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Effects of basic fibroblast growth factor on superoxide dismutase activity and malondialdehyde content in the rat brain following intracerebral hemorrhage

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Neural Regeneration Research 2008年第9期3卷 971-974页

作者： Hongqiao Wei Juen Huang Junjie Huang Bing Li Qianming Li Department of Physiology Guangxi Medical University Nanning 530021 Guangxi Zhuang Autonomous Region China Department of Histology and Embryology Guangxi Medical University Nanning 530021 Guangxi Zhuang Autonomous Region China Department of Physiology Youjiang Medical College for Nationafities Baise 533000 Guangxi Zhuang Autonomous Region China Department of Physiology Xinxiang Medical College Xinxiang 453000 He＇nan Province China

BACKGROUND： Studies have confirmed that basic fibroblast growth factor （bFGF） promotes neuronal survival and neurite outgrowth. OBJECTIVE： To compare and verify the effects of bFGF on superoxide dismutase activity and malondialdehyde content in rat brain tissues surrounding a hemorrhagic lesion, as well as the hippocampus at the hemorrhagic side. DESIGN, TIME AND SETTING： The randomized, controlled, neurobiological study was performed at the Science Experimental Center and Research Laboratory, Guangxi Medical University, China, from September to December 2006. MATERIALS： Ninety-two adult, healthy, Wistar rats of equal gender were used to establish intracerebral hemorrhage by infusing type VII collagenase into the left internal capsule. Type Ⅶ collagenase （Sigma, USA）, superoxide dismutase and malondialdehyde kits （Jiancheng, China）, and bFGF （Institute of Bioengineering, Ji＇nan University, China） were used for this study. METHODS： Ninety successfully lesioned rats were equally and randomly divided into three groups. Rats in the bFGF group were intramuscularly injected daily with bFGF （8 ug/kg）. Rats in the saline control group received an equal volume of saline. The rats in the model group did not receive other interventions. Superoxide dismutase activity was measured using the xanthine oxidase method. Malondialdehyde contents were detected using the thiobarbituric acid method. MAIN OUTCOME MEASURES： At 1, 3, and 7 days following intracerebral hemorrhage, superoxide dismutase and malondialdehyde were determined in the brain tissue surrounding the hematoma and in the hippocampus in the affected hemisphere. RESULTS： In brain tissue surrounding the hematoma, superoxide dismutase activity was significantly increased in the bFGF group at 3 and 7 days after intracerebral hemorrhage compared with the saline control group, whereas malondialdehyde content was significantly decreased （P 〈 0.05）. In the hippocampus, superoxide dismutase activity was significantly increased in

关键词： basic fibroblast growth factor intracerebral hemorrhage superoxide dismutase malondialdehyde

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Effect of early administration of exogenous basic fibroblast growth factor on acute edematous pancreatitis in rats

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World Journal of Gastroenterology 2006年第19期12卷 3060-3064页

作者： Qiang Yan Xing Yao Li-Cheng Dai Guo-Lei Zhang Jin-Liang Ping Jian-Fang He Chun-Fan Han Department of Hepatobiliary and Pancreatic Surgery Huzhou Central Hospital Huzhou 313000 Zhejiang Province China

AIM： To observe the therapeutic effect of early administration of exogenous basic fibroblast growth factor （bFGF） on acute edematous pancreatitis （AEP） in rats. METHODS： Thirty male Sprague-Dawley rats were randomly divided into three （n = 10）： normal control group （group Ⅰ）, AEP group （group Ⅱ） and AEP with bFGF treatment group （group Ⅲ）. AEP was induced by subcutaneous injection of cerulein （5.5 μg/kg and 7.5 μg/kg） at 1 h interval into rats of groups Ⅱ and Ⅲ. Three hours after induction of AEP, 100 μg/kg bFGF was administrated intraperitoneally for 1h to group Ⅲ rats. For test of DNA synthesis in acinar cells, 5-bromo-2＇-deoxyuridine （BrdU） labeling solution was intraperitoneally injected into the rats of groups Ⅱ and Ⅲ 24 h after bFGF treatment. The changes in serum amylase, lipase, pancreatic tissue wet/dry ratio were detected. RESULTS： In bFGF treatment group, there was a significant decrease in the volume of serum amylase, lipase and the pancreatic wet/dry weight ratio（1383.0 ± 94.6 U/L, 194.0 ± 43.6 U/L, 4.32 ± 0.32） compared to AEP group （3464 ± 223,7 U/L, 456 ±68,7 U/L, 6.89 ± 0,47）（P 〈 0.01）, and no significant difference was found between bFGF treatment and control group （1289 ± 94.0 U/L, 171 ± 23.4 U/L, 4.12 ± 0.26, P 〉 0.05）. The inflammatory changes such as interstitial edema, polymorphonuclear neutrophUs （PMNs） and vacuolization were significantly ameliorated compared to AEP group （P 〈 0.01）. A small number of BrdU-labeled nuclei were observed in acinar cells of AEP rats （1.8 ± 0.3 nuclei/microscopic field, n = 10） while diffuse BrdU-labeled nuclei were found in bFGF-treated rats （18.9 ± 1.4 nuclei/microscopic field, n = 10）（P 〈 0.01）. Immunohistochemical study showed increased DNA synthesis in pancreatic acinar cells. CONCLUSION： Early administration of exogenous bFGF has significant therapeutic effect on cerulein-induced acute edematous pancreatitis in rats. Its mechanism is related to the amelioration of inflammation and facilitation of pa

关键词： basic fibroblast growth factor Acute edematous pancreatitis Cerulein

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Effects of Electromagnetic Field and basic fibroblast growth factor on Osteoblast's growth

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Chinese Journal of Biomedical Engineering(English Edition) 2004年第3期13卷 133-138页

作者： GUOYong ZHANGXi-zheng WANGHao LIBin LIRui-xin WUJin-hui ZHAOYun-shan WUJi-min InstituteofMilitaryMedicalEquipment AcademyofMilitaryMedicalSciences.TianJin300161China

Osteoblasts of rat cultured in vitro were stimulated with pulsed 50 Hz electromagnetic field and basic fibroblast growth factor(bFGF).The MTT method,flow cytometry and histochemistry staining were used to detect cell proliferation?cell cycle and alkaline phosphatase .The results indicated: after stimulated by 1 mT electromagnetic field,the cells are more abundant,have more S phase percentages,2 mT electromagnetic field have no evident effect on cells’growth;compared with electromagnetic field,the cells stimulated by bFGF are more abundant and have larger S phase *** field and bFGF have no effect on cells,alkaline *** ,we concluded that electromagnetic field can enhance osteoblasts growth like some growth factor such as basic fibroblast growth factor,and the osteoblasts’,characteristics was not changed.

关键词： Electromagnetic field basic fibroblast growth factor Osteoblast

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Expression of basic fibroblast growth factor in Rat Liver Fibrosis and Hepatic Stellate Cells

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2005年第2期25卷 166-169,222页

作者：彭小东王波王天才赵秋 Department of Gastroenterology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Summary:The expression of basic fibroblast growth factor (bFGF) in rat liver fibrosis and hepatic stellate cells (HSCs) and the relationship between the expression of bFGF and rat liver fibrogenesis were studied. Sixty male SD rats (230-260 g) were divided into 4 groups randomly (the 0 week group, 1 week group, 4 week group and 8 week group). Liver fibrosis was induced by subcutaneous injection of carbon tetrachloride. The sections of rats' liver in each group were tested by Van-Gieson (V-G) staining and immunohistochemistry. The expression of bFGF mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). HSCs were isolated by the combined methods of collagenase IV perfusion and density gradient centrifugation. The expression of bFGF protein in cultured HSCs was detected by Western blot. Images of immunohistochemistry detection, agarose gel electrophoresis of RT-PCR and SDS-polyacrylamide gel electrophoresis of Western blot were analyzed semiquantitatively by image-analyzing system. The results were analyzed by statistics. The results showed that the fibers were gradually increased in the sections of rat liver with the prolongation of the model induction. At the end of the 8th weeks, liver fibrosis was formed. The expression of bFGF detected by immunohistochemistry showed a similar tendency of gradual increase. At the end of the 8th weeks, the bFGF expression could be observed in many regions in sections and the strongest expression was in interstitial cells including HSCs and some hepatocytes in regions around the portal area and central veins. Also there was moderate expression widely in extracellular matrix (ECM). In RT-PCR detection and Western blot detection of HSCs cultured in vitro, the similar tendency of gradual increase was evident either. It is suggested that bFGF is related with liver fibrosis of rats closely and may be a fibrogenesis factor of liver. bFGF possibly regulates liver fibrogenesis through regulating metabolism of extrace

关键词： basic fibroblast growth factor rat hepatic stellate cells liver fibrosis

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Effects of Cordyceps militaris extract on angiogenesis and tumor growth

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Acta Pharmacologica Sinica 2004年第5期25卷 115-123页

作者： Hwa-seung YOO,Jang-woo SHIN,Jung-hyo CHO,Chang-gue SON,Yeon-weol LEE,Sang-yong PARK,Chong-kwan CHO Daejeon University Daejeon 301-724 Department of East-West Cancer Center College of Oriental Medicine Daejeon University Daejeon 301-724 Department of East-West Cancer Center College of Oriental Medicine Daejeon University Daejeon 301-724 Department of East-West Cancer Center R&D Lab of Bulrolife Co Ltd Daejeon Korea College of Oriental Medicine Daejeon University Daejeon 301-724 Institute ofTraditional Medicine and Bioscience Daejeon University Daejeon 301-724 Department of East-West Cancer Center College of Oriental Medicine

AIM: To evaluate the effects of Cordyceps militaris extract (CME) on angiogenesis and tumor growth. METHODS:Human umbilical vein endothelial cells (HUVEC), HT1080, and B16-F10 cells were used. DNA fragment, angio-genic related gene expressions (MMPs, bFGF, VEGF, etc), capillary tube formation, wound healing in vitro, tumorgrowth in vivo were measured. RESULTS: CME inhibited growth of HUVECs and HT1080 (Pgrowth of B16-F10 melanoma cells in mice compared withcontrol group (P<0.05). CONCLUSION: CME has antiangiogenetic properties.

关键词： Cordyceps militaris angiogenesis human umbilical vein endothelial cells HT1080 cells B16-F10cells matrix metalloproteinases basic fibroblast growth factor

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Acupuncture promotes functional recovery after cerebral hemorrhage by upregulating neurotrophic factor expression

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Neural Regeneration Research 2020年第8期15卷 1510-1517页

作者： Dan Li Qiu-Xin Chen Wei Zou Xiao-Wei Sun Xue-Ping Yu Xiao-Hong Dai Wei Teng Third Department of Acupuncture First Affiliated HospitalHeilongjiang University of Chinese MedicineHarbinHeilongjiang ProvinceChina Department of Acupuncture Third Affiliated HospitalBeijing University of Chinese MedicineBeijingChina

Acupuncture is widely used in the treatment of cerebral hemorrhage,and it improves outcomes in experimental animal models and ***,the mechanisms underlying the effectiveness of acupuncture treatment for cerebral hemorrhage are still *** this study,a model of intracerebral hemorrhage was produced by injecting 50μL autologous blood into the caudate nucleus in Wistar *** at Baihui(DU20)and Qubin(GB7)acupoints was performed at a depth of 1.0 inch,12 hours after blood injection,once every 24 *** needle was rotated at 200 r/min for 5 minutes,For each 30-minute session,needling at 200 r/min was performed for three sessions,each lasting 5 *** the positive control group,at 6 hours,and 1,2,3 and 7 days after induction of hemorrhage,the rats were intraperitoneally injected with 1 mL aniracetam(0.75 mg/mL),three times a *** Bederson behavioral test was used to assess palsy in the contralateral *** blot assay was used to examine the expression levels of Nestin and basic fibroblast growth factor in the basal *** was performed to count the number of Nestin-and glial cell line-derived neurotrophic factor-positive cells in the basal *** effectively reduced hemorrhage and brain edema,elevated the expression levels of Nestin and basic fibroblast growth factor in the basal ganglia,and increased the number of Nestin-and glial cell line-derived neurotrophic factor-positive cells in the basal ***,these findings suggest that acupuncture promotes functional recovery after cerebral hemorrhage by increasing the expression of neurotrophic *** study was approved by the Committee for Experimental Animals of Heilongjiang Medical Laboratory Animal Center(approval No.2017061001)on June 10,2017.

关键词： acupuncture basic fibroblast growth factor brain cell protection cerebral hemorrhage electron microscope glial cell line-derived neurotrophic factor immunohistochemistry Nestin western blot assay

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Panax ginseng extract attenuates neuronal injury and cognitive deficits in rats with vascular dementia induced by chronic cerebral hypoperfusion

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Neural Regeneration Research 2018年第4期13卷 664-672页

作者： Jun-De Zhu Jun-Jie Wang Xian-Hu Zhang Yan Yu Zhao-Sheng Kang Department of Anatomy School of Basic MedicineGuizhou Medical University

Panax ginseng is a slow-growing perennial *** ginseng extract has numerous biological activities,including antitumor,anti-inflammatory and antistress *** ginseng extract also has a cognition-enhancing effect in rats with alcohol-induced memory *** this study,we partially occluded the bilateral carotid arteries in the rat to induce chronic cerebral hypoperfusion,a wellknown model of vascular *** rats were then intragastrically administered 50 or 100 mg/kg Panax ginseng *** water maze and balance beam tests were used to evaluate memory deficits and motor function,*** quantity was used to evaluate cholinergic *** staining was used to assess the number of glial fibrillary acidic protein-positive *** blot assay was used to evaluate protein levels of vascular endothelial growth factor,basic fibroblast growth factor,Bcl-2 and *** with Panax ginseng extract for 8 weeks significantly improved behavioral function and increased neuronal density and VEGF and b FGF protein expression in the hippocampal CA3 ***,Panax ginseng extract reduced the number of glial fibrillary acidic protein-immunoreactive cells,and it decreased apoptosis by upregulating Bcl-2 and downregulating Bax protein *** effect of Panax ginseng extract was dose-dependent and similar to that of nimodipine,a commonly used drug for the treatment of vascular *** findings suggest that Panax ginseng extract is neuroprotective against vascular dementia induced by chronic cerebral hypoperfusion,and therefore might have therapeutic potential for preventing and treating the disease.

关键词： nerve regeneration Panax ginseng extract chronic cerebral hypoperfusion vascular dementia learning and memory vascular endothelial growth factor basic fibroblast growth factor apoptosis neural regeneration

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Changes of Expressions of VEGF, bFGF, and Angiogenesis, and Effect of Benazepril, bFGF on Angiogenesis in Acute Myocardial Infarction Model of the Rabbits

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Biomedical and Environmental Sciences 2004年第4期17卷 442-451页

作者： DAN-YANGLI KUIZHAO JUN-FUZHOU PENGCHEN WEILI SecondAffiliatedHospital CollegeofMedicineZhejiangUniversity88JiefangRoadHangzhou310009ZhefiangChina TheFirstAffiliatedHospital CollegeofMedicineZhejiangUniversityHangzhou310009ZhefiangChina

Objective To explore the changes of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and angiogenesis, and the effects of bFGF, angiotensin converting enzyme inhibiter(ACEI) benazepril on the angiogenesis in acute myocardial infarction (AMI) model of rabbits, and to provide a probable evidence for the treatment of AMI. Methods AMI model was established by ligating anterior descending branch of coronary artery of Japan-Sino hybridization white rabbits. The postoperative rabbits were randomly divided into 6 groups and each group was treated with different drugs. Groups 1 and 2 were treated with normal saline (NS) for 28 and 14 days (d), group 3 and 4 with bFGF for 28 and 14 d, groups 5 with benazepril for 14 d, and group 6 with benazepril and bFGF for 14 d respectively. The rabbits were killed on the 14th or 28th d and their hearts were excised, sectioned and stained with HE, Masson trichrome to observe VEGF, bFGF and CD34 under a microscope, which were quantified with a computer-assisted morphometry. Results Compared with group 1, the granulation tissue of infarction zone (IZ) in group 2 freshened up, and the capillary density (CD) in IZ was increased (P=0.002). The CD in the IZ as well as VEGF and bFGF in groups 3 and 4 were increased respectively (P=0.011-0.037). In group 5 the changes of VEGF and bFGF were not found in the IZ and the border zone (BZ) while CD was significantly increased (35.4% and 25.6%, P=0.036 and 0.037). Compared with group 2, the CD in the IZ and BZ of group 6 was significantly increased (63.4% and 44.3% P=0.007 and 0.007), meanwhile VEGF and bFGF were increased. Compared with group 5, only VEGF was increased. Conclusion Intravenous bFGF may increase VEGF and bFGF significantly, thus promoting the angiogenesis in the IZ and BZ in cardiac infarction as VEGF and bFGF are the potent angiogenic growth factors. Benazepril may promote angiogenesis in the IZ and BZ in cardiac infarction, but its mechanism is irr

关键词： Myocardial infarction Angiogenesis basic fibroblast growth factor Vascular endothelial growth factor Benazepril

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Specific bFGF targeting of KIM-1 in ischemic kidneys protects against renal ischemia-reperfusion injury in rats

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Regenerative Biomaterials 2022年第1期9卷 335-346页

作者： Siqi Song Xianglin Hou Weiwei Zhang Xinyu Liu Wei Wang Xiaoya Wang Wenxuan Cao Yujun Xia Wei Chen Chunying Shi Department of Human Anatomy Histology and EmbryologySchool of Basic MedicineQingdao UniversityQingdao 266071China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100190China Department of Nephrology Army Medical Center of PLAArmy Medical UniversityChongqing 400038China Department of Urology Xinqiao HospitalArmy Medical UniversityChongqing 400038China

Renal ischemia-reperfusion(I/R)injury is one of the major causes of acute kidney ***,there is still no effective treatment for this *** fibroblast growth factor(bFGF)has been reported to be beneficial for recovery from ischemic *** is vital to increase the local concentration and reduce the diffusion of bFGF in vivo for renal I/R injury therapy.A targeted growth factor delivery system that responds to specific biological signals in the regenerative environment to guide release has been highlighted in tissue *** the present study,a specific peptide was fused with bFGF and called bFGF-kidney injury targeting(KIT-bFGF),and this compound specifically targeted kidney injury molecule-1 both in hypoxic renal HK-2 cells in vitro and ischemic kidneys in vivo after intravenous *** administered to rat models of renal I/R injury,KIT-bFGF attenuated renal tubule damage and fibrosis,and promoted functional recovery compared to the effects of native bFGF and the *** also investigated the mechanism by which KIT-bFGF activated the ERK1/2 and Akt signaling pathways to significantly reduce apoptosis and protect against ischemic injury in the *** results demonstrated that targeted delivery of KIT-bFGF could be an effective strategy for the treatment of renal I/R injury.

关键词： basic fibroblast growth factor acute kidney injury targeted therapy growth factor delivery system

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Hypoxia response element-directed expression of bFGF in dental pulp stem cells improve the hypoxic environment by targeting pericytes in SCI rats

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Bioactive Materials 2021年第8期6卷 2452-2466页

作者： Sipin Zhu Yibo Ying Yan He Xingxing Zhong Jiahui Ye Zhiyang Huang Min Chen Qiuji Wu Yifan Zhang Ziyue Xiang Yurong Tu Weiyang Ying Jian Xiao Xiaokun Li Qingsong Ye Zhouguang Wang Department of Orthopaedics The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang325000China Molecular Pharmacology Research Center School of Pharmaceutical ScienceWenzhou Medical UniversityWenzhouZhejiangChina Department of Pain Medicine The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou325000ZhejiangChina School and Hospital of Stomatology Wenzhou Medical UniversityWenzhou325035China Laboratory of Regenerative Medicine Tianyou HospitalWuhan University of Science and TechnologyWuhan430064China The Department of Cardiology The First Affiliated HospitalSchool of MedicineZhejiang UniversityChina Massachusetts General Hospital Harvard UniversityBoston02114USA Research Units of Clinical Translation of Cell growth factors and Diseases Research Chinese Academy of Medical ScienceChina Center of Regenerative Medicine Renmin Hospital of Wuhan UniversityWuhan630060China

Cell-based transplantation strategies possess great potential for spinal cord injury(SCI)*** fibroblast growth factor(bFGF)has been reported to have multiple neuro-promoting effects on developing and adult nervous system of mammals and considered a promising therapy for nerve injury following *** dental pulp stem cells(DPSCs)are abundant stem cells with low immune rejection,which can be considered for cell replacement *** purpose of this study was to investigate the roles of DPSCs which express bFGF under the regulation of five hypoxia-responsive elements(5HRE)using an adeno-associated virus(AAV-5HRE-bFGF-DPSCs)in SCI repairing *** this study,DPSCs were revealed to differentiate into CD13^(+)pericytes and up-regulate N-cadherin expression to promote the re-attachment of CD13^(+)pericytes to vascular endothelial *** re-attachment of CD13^(+)pericytes to vascular endothelial cells subsequently increased the flow rate of blood in microvessels via the contraction of *** a result,increased numbers of red blood cells carried more oxygen to the damaged area and the local hypoxia microenvironment in SCI was ***,this study represents a step forward towards the potential use of AAV-5HRE-bFGF-DPSCs in SCI treatment in clinic.

关键词： Spinal cord injury Adeno-associated virus basic fibroblast growth factor Dental pulp stem cell Vascular regulation Hypoxic microenvironment

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