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A STUDY OF THE STRUCTURE AND THE EXPRESSION OF PROTOONCOGENES IN HUMAN PRIMARY GASTRIC CANCER

Chinese Journal of Cancer Research 1990年第1期2卷 4-11页

作者：陈诗书钱关祥陈季王敦瑞章有章孔良曼赵涵芳何尧祥汪肖钢薛建静钱晓岚于丽莉王璐 Department of Biochemistry Laboratory of Molecular Biology Shanghai Second Medical University Department of Biochemistry Laboratory of Molecular Biology Shanghai Second Medical Universityhe allelic distribution of EcoRI and BamHI fragments of ras family genes between the human primary gastric cancer tissues and the corresponding adjacent normal tissues did not show any differences. Three genotypes of BamHI restriction fragments length polymorphism of c-H-ras were revealed. No significant differences in the RFLPs were observed between normal individuals and gastric cancer patients. Four protooncogenes c-H-ras N-ras c-myc and c-fos were found to be transcriptionally active in the gastric cancer tissues in some cases examined. The comparison of the expression of these oncogenes between the malignant tissues and the corresponding normal tissues showed differential patterns. The expression of c-H-ras at cellular level was detected with in situ hybridization. The enhanced expression of c-H-ras in the gastric cancer cells was demonstrated but the degree of the expession among the cancer cells was shown to be heterogeneous. In addition the enhanced expression of c-H-ras was seen in the inflammatory cells.

The allelic distribution of EcoRI and bamhi fragments of ras family genes between the human primary gastric cancer tissues and the corresponding adjacent normal tissues did not show any differences. Three genotypes of bamhi restriction fragments length polymorphism of c-H-ras were revealed. No significant differences in the RFLPs were observed between normal individuals and gastric cancer patients. Four protooncogenes, c-H-ras, N-ras, c-myc and c-fos, were found to be transcriptionally active in the gastric cancer tissues in some cases examined. The comparison of the expression of these oncogenes between the malignant tissues and the corresponding normal tissues showed differential patterns. The expression of c-H-ras at cellular level was detected with in situ hybridization. The enhanced expression of c-H-ras in the gastric cancer cells was demonstrated, but the degree of the expession among the cancer cells was shown to be heterogeneous. In addition, the enhanced expression of c-H-ras was seen in the inflammatory cells.

关键词： A STUDY OF THE STRUCTURE AND THE EXPRESSION OF PROTOONCOGENES IN HUMAN PRIMARY GASTRIC CANCER EcoRI rRNA bamhi DNA

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油桐尺蠖核型多角体病毒的一种对bamhi不敏感的毒株

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微生物学报 1985年第4期 370-371页

作者：丁达明李先强周咏芝中国科学院武汉病毒所

已经分离到一种限制性内切酶不同于油桐尺蠖核型多角体病毒广西株(Buzura suppressariaNuclear Polyhedrosis Virus Guong Xi简称BsNPVGX)的新毒株——广东株(BsNPV GD)。BsNPV

关键词：酶谱内切酶 GD DNA 限制性 bamhi 毒株

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TWO EXPRESSED COPIES OF GLYCININ Gy4 GENE SUBFAMILY IN WILD SOYBEAN Glycine soja SHI

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Science China Chemistry 1987年第12期 1289-1297页

作者：薛中天徐美琳庄乃亮沈纬沈善炯 Jiaotong University Shanghai Institute of Plant Physiology Laboratory of Molecular Genetics Shanghai Laboratory of Molecular Genetics and Biotechnology Academia Sinica

To study the structure of genes encoding seed storage proteins of the wild variety of soybean （Glycine soja） in comparison with those of the cultivated （Glycine max）,cDNA libraries from cotyledon poly（A）RNA of both wild and cultivated soybeans were constructed.Six clones were isolated using the genomic clone λS312 which contains the coding sequence for the glycinin subunit precursor （AAB） as probe for molecular hybridization. Two clones pWS228 and pWS242 contain the full length of cDNA.Restriction mapping of the cloned cDNA and Southern hybridization of the cDNA with genomic DNA showed two expressed copies of glycinin Gy4 gene subfamily in wild soybean Glycine soja SHI. Alteration of DNA arrangement in group-Ⅱ glycinin gene subfamily during embryogenesis was also demonstrated.

关键词： DNA TWO EXPRESSED COPIES OF GLYCININ Gy4 GENE SUBFAMILY IN WILD SOYBEAN Glycine soja SHI PWS bamhi RNA TCC gene

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CLONING AND CHARACTERIZATION OF TWO CODING SEQUENCES FOR STORAGE PROTEIN IN SOYBEAN Glycine max

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中国科学B辑(英文版) 1986年第5期 503-515页

作者：薛中天徐美琳庄乃亮沈纬沈善炯 Laboratory of Molecular Genetics Shanghai Institute of Plant Physiology Academia Sinica Laboratory of Molecular Geneties and Bioteennology Jiao Tong Univertiy Shanghai Academia Sinica

Two coding sequences for storage protein in Glycine max have been cloned. They were characterized by molecular hybridization and in vitro translation. One sequence happens to hybridize to two sized mRNAs which are translated in vitro to 60 Kd and 42 Kd polypeptides corresponding to a and β subunits of 7S storage protein, and the other is hybridizable with 0.7×10~6 dalton mRNA giving rise to 57 Kd and 49 Kd polypeptides in vitro translation.

关键词： Glycine hybridize translated giving sized soybean cloned DNA bamhi Phage

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EXPRESSION OF HUMAN αD INTERFERON GENE IN PROKARYOTIC CELLS

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Chinese Science Bulletin 1984年第6期29卷 842-846页

作者：侯云德李应霞周建华杨新科张智清刘新垣 Institute of Virology Chinese Academy of Medical Sciences Beijing Shanghai Institute of Biochemistry Academia Sinica

Interferon system is shown to be an importent one of the stabilization of vertebrate cell functions. Results obtained in its clinical application in recent 10 years indicate that interferons have some effects on the t... 详细信息

关键词： vertebrate stabilization plasmid Interferon interferon promoter insert stranded bamhi digestion

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INFECTIOUS DISEASE

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China Medical Abstracts(Internal Medicine) 2000年第1期17卷 10-13页

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CONSTRUCTION OF A GENOMIC LIBRARY FROM A SOLID HUMAN ESOPHAGUS CANCER AND PRELIMINARY CHARACTERIZATION OF THE CLONED c-Ha-ras 1 PROTO-ONCOGENE

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Chinese Science Bulletin 1987年第11期32卷 768-772页

作者：徐洪基周红奕 China-Japan Friendship Hospital Beijing China-Japan Friendship Hospital Beijingesearches on oncogenes from human malignant tumors have terminated the long history in which mankind groped and hesitated in the dark to conquer cancer and have become the milestone of the modern molecular oncology. An established genomic library from a solid tumor or a tumor cell line could be screened by sib-selection to search for unknown oncogenes and even antioncogenes (tumor suppressors). Furthermore from

Researches on oncogenes from human malignant tumors have terminated the long history in which mankind groped and hesitated in the dark to conquer cancer and have become the milestone of the modern molecular oncology. An established genomic library from a solid tumor or a tumor cell line could be screened by sib-selection to search for unknown oncogenes and even antioncogenes (tumor suppressors). Furthermore,

关键词： genomic library bamhi terminated mankind milestone screened oncology Phage DNA

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原核细胞中人神经生长因子基因的克隆

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中国医科大学学报 2003年第2期32卷 97-99页

作者：尹元琴马萍隋承光任常山中国医科大学基因工程研究中心辽宁沈阳110001 中国医科大学附属第一医院肿瘤研究所分子肿瘤研究室

目的:克隆人神经生长因子cDNA,为神经生长因子cDNA的表达作准备。方法:采用大肠杆菌JM 109菌株作为宿主菌,以pUC 118作为克隆载体,使用限制性内切酶bamhi,PstI酶切DNA片段,采用磷酸钙转染法转化细菌,α互补和限制性酶切图谱鉴定转化细... 详细信息

目的:克隆人神经生长因子cDNA,为神经生长因子cDNA的表达作准备。方法:采用大肠杆菌JM 109菌株作为宿主菌,以pUC 118作为克隆载体,使用限制性内切酶bamhi,PstI酶切DNA片段,采用磷酸钙转染法转化细菌,α互补和限制性酶切图谱鉴定转化细菌。结果:βNGF cDNA经bamhi和PstI 双酶切后,经过重组,与载体连接在一起,转化大肠杆菌后,形成了克隆。结论:应用JM 109作为宿主,pUC 118为克隆载体,成功克隆了人神经生长因子cDNA。

关键词：神经生长因子克隆原核细胞 cDNA 限制性内切酶 bamhi 载体

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PHYSICAL MAPPING OF TRANSPOSON Tn233(CH)

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Chinese Science Bulletin 1984年第2期29卷 256-262页

作者：洪孟民王宗阳陈剑民张景六蔡瑞珠杨蕴刘郑霏琴 Shanghai Institute of Plant Physiology Academia Sinica

Transposon Tn 233(CH) encoding resistance to streptomycin, sulfonamide and mercuric salt was originally resident on Shigella flexneri plasmid DR233. In order to study its molecular characters, Tn233(CH) was transposed... 详细信息

关键词： plasmid bamhi encoding Shigella originally agarose resident EcoRI characters cleavage

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A RECOMBINANT PLASMID WITH INCREASED COPY NUMBER IN Bacillus subtilis

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Chinese Science Bulletin 1985年第3期30卷 400-404页

作者：范树田杨迪孙永华李明风汤懋竑 Instituts of Genetics Academia Sinica Beijing Student of Beijing Normal University. Instituts of Genetics Academia Sinica Beijingnee the eloning system of B.subtilis has sPeeial advantagesit becomes an important system for eloning and expression of foreign genest

Sinee the eloning system of B.subtilis has sPeeial advantages,it becomes an important system for eloning and expression of foreign

关键词： bamhi plasmid Plasmid DNA Agarose purified Ehrlich suspended inserted designated

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