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Study of primary leiomyosarcoma induced by MNNG in balb/c nude mice

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World Journal of Gastroenterology 2000年第1期6卷 128-130页

作者： Zhu JS Su Q Zhou JG Hu PL Xu JH Institute of Oncology Hengyang Medical CollegeHengyang 421001China

INTRODUcTIONIt has been well known that MNNG is one of thestrong and multipotential carcinogens that havebeen frequently reported inducing malignant peptictumors.We have successfully induced rat and doggastric adenoca... 详细信息

关键词： Subject headings leiomyosarcoma stomach neoplasms MNNG balb/c nude mice

Mouse-adapted SARS-coV-2 replicates efficiently in the upper and lower respiratory tract of balb/c and c57BL/6J mice

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Protein & cell 2020年第10期11卷 776-782页

作者： Jinliang Wang Lei Shuai chong Wang Renqiang Liu Xijun He Xianfeng Zhang Ziruo Sun Dan Shan Jinying Ge Xijun Wang Ronghong Hua Gongxun Zhong Zhiyuan Wen Zhigao Bu State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150069 China National High containment Laboratory for Animal Diseases control and Prevention Harbin 150069 China

Dear Editor,As of June,2020,more than ten million cases of cOVID-19 have been reported worldwide.The causative pathogen of the disease is a novel coronavirus named severe acute respiratory syndrome coronavirus 2(SARS-coV-2)(World Health Organization,2020).Animal infection models are important to characterize the infection,pathogenesis,and immunology of SARS・coV・2,as well as for the development of medications and vaccines against cOVID-19.Mice are particularly attractive animal models for their identical genetic background,reliable reproducibility,well characterized biology,and the huge availability of research reagents and knockout animals.Models in inbreed mice such as balb/c and c57BL/6J(c57),which are widely used in research,are highly desired.

关键词： c57BL/6J balb/c respiratory

clinical hematological and biochemical parameters in Swiss,balb/c,c57BL/6 and B6D2F1 Mus musculus

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Animal Models and Experimental Medicine 2020年第4期3卷 304-315页

作者： Giorgio Silva-Santana Juliet cunha Bax Débora cristina Silva Fernandes Daniela Tendler Leibel Bacellar cleber Hooper Alexandre Alves Souza Oliveira Dias cristina Barbosa Silva Aline Moreira de Souza Simone Ramos Ricardo Alexandre Santos Thainara Ramos Pinto Mariana Antunes Ramão Ana Luíza Mattos-Guaraldi Health Sciences center Institute of Microbiology Paulo de GóesFederal University of Rio de JaneiroRio de JaneiroRJBrazil Laboratory of Diphtheria and corynebacteria of clinical Relevance Faculty of Medical SciencesUniversity of the State of Rio de JaneiroRio de JaneiroRJBrazil The collaborating centre for Reference and Research on Diphtheria/National Health Foundation/Ministry of Health Rio de JaneiroRJBrazil Laboratory of clinical Research and Molecular Diagnostic Prof.Marcílio Dias do Nascimento Department of Clinical Practice and PathologyFluminense Federal UniversityNiteróiRJBrazil Laboratory Bacterial Vaccines Department of ImmunologyNational Institute for Quality Control in HealthOswaldo Cruz FoundationRio de JaneiroRJBrazil Institute of Science and Technology in Biomodels Oswaldo Cruz FoundationRio de JaneiroRJBrazil Institute of Biology Federal Fluminense UniversityNiteróiRJBrazil Biomedical center College of Medical SciencesUniversity of the State of Rio de JaneiroRio de JaneiroRJBrazil

Background:Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions.Various lineages of mice have been used to investigate diseases and new therapeutic strategies,and,consequently,hematological and biochemical tests in these laboratory animals are essential to validate scientific studies.Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice.Methods:We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss(heterogeneous),balb/c and c57BL/6(isogenic),and B6D2F1(hybrid),totaling 160 mice.Analysis were standardized using the systems pocH-100iV Diff™for 19 hematological parameters and VITROS®350 for 12 biochemical parameters.Results:Results are shown as means and standard deviation,grouped by lineage and genre.comparing the values obtained in this study with the values from previous studies,some variations were detected,which could be explained by differences in methodologies or individual variability.conclusion:Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary,and emphasises the importance of considering variations influenced by gender,lineage and genotype in the choice of the best experimental model.

关键词： B6D2F1 balb/c biochemical c57BL/6 hematological Swiss

PcR AMPLIFIcATION, MOLEcULAR cLONING,DNA SEQUENcE ANALYSIS AND IMMUNO/PROTEcTION IN balb/c MIcE OF THE 33 kDa ENDOFLAGELLAR PROTEIN OF L.INTERRORGANS SEROVAR LAI

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chinese Medical Sciences Journal 1997年第1期12卷 15-21页

作者：戴保民陈庄阎和平赵慧业李胜富 Research Unit of Leptospirosis Department of Pathophysiology West China University of Medical Sciences Chengdu 610044.

A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PcR and inserted into plasmid pUc8,after the fragment and pUc8 were digested respectively with BamHI and PstI. A recombinant plasmid (designated as pLF1 ) was obtained. SDS-PAGE analysis indicated that a 33 kDa was expressed in E. coli JM103 harboring pLF1 and the expression level of the protein was 11 % of the total bacterial soluble proteins. Western blot analysis showed that the protein band could be recognized by the antiserum against the endoflegella (Axial filament ) of Leptospira interrt,gans serover lai. Nucleotide sequence data showed an open reading frame encoding 282 aminoacids residues,corresponding to a protein of molecular weight 33. 6kDa. comparison of the deduced endoflagellar subunit protein (flaB) amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Trehoema pallid um flaB proteins. Immunization/protection experiment was performed on the model of balb/c mice and showed that there was higher survival rate in the group JM103-pLE1 than in the group JM103-pUc8.

关键词： PcR扩增分子克隆基因序列序列分析 balb/c BamH1 pLFI JM103-pLEI JM103-pUc8

肝螺杆菌感染对四氯化碳诱导balb/c小鼠肝损伤的影响

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肝螺杆菌感染对四氯化碳诱导BALB/c小鼠肝损伤的影响

作者：喻虹锦扬州大学

学位级别：硕士

背景:肝损伤是临床上一种常见的肝脏疾病。四氯化碳（ccl4）作为一种常见化学物质可诱导肝损伤的发生,急性肝损伤可引发肝衰竭,而持续性的轻度肝损伤会导致肝纤维化及肝硬化,严重威胁人类的健康。肝螺杆菌（Helicobacter hepaticus,H.h... 详细信息

背景:肝损伤是临床上一种常见的肝脏疾病。四氯化碳（ccl4）作为一种常见化学物质可诱导肝损伤的发生,急性肝损伤可引发肝衰竭,而持续性的轻度肝损伤会导致肝纤维化及肝硬化,严重威胁人类的健康。肝螺杆菌（Helicobacter hepaticus,H.h）是一种主要定植于宿主肠道内革兰氏阴性菌,属于弯曲菌目螺杆菌科螺杆菌属。研究表明,高脂饲养等环境应激可促进宿主体内的H.h从肠道向肝脏转移。但关于H.h感染在慢性肝纤维化和急性肝损伤中的作用尚不明确。本研究拟采用balb/c小鼠为动物模型,通过联合H.h感染和cc14处理,探讨H.h感染对ccl4致急慢性肝损伤病程的影响。目的与方法:（1）为探讨H.h感染对ccl4致balb/c小鼠慢性肝损伤的影响,本研究将雄性balb/c小鼠（5-6周龄,40只）随机分为4组（对照组、H.h组、ccl4组、H.h+ccl4组）,实验周期为12周:对照组进行正常饲喂;H.h组采用H.h溶液灌胃处理,每3天一次;cc14组使用40%ccl4溶液进行腹腔注射处理,每3天一次;H.h+ccl4组于H.h灌胃结束1周后,进行ccl4处理。分别于ccl4注射后的4周、12周进行采样。检测小鼠体内H.h肝脏定植量、血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）含量;分别采用苏木精-伊红染色法（HE）和天狼星红染色法观察小鼠肝脏纤维化病变;采用免疫组化法和荧光定量（RT-qPcR）检测小鼠肝脏中IL-6、IL-33、α-SMA、TNF-a和TBF-β的表达。（2）为探讨H.h感染对ccl4致balb/c小鼠急性肝损伤的影响,本研究将雄性balb/c小鼠（5-6周龄,40只）随机分为4组（对照组、H.h组、ccl4组、H.h+ccl4组）:对照组进行正常饲喂;H.h组采用H.h灌胃处理,每3天一次,H.h感染持续8周;ccl4组在第8周时,使用40%ccl4溶液进行一次腹腔注射处理;H.h+ccl4组于H.h感染8周后,用40%ccl4溶液进行一次腹腔注射。分别于ccl4注射后的2 d、5 d、9 d和12 d进行采样,检测指标与慢性实验相同。结果:（1）慢性实验结果显示,处理4周时,cc14组和H.h+ccl4组小鼠均出现轻度肝纤维化,其他组未见明显病变。处理12周时,与H.h组相比,H.h+ccl4组小鼠肝脏中H.h定植量显著升高,与cc14组相比,H.h+ccl4组小鼠血清中ALT和AST均显著升高,肝脏中胶原纤维沉积量增多,炎症和纤维化相关因子转录水平显著升高。（2）急性实验结果显示,在处理2 d、5 d和12 d时,ccl4组与H.h+ccl4组各类数据无明显差异。9d时,与ccl4相比,H.h+ccl4组小鼠血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）含量升高,苏木精-伊红染色法（HE）、天狼星红染色观察到急性肝损伤加剧;免疫组化和蛋白质印迹法（Western blot）检测IL-33抗体和α-SMA蛋白表达量升高,荧光定量（RT-qPcR）检测IL-6、TNF-α、TGF-β、iNOS、IL-4等细胞因子转录水平升高。结论:综上表明,ccl4处理能够促进H.h在小鼠肝脏中定植,同时H.h感染能够加剧ccl4诱导的balb/c小鼠肝纤维化病变,抑制ccl4致急性肝损伤的修复。本研究可以为进一步揭示肝损伤疾病的发病机制提供理论依据。

关键词：肝螺杆菌 ccl4 balb/c 肝损伤肝纤维化

Synergetic effect of Egyptian propolis in immunization of balb/c mice against bovine cysticercosis

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Asian Pacific Journal of Tropical Biomedicine 2015年第4期5卷 324-330页

作者： Omnia Mohamed Kandil Somia Ayesh Nassar Soad Mohamed Nasr Hatem Abdel Mawgoud Shalaby Seham Hendawy Faragalla Mohamed El Moghazy Department of Parasitology and Animal Diseases National Research Centre Biology Department College of Science and Humanity Studies Salman Bin Abdul Aziz University

Objective: To evaluate the synergetic effect of an ethanolic extract of Egyptian propolis in immunization of balb/c mice with Taenia saginata(T. saginata) crude antigen against bovine cysticercosis, with reference to its effects on liver and kidney functions.Methods: Sixty female mice balb/c strain weighing 20 to 25 g and 6-8 weeks old were randomly allocated into six groups of ten mice each. Mice in groups 1 and 2(G1 and G2) were immunized intraperitoneally with 100 μg of T. saginata crude antigen in 100 μL phosphate buffer saline emulsified in Freund's adjuvant. Besides, the mice in G2 were administered with propolis extract simultaneously with immunization. control mice were either administered with propolis extract(G3) or injected with the same volume of phosphate buffer saline emulsified in Freund's adjuvant(G4). The mice in G5 were non-immunized infected control while, those in G6 were non-immunized non-infected control. Two weeks after the last immunization, each mouse was challenged intraperitoneally with 5 000 oncospheres except those of G6. Ethanolic extract of propolis was prepared at a dose 50 mg/kg body weight.Results: After 24 weeks of challenge, the mice in G2 showed the highest level of protection(100%), with no cyst being detected rather than mice in G1(33.3% protection). Additionally,the ELISA results, in this study, showed higher antibody titer in G2 with reduction the alteration in liver and kidney functions compared to G1.conclusions: Egyptian propolis could increase the level of protection against experimental challenge infection with T. saginata eggs when administered simultaneously with immunization. Furthermore, it could enhance the production of antibodies to immunized antigen and decrease the alteration in liver and kidney functions.

关键词： Taenia saginata Vaccine balb/c mice ELISA Serum biochemistry

balb/c和c57BL/6N小鼠局部过敏性鼻炎和耐受的研究

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BALB/c和C57BL/6N小鼠局部过敏性鼻炎和耐受的研究

作者：张启迪华中科技大学

学位级别：硕士

第一部分balb/c和c57BL/6小鼠局部过敏性鼻炎模型免疫反应的初步研究目的balb/c和c57BL/6是过敏性疾病研究中常用的小鼠品系。本研究目的是利用局部过敏性鼻炎模型研究两个小鼠品系之间的免疫学差异。方法balb/c和c57BL/6小鼠接受不同剂... 详细信息

第一部分balb/c和c57BL/6小鼠局部过敏性鼻炎模型免疫反应的初步研究目的balb/c和c57BL/6是过敏性疾病研究中常用的小鼠品系。本研究目的是利用局部过敏性鼻炎模型研究两个小鼠品系之间的免疫学差异。方法balb/c和c57BL/6小鼠接受不同剂量(0mg/ml,0.25mg/ml,25mg/ml)的卵清蛋白(OVA)进行为期8周的局部滴鼻。测量过敏症状、OVA特异性血清抗体(Ig E、Ig G1、Ig G2a)、脾脏培养上清中的细胞因子(IL-4、IFN-γ、IL-10)、局部鼻粘膜中浸润的嗜酸性粒细胞和杯状细胞和差异表达的基因。结果相同剂量的OVA刺激下,c57BL/6比balb/c过敏性症状加重更显著,balb/c和c57BL/6血清Ig E、Ig G1、Ig G2a逐渐增加,c57BL/6血清抗体升高程度更低,Ig G2a全程未升高,balb/c脾细胞培养上清IL-4、IL-10随剂量增高而增加,IFN-γ在0.25mg/ml剂量升高更显著,c57BL/6小鼠IL-4、IL-10、IFN-γ未升高,两种小鼠嗜酸性粒细胞和杯状细胞的浸润与剂量呈正比,而c57BL/6升高幅度大于balb/c,RNA-seq提示c57BL/6的固有免疫反应和免疫系统功能显著上调,JUN kinase通路和MAPKK通路上调。造成两种小鼠过敏性鼻炎免疫反应差异基因为Kng2、Kng1、Gnb3、Lpar3、Lpar1、Pik3r1、Pf4、Apob、Rps9、Fbxo2。结论balb/c小鼠和c57BL/6小鼠之间的免疫反应有显著差异。balb/c小鼠出现了较弱的局部过敏性炎症反应和强烈的全身性免疫反应。相反,c57BL/6小鼠的局部过敏性炎症反应更强,而全身免疫反应相对温和。因此,可以根据研究的重点选择不同的小鼠品种。第二部分balb/c和c57BL/6小鼠局部过敏性鼻炎耐受模型免疫应答差异的初步研究目的我们前期观察发现,balb/c和c57BL/6小鼠在局部过敏及耐受模型中,症状及免疫表征均有较大的差异。本研究旨在系统比较二者在局部耐受模型中的免疫差异情况,并对其产生机制进行探讨。方法我们使用一定剂量的(25mg/ml)OVA连续15周分别给balb/c小鼠、c57BL/6小鼠局部滴鼻。记录过敏性症状,检测小鼠血清特异性OVA抗体(Ig E、Ig G1、Ig G2a),检测脾脏细胞培养上清的细胞因子(IL-4、IL-10、IFN-γ)浓度,观察鼻黏膜嗜酸性粒细胞及杯状细胞的浸润,免疫组化的方法观察鼻粘膜TGF-β、IL-10、eotaxin的表达情况,用RNA-seq技术分析两种小鼠局部鼻粘膜的差异基因。结果在一定剂量抗原长期刺激下,balb/c小鼠表现为过敏性症状先加重后减轻,c57BL/6呈现症状持续加重。balb/c血清Ig E、Ig G1、Ig G2a随着滴鼻时间的延长而增加,c57BL/6血清Ig E、Ig G1有相似的趋势,但升高程度不及balb/c,Ig G2a则未见上升。balb/c体外培养脾脏细胞上清IL-4先上升后下降,IL-10保持上升,c57BL/6体外培养脾脏细胞上清各细胞因子未见显著变化。balb/c鼻粘膜嗜酸性粒细胞和eotaxin增多到一定程度保持不变,杯状细胞先增多后减少,鼻粘膜TGF-β、IL-10表达上调。c57BL/6鼻粘膜嗜酸性粒细胞、杯状细胞持续增多,15周时c57BL/6鼻粘膜中嗜酸性粒细胞、杯状细胞均多于balb/c,其eotaxin表达亦高于balb/c,TGF-β及IL-10未发生显著变化。GO-KEGG测序结果显示,15周时balb/c鼻粘膜免疫反应和炎症反应等生物学过程上调,并且耐受相关通路TGF-β信号通路,基因表达负调控通路上调。c57BL/6鼻粘膜中上调/下调改变的基因数量显著少于balb/c,其主要为细胞合成和分泌相关通路上调。两种小鼠差异排名前10的HUB节点:Rps27a,Uba52,Ubc,Kng2,Gnal,c3,Gng7,Gnb3,Reep5,Reep6。结论局部一定剂量卵清蛋白长期刺激可诱导balb/c小鼠形成局部耐受,而c57BL/6则不能产生局部耐受。与c57BL/6小鼠相比,balb/c小鼠TH2反应减轻,Treg及相关耐受反应增强。c57BL/6小鼠则显示TH2反应增强,Treg及耐受机制未见显著变化。局部及全身调节机制可能是局部耐受产生的重要原因,而该机制的缺失可能导致过敏持续加重,耐受无法产生。

关键词：过敏性鼻炎动物模型卵清蛋白 balb/c c57BL/6 免疫耐受

Novel DNA vaccine based on hepatitis B virus core gene induces specific immune responses in balb/c mice

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World Journal of Gastroenterology 2005年第29期11卷 4583-4586页

作者： Yi-Ping Xing Zu-Hu Huang Shi-Xia Wang Jie cai Jun Li Te-Hui W chou Shan Lu Department of Infectious Diseases the First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road Nanjing 210029 Jiangsu Province China Department of Medicine University of Massachusetts Medical School 55 Lake Avenue North Worcester MA 01655 United States

AIM: To investigate the immunogenicity of a novel DNA vaccine,pSW3891/HBc, based on HBV core gene in balb/c mice.METHODS: A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid pSW3891. balb/c mice were immunized with either pSW3891/HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (cTLs) of mice was quantitatively measured by lactate dehydrogenase release assay.RESULTS: HBcAg was expressed effectively in 293T cell line transiently transfected with pSW3891/HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891/HBc. The end-point titers of anti-HBc reached the highest 1:97 200, 4 wk after the third immunization. The specific cTL killing with the highest specific lysis reached 73.25% at effector:target ratio of 20:1 in mice that received pSW3891/HBc DNA vaccine.cONcLUSION: pSW3891/HBc vaccination elicits specific anti-HBc response and induces HBc-specific cTL response in immunized balb/c mice.

关键词： DNA疫苗乙型肝炎病毒免疫反应 balb/c 小鼠动物实验

Etoposide sensitizes cT26 colorectal adenocarcinoma to radiation therapy in balb/c mice

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World Journal of Gastroenterology 2005年第31期11卷 4895-4898页

作者： chia-Yuan Liu Hui-Fen Liao Tsang-En Wang Shee-chan Lin Shou-chuan Shih Wen-Hsuing chang Yuh-cheng Yang ching-chung Lin Yu-Jen chen Division of Gastroenterology Department of Internal Medicine Mackay Memorial Hospital Taipei Taiwan China Department of Medical Research Mackay Memorial HospitalTaipei Taiwan China Department of Molecular Biology and Biochemistry National Chiayi University Chiayi 300 Taiwan China Nursingand Management college Taipei Taiwan China Department of Radiation Oncology Mackay Memorial Hospital Taipei Taiwan China

AIM: To investigate the combined effect of etoposide and radiation on cT26 colorectal adenocarcinoma implanted into balb/c mice.METHODS: We evaluated the radiosensitizing effect of etoposide on cT26 colorectal adenocarcinoma in a syngeneic animal model. balb/c mice were subcutaneously implanted with cT26 cells and divided into four groups:control (intra-peritoneal salinex2) group, etoposide (5 mg/kgintra-peritoneallyx2) group, radiation therapy (RT 5 Gyx2fractions) group, and combination therapy with etoposide (5 mg/kg intra-peritoneally 1 h before radiation) group.RESULTS: Tumor growth was significantly inhibited by RT and combination therapy. The effect of combination therapy was better than that of RT. No significant changes were noted in body weight, plasma alanine aminotransferase,or creatinine in any group. The leukocyte count significantly but transiently decreased in the RT and combination therapy groups, but not in the etoposide and control groups. There was no skin change or hair loss in the RT and combination therapy groups.cONcLUSION: Etoposide can sensitize cT26 colorectal adenocarcinoma in balb/c mice to RT without significant toxicity.

关键词：感光性 cT26 结肠癌放射治疗 balb/c 小鼠动物实验