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DNA barcodes in Fig Cultivars (Ficus carica L.) Using ITS Regions of Ribosomal DNA, the psbA-trnH Spacer and the matK Coding Sequence

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American Journal of Plant Sciences 2015年第1期6卷 95-102页

作者： Carlos Castro Alejandro Hernandez Luis Alvarado Dora Flores Agribiotecnología de Costa Rica S.A. Alajuela Costa Rica Instituto Tecnológico de Costa Rica (ITCR) Cartago Costa Rica

Molecular markers provide a useful method for genotype characterization and allow a high precision determination of the genetic relationship between cultivars and varieties. A system based on DNA sequences—which is known as DNA barcoding—will choose one or several standard loci which can be sequenced and compared to differentiate between species. In this research, the ITS, matK, and trnH-psbA sequences were evaluated for the molecular identification of seven F. carica genotypes, generating complete sequences for the first two loci, but unable to produce bidirectional sequences by using the trnH-psbA sequence. The ITS sequence presented the highest variation rates, while the phylogeny constructed with the matK sequence obtained the highest percentage of solved monophyletic groups. Through Pearson’s correlation analysis, it was possible to determine the existence of a significant correlation between the ITS region and psbA-trnH, and the matK and psbA-trnH sequences, but not between ITS and matK. The phylogenies constructed with the ITS + matK barcodes and ITS + matK + psbA-trnH presented the highest percentage for resolution. However, considering the cost efficiency and the facilitated recovery by using PCR, the matK + ITS combination is recommended.

关键词： Ficus carica DNA barcodes ITS psbA-trnH matK

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Cloud Computing Solution and Services for RFID Based Supply Chain Management

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Advances in Internet of Things 2013年第4期3卷 79-85页

作者： S. M. Khalid Jamal A. Omer Asiya Abdus Salam Qureshi Department of Computer Science and Software Engineering University of Hail Hail KSA Department of Computer Science UBIT University of Karachi Karachi City Pakistan

Deploying and Implementing RFID Systems for tracking and controlling products in supply chain management is not affordable for small and midsized companies due to its complexity, cost, and difficulties pertaining data management and maintenance. Cloud computing can be used to mitigate the risks associated with the implementation and deployment of RFID based on system in supply chain management because the complex system for data filtering, management and maintenance can be implemented on the cloud that otherwise requires the purchase of new hardware, staff and professional services.

关键词： Cloud Computing RFID Tags Readers barcodes

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