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MODELING AND PARAMETER IDENTIFICATION FOR MULTISTAGE SIMULATION OF MICROBIAL BIOCONVERSION IN batch culture

International Journal of Biomathematics 2012年第4期5卷 177-188页

作者： LEI WANG ZHILONG XIU ZHAOHUA GONG ENMIN FENG School of Life Science and Biotechnology Dalian University of Technology Dalian 116023 Liaoning P. R. China School of Mathematics and Information Science Shandong Institute of Business and Technology Yantai 264005 Shandong P. R. China School of Mathematical Science Dalian University of Technology Dalian 116023 Liaoning P. R. China

The bioconversion of glycerol to 1,3-propanediol is a complex bioprocess. The item of product inhibition in the equation of Specific rate of cell growth is overlapped in [L. Wang, J. X. Ye, E. M. Feng and Z. L. Xiu, An improved model for multistage simula- tion of glycerol fermentation in batch culture and its parameter identification, Nonlinear Anal. Hybrid Syst. 3（4）（2009） 455462]. Therefore, in this work, the specific rate of cell growth is modified by the mechanism of multistage simulation of microbial bioconversion and the previous time-dependent model is converted to the autonomous standard form. The properties of the solutions for the nonlinear dynamic system are discussed and the identifiability of the parameters is proved. Finally the feasible optimization algorithm is constructed to find the optimal parameters for the system. Numerical result shows that the improved model with identified parameters can describe the batch culture better, compared with the previous results.

关键词： batch culture specific rate of cell growth nonlinear dynamical system parameters identification.

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Improved production of docosahexaenoic acid in batch fermentation by newly-isolated strains of Schizochytrium *** Thraustochytriidae *** bioprocess optimization

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Synthetic and Systems Biotechnology 2018年第2期3卷 121-129页

作者： Qiuzhen Wang Huike Ye Biswarup Sen Yunxuan Xie Yaodong He Sunghoon Park Guangyi Wang Center for Marine Environmental Ecology School of Environmental Science and EngineeringTianjin UniversityTianjin300072China Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjin300072China School of Energy and Chemical Engineering UNISTUlsanSouth Korea

Thraustochytrids,rich in docosahexaenoic acid(DHA,C22:6u3),represent a potential source of dietary fatty ***,the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid *** different thraustochytrid strains,Schizochytrium ***#Mn4 and Thraustochytriidae ***#Mn16 were studied for their growth and DHA production characteristics under various culture *** they exhibited similar fatty acid profiles,PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process *** DHA content of 48.5%and 49.2%(relative to total fatty acids),respectively,were achieved on glycerol under their optimal flask culture *** DHA yield(Yp/x)of 21.0%and 18.9%and productivity of 27.6 mg/L-h and 31.9 mg/L-h were obtained,respectively,in 5-L bioreactor fermentation operated with optimal conditions and dual oxygen control strategy.A 3.4-and 2.8-fold improvement of DHA production(g/L),respectively,was achieved in this ***,our study provides the potential of two thraustochytrid strains and their culture conditions for efficient production of DHA-rich oil.

关键词： Thraustochytrids Glycerol Polyunsaturated fatty acids Docosahexaenoic acids batch culture

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