检索结果-南通市图书馆

Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha,Sri Lanka

引用

Asian Pacific Journal of Tropical Biomedicine 2013年第5期3卷 381-387页

作者： Asha Dilrukshi Wijegunawardana Nilmini Silva Gunawardane Chanditha Hapuarachchi Aresha Manamperi Kithsiri Gunawardena Wimaladharma Abeyewickrama Molecular Medicine Unit Faculty of MedicineUniversity of Kelaniya Environmental Authority Department of Parasitology Faculty of MedicineUniversity of Kelaniya

Objective:To compare Wuchereria bancrofti(***)infection rates of Culex quinquefasciatus,using dissection and PCR-EUSA in two consecutive time periods(from 2007to 2008 and from 2008 to 2009).Methods:Mosquitoes were collected in 30 sentinel and 15 nonsentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of *** transmission in two consecutive time period of 2007 to 2008 and 2008 to *** mosquitoes were dissected to determine the *** larvae(L_1 L_2,L_3,).PCR was carried out using DNA extracted from mosquito pools(15 body parts/pool)utilizing the primers specific for Wb-Sspl *** products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific *** prevalence of infected/infective mosquiloes in PCR pools(3 pools/site)was estimated using the PoolSereen^(TM)algorithm and a novel probability—based ***:Of 45 batches of mosquitoes dissected,*** infected mosquitoes were found in19 and 13 batches,with an infection rate of 13.29%and 3.10%with mean larval density of 8.7 and1.0 larvae per mosquito for two study periods in the Gampaha *** of 405 pools of head,thorax and abdomen were processed by PCR-EUSA for each *** these,51 and 31 pools were positive for *** in the two study periods *** association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were0.176 and 0.890 respectively for the two ***:Data indicate that PCR-EUSA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.

关键词： Wuchereria bancrofti Culex quinquefasciatus Dissection PCR-ELISA Sri Lanka

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

bancroftiasis among the mumuye of Northeastern Nigeria: Parasitological and clinical studies in Northern Taraba State

引用

Health 2013年第1期5卷 138-142页

作者： Jacqueline A. Badaki Oladele B. Akogun Nobert B. Molta Godwin Imandeh Department of Biological Sciences Federal University of Technology Yola Nigeria Department of Biological Sciences University of Jos Jos Nigeria Department of Environmental Biology Adekunle Ajasin University Akungba-Akoko Nigeria

The study reports the prevalence of bancroftiasis in three Local Government Areas (LGAs) of Taraba State, Nigeria. Night blood samples collected from one thousand and thirty one individuals were stained with haematoxylin and examined microscopically for the presence of Wu-chereria bancrofti. The overall prevalence of infection in the study area was high (21.2%) but mean microfilaria densities were generally low. Statistically, prevalence rate differed significantly (χ2d.f.2 = 10.498;P χ2d.f.1 = 1.385;P > 0.05) between sexes. Infection also did not statistically differ between age groups (χ2d.f.5 = 13.219;P > 0.05) even though infection rate increased steadily with age and reaching a peak at the 40 - 49 year age group. Lymphoe-dema was the commonest (M = 12.4%, F = 19.2%) clinical manifestation of bancroftian morbidity encountered in the survey. Overall, the findings indicate that bancroftian filariasis is a public health problem in Taraba state and there is an urgent need to establish an elimination programme with a view of halting transmission in the area.

关键词： Wuchereria bancrofti Morbidity Microfilaraemia Mumuye Northeast Nigeria

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Historic accounts of Mansonella parasitaemias in the South Pacific and their relevance to lymphatic filariasis elimination efforts today

引用

Asian Pacific Journal of Tropical Medicine 2016年第3期9卷 201-207页

作者： J.Lee Crainey Tullio Romao Ribeiro da Silva Sergio Luiz Bessa Luz Ecologia de Doencas Transmissiveis na Amazonia Instituto Leonidas e Maria Deane-Fiocruz Amazonia Rua Terezina

There are two species of filarial parasites with sheathless microfilariae known to commonly cause parasitaemias in humans:Mansonella perstans and Mtinsonella *** most contemporary accounts of the distribution of these parasites,neither is usually considered to occur anywhere in the Eastern ***,Sir Patrick Manson,who first described both parasite species,recorded the existence of sheathless sharp-tailed Mansonella ozzardilike parasites occurring in the blood of natives from New Guinea in each and every version of his manual for tropical disease that he wrote before his death in ***'s reports were based on his own identifications and were made from at least two independent blood sample collections that were taken from the *** region Mansonella perstans parasitaemias were also later(in 1923) reported to occur in New Guinea and once before this(in 1905) in *** Mansonella-parasilaernias are generally regarded as benign,they are thought to be of public health importance because they can affect the epidemiological monitoring of other filarial *** this article,we reviewed the historic literature concerning Pacific-origin Mansonella-parasitaemias in an attempt to explain how,despite repeated reports of Pacificregion Mansonella-parasilaemias,by as early as the 1970 s,the WHO had arrived at the presentday view that Wuchereria bancrofti is the only cause of filarial parasitaemias in Papua New *** have also evaluated the evidence supporting the contemporary existence of Pacificarea parasitaemia-causing Mansonella parasites and assessed the relevance such parasites could have for present-day lymphatic filariasis elimination efforts in the region.

关键词： Mansonella ozzardi Mansonella perstans Wuchereria bancrofti Papua New Guinea Lymphatic filariasis Global Programme to Eliminate Lymphatic Filariasis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：