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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第7期8卷 476-485页

作者： HUANG You-zong WEI Kang YANG Juan DAI Fei ZHANG Guo-ping Agronomy Department Zhejiang University Hangzhou 310029 China College of Agriculture Guangxi University Nanning 530004 China

Interaction of salinity （NaCI） and cadmium （Cd） on growth, mineral nutrients, Na and Cd accumulation in four barley genotypes differing in salt tolerance was studied in a hydroponic experiment. Cd, NaCI and their combined stresses reduced Ca and Mg concentrations in roots and shoots, K concentration in shoots, increased K and Cu concentrations in roots relative to control, but had non-significant effect on micronutrients Cu, Fe and Mn concentrations in shoot. The three stresses reduced accumulation of most tested nutrients in both roots and shoots, except NaCI and NaCl＋Cd stresses for root K and shoot Cu accumulation in salt tolerant genotypes. The salt tolerant genotypes did not have higher nutrient concentration and accumulation than the sensitive ones when exposed to Cd and NaCI stresses. In conclusion, the affecting mechanism of Cd stress on nutrients was to some extent different from salinity stress, and the NaCl＋Cd stress was not equal to additional Cd and NaCI stresses, probably due to the different valence and competitive site of Na^＋ and Cd^2＋. NaCI addition in the Cd-containing medium caused remarkable reductions in both Cd concentration and accumulation, with the extent of reduction being also dependent on genotypes. The salt-tolerant genotypes had lower Na concentration than sensitive ones.

关键词： barley Cadmium Growth Mineral nutrient Salinity

Aluminium tolerance in barley (Hordeum vulgare L.): physiological mechanisms, genetics and screening methods

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第10期7卷 769-787页

作者： WANG Jun-ping RAMAN Harsh ZHANG Guo-ping MENDHAM Neville ZHOU Mei-xue Tasmanian Institute of Agricultural Research University of Tasmania P.O. Box 46 Kings Meadows TAS 7249 Australia Department of Primary Industries Wagga Wagga Agricultural Institute PMB Wagga Wagga NSW 2650 Australia Department of Agronomy Zhejiang University Hangzhou 310029 China

Aluminium (Al) toxicity is one of the major limiting factors for barley production on acid soils. It inhibits root cell division and elongation, thus reducing water and nutrient uptake, consequently resulting in poor plant growth and yield. Plants tolerate Al either through external resistance mechanisms, by which Al is excluded from plant tissues or internal tolerance mechanisms, conferring the ability of plants to tolerate Al ion in the plant symplasm where Al that has permeated the plas- malemma is sequestered or converted into an innocuous form. barley is considered to be most sensitive to Al toxicity among cereal species. Al tolerance in barley has been assessed by several methods, such as nutrient solution culture, soil bioassay and field screening. Genetic and molecular mapping research has shown that Al tolerance in barley is controlled by a single locus which is located on chromosome 4H. Molecular markers linked with Al tolerance loci have been identified and validated in a range of diverse populations. This paper reviews the (1) screening methods for evaluating Al tolerance, (2) genetics and (3) mechanisms underlying Al tolerance in barley.

关键词： barley Al toxicity A1 tolerance

The Effects of Genotype and Mannitol Pretreatment on High Frequency Androgenesis in barley

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Chinese Science Bulletin 1993年第2期38卷 151-155页

作者：李文泽景健康姚根怀胡含 Laiyang Agricultural College Shandong 265200 PRC Institute of Genetics. Academia Sinica Beijing 100101 PRC

Since the success in induction of barley pollen plants through anther culture was achieved by Clapham in 1973, a great amount of efforts have been further made on the study. In the past 20 years, the green plant production has been increased by 100-fold by improving culture methods and media etc. However, there still exist a lot of theoretical and applied problems, for example, the green plantlet yield per anther for most materials except

关键词： anther culture of barley genotype mannitol pretreatment.

GREEN PLANT REGENERATION FROM PROTO PLASTS OF barley (Hordeum vulgate L.)

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Chinese Science Bulletin 1991年第11期36卷 932-935页

作者：颜秋生张雪琴施建表李浚明 China National Rice Research Institute Hangzhou 310006 PRC Beijing Agricultural University Being 100094 PRC

barley is the world’s fourth leading cereal in acreage planted, after wheat, rice and corn. Nevertheless, whilst plant regenerations from protoplast cultures of rice, corn and wheat were realized one after another during the past few years, only albinos were regenerated sporadically from barley protoplasts. The present note reports that a group of complete green plants has been obtained from protoplasts isolated from cell suspension cultures in barley.

关键词： barley (Hordeum vulgare L.) protoplast culture plant regeneration.

Betaine accumulation and the change of betaine-aldehyde dehydrogenase activity in barley seedlings under KCl and NaCl stress

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Journal of Environmental Sciences 1999年第4期11卷 415-418,442页

作者： YI Yan-jun XU Cheng-shui ZHAO Bo-sheng LIU Jia-yao WANG Xue-chen Department of Biology Qufu Normal UniversityQufu 273165China Department of Biology Zibo CollegeZibo 255013China College of Biology Chinese Agricultural Universi tyBeijing 100094China

The changes in growth status, ion contents, betaine levels and betaine\|aldehyde dehydrogenase (BADH) activity in seedlings of nonhalophyte barley treated with different concentrations of NaCl and KCl were studied. Results showed that the inhibition of growth in barley seedlings increased as enhancing NaCl and KCl for 96h and NaCl inhibitory degree was higher than that of KCl. The K\++ content of barley seedlings in NaCl was lower than the control, while the Na\++ content was higher, the levels of Na\++ fell in the seedlings treated with KCl, but K\++ levels rose, and K\++ content was higher than that of Na\++. The betaine levels of barley shoots rose with the increase in both external salt concentration and treatment time. Higher BADH activity was observed in low\|salt concentration but lower slightly in high\|salt concentration.

关键词： betaine betaine-aldehyde dehydrogenase(BADH) activity NaCl KCl barley

Identification of SNPs in barley(Hordeum vulgare L.)by deep sequencing of six reduced representation libraries

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The Crop Journal 2014年第6期2卷 419-425页

作者： Ganggang Guo Dawa Dondup Lisha Zhang Sha Hu Xingmiao Yuan Jing Zhang Key Laboratory of Crop Germplasm Resources and Utilization (MOA) The National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop Science Chinese Academy of Agricultural Sciences Tibet Academy of Agricultural and Animal Husbandry Sciences

High-density genetic markers are required for genotyping and linkage mapping in identifying genes from crops with complex genomes, such as barley. As the most common variation, single nucleotide polymorphisms(SNPs) are suitable for accurate genotyping by using the next-generation sequencing(NGS) technology. Reduced representation libraries(RRLs) of five barley accessions and one mutant were sequenced using NGS technology for SNP discovery. Twenty million short reads were generated and the proportion of repetitive sequences was reduced by more than 56%. A total of 6061 SNPs were identified, and 451 were mapped to the draft sequence of the barley genome with pairing reads. Eleven SNPs were validated using length polymorphic allele-specific PCR markers.

关键词： barley SNP discovery Reduced representation libraries Allele-specific PCR

Moisture-dependent physical properties of barley grains

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International Journal of Agricultural and Biological Engineering 2009年第4期2卷 84-91页

作者： Mahmoud Tavakoli Hamed Tavakoli Ali Rajabipour Hojat Ahmadi Seyed Mohammad Taghi Gharib-Zahedi Department of Food Science and Technology Faculty of AgricultureUniversity of ZabolZabolIran Department of Agricultural Machinery Engineering Faculty of Agricultural Engineering&TechnologyUniversity of TehranP.O.Box 4111Karaj 31587-77871Iran Department of Food Science and Engineering Faculty of Agricultural Engineering&TechnologyUniversity of TehranKarajIran

Designing equipment for processing,sorting,sizing and other post-harvest operations of agricultural products requires information about their physical *** study was carried out to evaluate the effect of moisture content on some physical properties of barley *** levels of moisture content ranging from 7.34%to 21.58%(d.b.)were *** average length,width,thickness,arithmetic mean diameter,geometric mean diameter,thousand grain mass,sphericity,surface area and repose angle increased from 8.91 to 9.64 mm,3.30 to 3.74 mm,2.58 to 2.98 mm,4.93 to 5.45 mm,4.23 to 4.75 mm,44.48 to 51.30 g,47.55%to 49.35%,56.66 to 71.09 mm2 and 31.16°to 36.90°,respectively,as moisture content increased from 7.34%to 21.58%(d.b.).The bulk density,true density and porosity were found to decrease with increasing moisture *** static friction coefficient of the grains increased linearly against various surfaces(plywood,glass and galvanized iron sheet)as the moisture content *** all moisture content,the maximum friction was offered by plywood,followed by galvanized iron sheet and glass surface.

关键词： barley moisture content angle of repose coefficient of friction

Important Quality Attributes of Malt barley(Hordeum vulgare L.):Effect of Genetics,Environments and Agronomic Factors

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Agricultural Sciences in China 2003年第7期2卷 815-824页

作者： VirenderSardana ZHANGGuo-ping RegionalResearchStation PunjabAgriculturalUniversityGurdaspurIndia DepartmentofAgronomy ZhejiangUniversityHangzhou310029P.R.China

barley has been in use as food and fodder for thousands of years. and also used currently as the most important raw material in malting and brewing. The quality of malt barley is not much satisfied with the requirement by malting and brewing industry worldwide, although the great effort has been devoted to its improvement through breeding and agronomy. The quality of malt barley is involved in many physical and chemical traits, including hydrolytic enzymes. Of them, kernel protein content, beta-amylase activity, beta-glucan content and kernel plumpness have dramatic influence on malting or brewing quality. The expression of these quality parameters varies greatly in genotypes and is easily changed by diverse environments and agronomic practices. This paper reviewed the important role of various parameters influencing the quality of malt barley and the effect of different factors on them.

关键词： barley Cultivar Environment Management Quality

Effect of Initial Soluble Salt Composition of Saline Soil on Salinity Tolerance of barley Plant

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Pedosphere 1991年第4期1卷 355-362页

作者： SHENQI－RONG LIUZHAO－PU 等 DepartmentofSoilScienceandAgrochemistry NanjingAgriculturalUniversityNanjing210014（China） Department

A pot experiment was carried out on a marine saline soil to study the effect of initial soluble Na/Ca ratio of saline soil on the salinity tolerance of barley *** results showed that (1) the Na/Ca ratio affected significantly the dry weight of the plant at an earlier stage of growth,the critical values of initial Na/Ca ratio at which the plant could grow normally on soils containing salts of 2.5,3.5 and 4.5g kg^-1 were 30,20 and 15,respectively;(2)smaller Na/Ca ratio resulted in a considerable decrease in Na accumulation but a great increase in K accumulation in the barley plant;and (3) the plasmallema of barley leaf were badly injured when the Na/Ca ratio was more than 30 and the increase of Na content of plant caused an exudation of K from the leaf *** critical indexes were suggested for the cultivation of barley plant on marine saline soils and could be used as reference in the biological reclamation of marine saline soils.

关键词： barley initial soluble Na/ Ca ratio marine saline soil salinity tolerance salt content

A Transient Expression System for the Functional Assessment of Early Response Genes on the Powdery Mildew Infected barley or Wheat Leaves

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Agricultural Sciences in China 2003年第10期2卷 1061-1068页

作者： LI Ai-li (Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture/Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081,P.R.China) Key Laboratory of Crop Germplasm and Biotechnology Ministry of Agriculture/Institute of Crop Germplasm Resources Chinese Academy of Agricultural Sciences Beijing 100081 P. R. China

The principle and the basic steps of the transient assay system for the functional assessment of early response genes on the powdery mildew infected barley or wheat leaves were summarized in brief. The development of this technology and its extensive application were reviewed. Future studies on this approach were recommended in this paper.

关键词： Transient expression system Powdery mildew barley Wheat