple health-promoting properties for human being. The global market for probiotics, especially for bifidobacteria is booming. However, the entire market is still at an early stage as there is nearly no fine products developed yet except the whole bacterial cells. The maturation of metabolomics technologies make it possible to study complex mixture with high-throughput, comprehensive maps and libraries. Therefore, we prospect that metabolomics studies mainly based on liquid/gas chromatography-mass spectrometry (LC/GC-MS) can deepen our understanding in detail during the study of metabolic mechanisms of bifidobacteria. These studies can be conducted at three phases, including non-targeted, targeted metabolomic analysis of bifidobacteria, and specific metabolites production through metabolic engineering and fermentation. Metabolomic studies of bifidobacteria will allow us to fully explore their metabolic mechanisms and to utilize metabolites that contribute to human health. In particular, bifidobacteria derived conjugated linoleic acids and bacteriocins are two kinds of fined products that may have great potentials in the future and can be used as food additives.

关键词： bifidobacteria Metabolomics Metabolites Conjugated Linoleic Acid Probiotics Derived Fine Products

Bioinformatic Survey of S-Layer Proteins in bifidobacteria

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Computational Molecular Bioscience 2018年第2期8卷 68-79页

作者： Juan Li Yihao Shen Yatao Jiang Le He Zhongke Sun Institute of Food and Drug Inspection College of Life Sciences and Agronomy Zhoukou Normal University Zhoukou China Library of Zhoukou Normal University Zhoukou China

Surface layer (S-layer) proteins are one of the most commonly observed cell envelope components in both Archaea and Bacteria. It has versatile functions and holds considerable application potential in biotechnology. bifidobacteria are representative probiotics conferring health promoting properties. However, there is little study of S-layer in bifidobacteria yet. The distribution and characteristics of S-layer in bifidobacteria are unknown. In this study, search for S-layer protein in the identical protein groups in NCBI yielded 49 hits belonging to bifidobacteria. These proteins were annotated as either “S-layer (domain) protein” or “putative S-layer (y) domain protein” that distributed among 26 species of Bifidobacterium genus. Multiple alignments suggest S-layer proteins are relatively conservative. Phylogenetic analysis of 24 S-layer (domain) protein sequences groups them into three distinct clusters, with the majority species in Cluster-2. S-layer (domain) protein has a universe motif DUF4381, though its function is unknown. Meanwhile, two other motifs CARDB and EphA2_TM involved in cell adhesion and cell signaling respectively, presented in most S-layer (domain) protein in bifidobacteria. All S-layer proteins have a typical N-terminal Sec-dependent signal peptide and a C-terminal trans-membrane region. Homological modeling of representative S-layer proteins from each cluster revealed a few unique structural features. All representative S-layer proteins have a plenty of β-meander motif that exclusively composed by β-barrel structural architectures linked together by hairpin loops.

关键词： bifidobacteria S-layer Domain Protein Phylogeny Homology Modeling Domain

Functional Characterization of bifidobacteria of Human Origin: A Case Study by the Students of Food Science and Technology of the University of Foggia (Southern Italy)

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Food and Nutrition Sciences 2014年第13期5卷 1153-1161页

作者： Emanuela Ciuffreda Angino Veronica Antonietta Cifelli Rossana Foti Rosaria Ilenia Forte Fabio Graziani Andrea Giuseppe Longo Angela Longo Valeria Maglia Emilio Francesco Ricciardi Alessandra Sabatino Annamaria Tomaiuolo Maria Rosaria Corbo Milena Sinigaglia Antonio Bevilacqua Department of the Science of Agriculture Food and Environment (SAFE) University of Foggia Foggia Italy

The aim of this paper was to study the potential technological and probiotic properties of bifidobacteria isolated from human feces. bifidobacteria, naturally present in the dominant colonic microbiota, represent up to 25% of the cultivable faecal bacteria in adults and 80% in infants. bifidobacteria have been shown to adhere and colonize in high numbers different types of cultured intestinal epithelial cells;moreover some authors reported that some strains are able to stabilize the intestinal microbiota during and after antibiotic therapy, modulate the immune system, protecting against chemically induced intestinal inflammation and reducing symptoms of colitis. Eight isolates of bifidobacteria were studied to assess their technological and probiotic traits;the technological characterization relied on the assessment of enzymatic activities (proteolytic and lipolytic activity), growth under various conditions (pH, temperature and addition of salt), acidifying ability and metabolism (arginine deamination, esculin, esculin hydrolysis and citrate metabolism). The study of the probiotic characteristics focused on the evaluation of the survival at low pH and with bile salts added, antibiotic resistance, and hydrophobic properties. As a result of this process, two promising strains were selected for further studies.

关键词： bifidobacteria Technology Probiotic Traits Functional Characterization

Effects of Fructooligofructoses Chain Length on the bifidobacteria of the Human Colon: A Pilot Study

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Food and Nutrition Sciences 2012年第12期3卷 1615-1618页

作者： Krista Mendlik Julie A. Albrecht Marilynn Schnepf Department of Nutrition and Health Sciences University of Nebraska—Lincoln Lincoln USA

Human gastrointestinal health may be improved by the consumption of prebiotic food ingredients such as fructooligo-fructoses. A study was initiated to determine the effect of fructooligofructoses of different chain lengths on gastrointes-tinal parameters. Nineteen healthy subjects aged 20 - 57 y took part in a 10-week cross-over designed study. Subjects consumed either inulin or oligofructose for 3 weeks followed by a 2-week washout period between treatments. Stool samples were collected five times (baseline, 2 treatments, 2 washout) and analyzed for bifidobacteria. Daily records were kept for stool frequency, stool consistency and flatulence frequency. bifidobacteria counts (cfu/ml) were higher (trending toward significance) during inulin and oligofructose intakes (1.2 × 107 ± 4.8 × 107 and 2.0 × 108 ± 4.7 × 108) and washout periods (2.9 × 106 ± 6.5 × 106 and 1.1 × 107 ± 1.6 × 107) than baseline counts (2.2 × 105 ± 5.1 × 105 and 2.9 × 106 ± 6.5 × 106), respectively. Inulin and oligofructose treatment periods had a significant effect on stool consistency (watery/very hard) and flatulence frequency, but not stool frequency, when compared to baseline (P < 0.05). Further research is needed to confirm these results due to small sample size and the need for a longer washout period between treatments.

关键词： Prebiotics Fructooligofructoses Inulin Oligofructose bifidobacteria