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Recyclable thiol-modified Zr-based MOFs/hydrogel composite beads for effective removal of Hg(Ⅱ)

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Progress in Natural Science:Materials International 2023年第5期33卷 644-651页

作者： Xuechun Wang Zeming Dai Xuan Zhou Hao Chen Yu Cao Jianhua Hou Xiaozhi Wang College of Environmental Science and Engineering Yangzhou University Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Institutes of Agricultural Science and Technology Development

In general, majority of MOFs are powder, which limit their applications. In this study, the highly stable Zr-based MOF-808 and its thiol-modified MOF-808-SH were selected as precursors, and then they were wrapped by sodium alginate hydrogel via a dripping strategy. Based on these, MOF/hydrogel composite beads（Alg-MOF-808, AlgMOF-808-SH） with 3D network structure were prepared successfully. Due to its excellent adsorption properties and easy recovery, they were employed as effective environmental functional materials for the adsorption of Hg（Ⅱ）. The adsorption capacities of Hg（Ⅱ） were 170.52 mg·g-1and 221.73 mg·g-1respectively and they can achieve adsorption equilibrium in a short time. The adsorption stability of Alg-MOF-808-SH was studied at different p H as well. In addition, the removal efficiency of composite beads for Hg（Ⅱ） was almost unaffected by other coexisting ions, displaying good adsorption selectivity. Furthermore, the removal efficiency only decreased by 10.4% after 5 cycles, indicating that the as-prepared materials have high recycling efficiency. The results demonstrated that the MOF/hydrogel composite beads have a certain application potential for mercury removal in actual wastewater.

关键词： Environmental functional material Hg (II) adsorption MOF-808 thiol groups beads

Microarray of DNA probes on carboxylate functional beads surface

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Science China Chemistry 2000年第4期43卷 435-442页

作者：黄承志李原芳黄新华范美坤 Institute of Environmental Chemistry Southwest Normal University Chongqing 400715 China

The microarray of DNA probes with 5′-NH2 and 5′-Tex/3′-NH2 modified terminus on 10 μm carboxylate functional beads surface in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) is characterized in the present paper. It was found that the microarray capacity of DNA probes on the beads surface depends on the pH of the aqueous solution, the concentration of DNA probe and the total surface area of the beads. On optimal conditions, the minimum distance of 20 mer single-stranded DNA probe microarrayed on beads surface is about 14 nm, while that of 20 mer double-stranded DNA probes is about 27 nm. If the probe length increases from 20 mer to 35 mer, its microarray density decreases correspondingly. Mechanism study shows that the binding mode of DNA probes on the beads surface is nearly parallel to the beads surface.

关键词： DNA probe polystyrene beads microarray hybridization.

Single-cell protein profiling in microchambers with barcoded beads

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Microsystems & Nanoengineering 2019年第1期5卷 112-122页

作者： Lucas Armbrecht Rafael Sebastian Muller Jonas Nikoloff Petra Stephanie Dittrich Department of Biosystems Science and Engineering ETH ZurichMattenstrasse 264058 BaselSwitzerland

Single-cell profiling provides insights into cellular behaviour that macroscale cell cultures and bulk measurements cannot *** the context of personalized cancer treatment,the profiling of individual tumour cells may lead to higher success rates for therapies by rapidly selecting the most efficacious ***,genomic analysis at the single-cell level is available through highly sensitive sequencing ***,the identification and quantification of intracellular or secreted proteins or metabolites remains ***,we introduce a microfluidic method that facilitates capture,automated data acquisition and the multiplexed quantification of proteins from individual *** microfluidic platform comprises 1026 chambers with a volume of 152 pL each,in which single cells and barcoded beads are *** demonstrated multiplexed single-cell protein quantification with three different mammalian cell lines,including two model breast cancer cell *** established on-chip immunoassays for glyceraldehyde-3-phosphate dehydrogenase(GAPDH),galectin-3(Gal-3)and galectin-3 binding protein(Gal-3bp)with detection limits as low as 7.0×10^(4),2.3×10^(5)and 1.8×10^(3)molecules per cell,*** three investigated cell types had high cytosolic levels of GAPDH and could be clearly differentiated by their expression levels of Gal-3 and Gal-3bp,which are important factors that contribute to cancer *** it employed commercially available barcoded beads for this study,our platform could be easily used for the single-cell protein profiling of several hundred different ***,this versatile method is applicable to the analysis of bacteria,yeast and mammalian cells and nanometre-sized lipid vesicles.

关键词： beads sized selecting

MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically encoded hydrogel beads using a simple single-layer microfluidic device

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Microsystems & Nanoengineering 2020年第1期6卷 145-157页

作者： Yinnian Feng Adam K.White Jamin B.Hein Eric A.Appel Polly M.Fordyce Department of Genetics Stanford UniversityStanfordCA 94305USA Department of Bioengineering Stanford UniversityStanfordCA 94305USA Department of Biology Stanford UniversityStanfordCA 94305USA The Novo Nordisk Foundation Center for Protein Research Faculty of Health and Medical SciencesUniversity of CopenhagenBlegdamsvej 3b2200 CopenhagenDenmark Department of Materials Science&Engineering Stanford UniversityStanfordCA 94305USA Stanford ChEM-H Stanford UniversityStanfordCA 94305USA Chan Zuckerberg Biohub San FranciscoCA 94110USA

The widespread adoption of bead-based multiplexed bioassays requires the ability to easily synthesize encoded microspheres and conjugate analytes of interest to their ***,we present a simple method(MRBLEs 2.0)for the efficient high-throughput generation of microspheres with ratiometric barcode lanthanide encoding(MRBLEs)that bear functional groups for downstream surface *** production in MRBLEs 2.0 relies on the manual mixing of lanthanide/polymer mixtures(each of which comprises a unique spectral code)followed by droplet generation using single-layer,parallel flow-focusing devices and the off-chip batch polymerization of droplets into *** streamline downstream analyte coupling,MRBLEs 2.0 crosslinks copolymers bearing functional groups on the bead surface during bead *** the MRBLEs 2.0 pipeline,we generate monodisperse MRBLEs containing 48 distinct well-resolved spectral codes with high throughput(>150,000/min and can be boosted to 450,000/min).We further demonstrate the efficient conjugation of oligonucleotides and entire proteins to carboxyl MRBLEs and of biotin to amino ***,we show that MRBLEs can also be magnetized via the simultaneous incorporation of magnetic nanoparticles with only a minor decrease in the potential code *** the advantages of dramatically simplified device fabrication,elimination of the need for custom-made equipment,and the ability to produce spectrally and magnetically encoded beads with direct surface functionalization with high throughput,MRBLEs 2.0 can be directly applied by many labs towards a wide variety of downstream assays,from basic biology to diagnostics and other translational research.

关键词： beads spectral functional

Sorption of Pb(II) from aqueous solution by konjac glucomannan beads

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Science China(Technological Sciences) 2009年第1期52卷 223-226页

作者： LONG XiaoYan LUO XueGang WANG Yang LI Zheng School of Life Science and Engineering Southwest University of Science and TechnologyMianyang 621000China China Academy of Engineering Physics Mianyang 621000China

Konjac glucomannan beads have been investigated as metal biosorbent for Pb(II) from aqueous solutions. The effect of contact time, solution pH, initial metal concentration, and desorption were studied in batch experiments at 20°C±2°C. Maximum mental sorption was found to occur at initial pH 4.0–5.5. Kinetic studies revealed that the initial uptake was rapid and equilibrium was established in 3 h and that the data followed the prseudo-second order reaction. The equilibrium sorption data at initial pH 4.0 were described by the Langmuir and Freundlich isotherm models; however, Langmuir isotherm model has been found to provide the best correlation. The highest value of Langmuir maximum uptake (q max) was found to be 105.71 mg·g?1. Similar Freundlich empirical constant (K F) was obtained to be 1.98 for lead. Adsorption-complexation may be involved in the sorption process of lead. Desorption experiments showed evidence that after two contacts neither HCl nor EDTA solutions were able to desorb lead from the konjac glucomannan beads, but the desorbtion efficacy of HCl solution was higher than EDTA solution. The results obtained show that konjac glucomannan beads may be used for the treatment of wastewater contaminated with lead.

关键词： lead konjac glucomannan beads wastewater biosorption

Two kinds of ketoprofen enteric gel beads(CA and CS-SA) using biopolymer alginate

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Asian Journal of Pharmaceutical Sciences 2018年第2期13卷 120-130页

作者： Bingchao Cheng Dongyang Li Qiye Huo Qianqian Zhao Qi Lan Mengsuo Cui Weisan Pan Xinggang Yang Department of Pharmaceutics School of Pharmaceutical Sciences Shenyang Pharmaceutical University State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process

To obtain expected rapid-release and sustained-release of ketoprofen gel beads, this paper adopted biopolymer alginate to prepare alginate beads and chitosan-alginate gel beads. Formulation factors were investigated and optimized by the single factor test. The release of ketoprofen from calcium alginate gel beads in pH 1.0 hydrochloric acid solution was less than 10% during 2 h, then in pH6.8 was about 95% during 45 min, which met the requirements of rapid-release preparations. However, the drug release of chitosan-alginate gel beads in pH1.0 was less than 5% during 2 h, then in pH6.8 was about 50% during 6 h and reached more than 95% during 12 h, which had a good sustained-release behavior. In addition, the release kinetics of keteprofen from the calcium alginate gel beads fitted well with the Korsmeyer–Peppas model and followed a case-II transport mechanism. However, the release of keteprofen from the chitosan-alginate gel beads exhibited a non-Fickian mechanism and based on the mixed mechanisms of diffusion and polymer relaxation from chitosanalginate beads. In a word, alginate gel beads of ketoprofen were instant analgesic, while chitosan-alginate gel beads could control the release of ketoprofen during gastrointestinal tract and prolong the drug's action time.

关键词： Gel beads Enteric rapid-release Enteric sustained-release Ketoprofen

Extravascular use of drug-eluting beads: A promising approach in compartment-based tumor therapy

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World Journal of Gastroenterology 2013年第43期19卷 7586-7593页

作者： Simon Binder Andrew L Lewis J-Matthias Lhr Michael Keese Faculty of Medicine Mannheim University of Heidelberg68167 MannheimGermany Biocompatibles UK Ltd. Department for Research and DevelopmentFarnham GU98QL SurreyUnited Kingdom Karolinska Institute Department of Clinical ScienceIntervention and Technology17177 StockholmSweden Clinic for Vascular and Endovascular Surgery University Hospital Frankfurt60590 Frankfurt am MainGermany

Intraperitoneal carcinomatosis(PC)may occur with several tumor *** prognosis of patients suffering from PC is usually *** treatment depends on the cancer entity and includes systemic chemotherapy,radiation therapy,hormonal therapy and surgical *** few patients may also benefit from hyperthermic intraperitoneal chemotherapy with a complete tumor *** therapies are often accompanied by severe systemic *** approach to reduce side effects is to target chemotherapeutic agents to the tumor with carrier *** experimental results have been achieved using drug-eluting beads(DEBs).A series of in vitro and in vitro experiments has been conducted to determine the suitability of their extravascular *** encapsulation devices were able to harbor CYP2B1producing cells and to shield them from the hosts immune system when injected *** this way ifosfamide-which is transformed into its active metabolites by CYP2B1-could be successfully targeted into pancreatic tumor *** DEBs can be used to target chemotherapeutics into the abdominal cavity for treatment of *** CYP2B1 producing cells are proven to be save for usage in man and if local toxic effects of chemotherapeutics can be controlled,DEBs will become promising tools in compartmentbased anticancer treatment.

关键词： Compartment based therapy Intraperitoneal Drug-eluting beads Carcinomatosis Hyperthermic intraperitoneal chemotherapy Glioblastoma Pancreatic cancer CYP2B1 Ifosfamide

Efficacy of intra-arterial contrast-enhanced ultrasonography during transarterial chemoembolization with drug-eluting beads for hepatocellular carcinoma

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World Journal of Hepatology 2018年第1期10卷 95-104页

作者： Kazue Shiozawa Manabu Watanabe Takashi Ikehara Shuhei Yamamoto Takashi Matsui Yoshinori Saigusa Yoshinori Igarashi Iruru Maetani Division of Gastroenterology and Hepatology Department of Internal Medicine Toho University Medical Center Ohashi Hospital Division of Gastroenterology and Hepatology Department of Internal Medicine Toho University Medical Center Omori Hospital

AIM To assess the usefulness of intra-arterial contrastenhanced ultrasonography(IAUS) during transarterial chemoembolization(TACE) with drug-eluting beads(DEB) for hepatocellular carcinoma(HCC).METHODS Thirty two patients with 39 HCC underwent DEB-TACE guided with IAUS, and examined by contrast-enhanced ultrasonography(CEUS) or dynamic CT after DEB-TACE were enrolled in this study. CEUS findings before DEBTACE and IAUS findings were compared. Treatments judged to be complete and incomplete for lesions were appropriate and insufficient, respectively. Findings on CEUS and/or dynamic CT performed 1, 3 and 6 mo after DEB-TACE were evaluated using m RECIST(CR/PR/SD/PD).RESULTS The treatments were complete and incomplete in 26 and 13 lesions, respectively. On imaging evaluation using CEUS and/or dynamic CT one month after treatment, 25 and 1 lesions were judged to be CR and PR, respectively, and at 6 mo after treatment, the results were CR, PR, SD and PD for 24, 1, 0 and 1 of these lesions, respectively, in the 26 completely treated lesions. Of the 13 lesions in which treatment was incomplete, the results on imaging at one month after treatment were CR, PR, SD and PD for 0, 6, 4 and 3 lesions, respectively. The overall CR rate at 6 mo after treatment was 61.5%(24/39).CONCLUSION A combination of DEB-TACE with IAUS can improve the therapeutic effects in patients with HCC.

关键词： Hepatocellular carcinoma Contrast-enhanced ultrasonography Drug-eluting beads Transarterial chemoembolization Intra-arterial contrast-enhanced ultrasonography

同步辐射X射线显微成像研究环糊精自组装beads的三维结构

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药学学报 2018年第2期53卷 291-303页

作者：段效晖伍丽李雪徐建张国庆李海燕覃伟殷宪振张继稳安徽中医药大学药学院安徽合肥230012 中国科学院上海药物研究所上海201210

环糊精和油自组装形成的beads是一种新型的药物递送系统,在油类药物固体化、改善脂溶性药物生物利用度方面具有较强的应用潜力,但beads的形成机制仍不完善。本研究利用同步辐射X射线显微成像技术,测定beads的三维结构,基于对干燥前后be... 详细信息

环糊精和油自组装形成的beads是一种新型的药物递送系统,在油类药物固体化、改善脂溶性药物生物利用度方面具有较强的应用潜力,但beads的形成机制仍不完善。本研究利用同步辐射X射线显微成像技术,测定beads的三维结构,基于对干燥前后beads结构的定量分析,发现beads形成过程中Pickering乳滴的有序性。在添加脂溶性成分后,油相的表面张力改变,对beads的结构会产生影响。因此,在载脂溶性维生素K1的过程中beads的三维结构会发生改变,但依然可见beads形成过程中Pickering乳滴的有序性。本研究从beads内部三维结构出发,揭示了环糊精半包合形成Pickering乳滴,再有序聚集、组装形成beads的微观过程,完善了beads的形成机制,为beads的进一步研究提供了结构学基础。

关键词： beads 环糊精同步辐射X射线显微成像技术三维结构 Pickering乳维生素K1