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Assessment of Expression Cassettes and Culture Media for Different Escherichia coli strains to Produce Astaxanthin

Natural Products and Bioprospecting 2018年第5期8卷 397-403页

作者： Shun Li Jun-Chao Huang Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China University of Chinese Academy of Sciences Beijing 100049People’s Republic of China

Astaxanthin is a value-added ketocarotenoid with great potential in nutraceutical and pharmaceutical *** engineering of heterologous hosts for astaxanthin production has attracted great *** this study,we assessed some key factors,including codon usage of the expressed genes,types of promoters,bacterial strains,and culture media,for engineered Escherichia coli to produce *** effect of codon usage was shown to be related to the types of *** DH5a was superior to other strains for astaxanthin *** culture media greatly affected the contents and yields of astaxanthin in engineered *** the expression cassette containing GadE promoter and its driving genes,HpCHY and CrBKT,was inserted into the plasmid pACCAR16DcrtX and expressed in *** DH5a,the engineered strain was able to produce 4.30±0.28 mg/g dry cell weight(DCW)or 24.16±2.03 mg/L of astaxanthin,which was a sevenfold or 40-fold increase over the initial production of 0.62±0.03 mg/g DCW or 0.61±0.05 mg/L.

关键词： Astaxanthin Escherichia coli bacterial strains Codon usage Medium

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Antibacterial activity of four Gracilaria species of red seaweeds collected from Mandapam Coast,Gulf of Mannar Marine Biosphere Reserve,India

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Journal of Coastal Life Medicine 2016年第9期4卷 703-707页

作者： Sethu Rameshkumar Kolidoss Radhakrishnan Arasan Sreenivasan Samraj Aanand Department of Marine Science Bharathidasan UniversityTiruchirapalli-620024Tamil NaduIndia Department of Fisheries Environment Fisheries College and Research InstituteTuticorin-628008Tamil NaduIndia

Objective:To study the antibacterial activities of diethyl ether,toluene,ethanol and methanol extracts of red seaweeds such as Gracilaria crassa(***),Gracilaria folifera(***),Gracilaria debilis(***)and Gracilaria ***:The crude extracts were tested against different types of Gram-positive and-negative bacterial strains and all the seaweed extracts were tested a broad spectrum of antibacterial *** activity was made using paper disc diffusion *** organic solvents(diethyl ether,toluene,methanol and ethanol)were used separately in a Soxhlet apparatus for seven bacterial *** activity of the known antibiotics such as chloramphenicol,streptomycin,kanamycin and ampicillin was determined by testing them against different test ***:The high antibacterial activity was noted in the extracts of ***,*** and ***,*** and *** have good antibacterial *** like Bacillus subtilis and Escherichia coli were less susceptible to the methanol and diethyl ether extracts of *** comparative study on the antibacterial activity was also made by using 200μg concentration of solvent extracts(diethyl ether,ethanol,toluene and methanol)and different five antibiotics such as chloramphenicol,streptomycin,kanamycin,amoxicillin and *** bacterial strains tested were more sensitive to chloramphenicol,streptomycin,kanamycin,and ampicillin when compared to algal ***:The present study proved that the extracts of ***,*** and *** have high antibacterial *** *** and *** showed good antibacterial activity,many known antibiotics are active against a few organisms ***,the extracts of seaweeds were active against all test organisms used and the activities were comparable to that of antibiotics and the seaweeds offer a feasible alternative for the development of new *** r

关键词： Seaweeds Gracilaria species Antibacterial activity bacterial strains Gram-positive and-negative bacteria

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