检索结果-南通市图书馆

Multicolor biomass based carbon nanodots for bacterial imaging

Chinese Chemical Letters 2022年第2期33卷 798-802页

作者： Wenbo Zhao Yong Wang Kaikai Liu Rui Zhou Chongxin Shan Henan Key Laboratory of Diamond Optoelectronic Materials and Devices Key Laboratory of Materials PhysicsMinistry of EducationSchool of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China

Biomass-based carbon nanodots(CNDs) are becoming promising fluorescent materials due to their superior optical properties and excellent biocompatibility. However, most fluorescent CNDs are prepared under high temperatures with artificial chemicals as precursors. In this work, multicolor biomass-based CNDs have been prepared by employing natural biomass as precursors through an ultrasonic-assisted method at room temperature. The multicolor biomass-based CNDs can be prepared within 10 min, and cavitation produced by ultrasound in solution contributes to the polymerization of biomolecules into nanodots. The emission of the CNDs covers from blue to red region, with emission peaks centered at 410 nm, 520 nm and 670 nm, and the corresponding photoluminescence quantum yields of the CNDs are 11%, 12% and28%, respectively. Furthermore, bacterial imaging by using the biomass-based CNDs as fluorescent imaging agent has been demonstrated. This work provides a convenient ultrasonic-assisted way for fabrication multicolor and eco-friendly biomass CNDs, demonstrating their application in bacterial imaging.

关键词： Carbon nanodots Biomass Fluorescence Ultrasonic methods bacterial imaging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A novel nitroreductase-enhanced MRI contrast agent and its potential application in bacterial imaging

引用

Acta Pharmaceutica Sinica B 2018年第3期8卷 401-408页

作者： Yun Liu Leilei Zhang Marc Nazare Qingqiang Yao Hai-Yu Hu School of Medicine and Life Sciences University of Jinan-Shandong Academy of Medical Sciences Institute of Materia Medica Shandong Academy of Medical Sciences Key Laboratory for Biotech-Drugs Ministry of HealthKey Laboratory for Rare & Uncommon Diseases of Shandong Province State Key Laboratory of Bioactive Substances and Function of Natural Medicine Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences Leibniz-Forschngsinstitut fvr Molekulare Pharmakologie (FMP) Campus Berlin-Buch

Nitroreductases(NTRs) are known to be able to metabolize nitro-substituted compounds in the presence of reduced nicotinamide adenine dinucleotide(NADH) as an electron donor. NTRs are present in a wide range of bacterial genera and, to a lesser extent, in eukaryotes hypoxic tumour cells and tumorous tissues, which makes it an appropriate biomarker for an imaging target to detect the hypoxic status of cancer cells and potential bacterial infections. To evaluate the specific activation level of NTR, great efforts have been devoted to the development of fluorescent probes to detect NTR activities using fluorogenic methods to probe its behaviour in a cellular context; however, NTR-responsive MRI contrast agents are still by far underexplored. In this study, para-nitrobenzyl substituted T_1-weighted magnetic resonance imaging(MRI)contrast agent Gd-DOTA-PNB(probe 1) has been designed and explored for the possible detection of NTR.Our experimental results show that probe 1 could serve as an MRI-enhanced contrast agent for monitoring NTR activity. The in vitro response and mechanism of the NTR catalysed reduction of probe 1 have been investigated through LC–MS and MRI. Para-nitrobenzyl substituted probe 1 was catalytically reduced by NTR to the intermediate para-aminobenzyl substituted probe which then underwent a rearrangement elimination reaction to Gd-DOTA, generating the enhanced T1-weighted MR imaging. Further, LC–MS and MRI studies of living Escherichia coli have confirmed the NTR activity detection ability of probe 1 at a cellular level. This method may potentially be used for the diagnosis of bacterial infections.

关键词： Nitroreductase MRI contrast agent Smart imaging probes bacterial imaging bacterial infection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Novel Nitroreductase-enhanced MRI Contrast Agent and its Potential Application in bacterial imaging

A Novel Nitroreductase-enhanced MRI Contrast Agent and its P...

引用

第十届全国化学生物学学术会议

作者： Yun Liu Leilei Zhang Marc Nazare Qingqiang Yao Hai-Yu Hu Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences

Nitroreductases（NTRs） are present in a wide range of bacterial genera and, to a lesser extent, in eukaryotes hypoxic tumour cells and tumorous tissues, which makes it an appropriate biomarker for an imaging target to detect the hypoxic status of cancer cells and potential bacterial infections. NTR-responsive MRI contrast agents are still by far underexplored. In this study, para-nitrobenzyl substituted T1-weighted magnetic resonance imaging（MRI） contrast agent Gd-DOTA-PNB（probe 1） has been designed and explored for the possible detection of NTR. Our experimental results show that probe 1 could serve as a MRI-enhanced contrast agent for monitoring NTR activity. Para-nitrobenzyl substituted probe 1 was catalytically reduced by NTR to the intermediate para-aminobenzyl substituted probe which then underwent a rearrangement elimination reaction to Gd-DOTA, generating the enhanced T1-weighted MR imaging. Further, LC-MS and MRI studies of living E. coli have confirmed the NTR activity detection ability of probe 1 at a cellular level. This method may potentially be used for the diagnosis of bacterial infections.

关键词： Nitroreductase MRI contrast agent Smart imaging probes bacterial imaging

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Cationic antibacterial metal nanoclusters with traceable capability for fluorescent imaging the nano–bio interactions

引用

Nano Research 2023年第1期16卷 999-1008页

作者： Yixiao Li Shaohua Qu Yumeng Xue Lianbing Zhang Li Shang School of Materials Science and Engineering Northwestern Polytechnical UniversityXi’an 710072China School of Life Sciences Northwestern Polytechnical UniversityXi’an 710072China Northwestern Polytechnical University Chongqing Technology Innovation Center Chongqing 400000China

A thorough understanding of antimicrobial mechanism is of great importance for developing novel,efficient antibacterial agents.While cationic nanoparticles,such as metal nanoclusters(NCs),represent an attractive type of antibacterial nanoagents,their interactions with bacteria remains largely un-elucidated.Herein,we report the synthesis of cationic bovine serum albuminprotected AuAgNCs(cBSA-AuAgNCs),which exhibit both near-infrared(NIR)fluorescence properties and significant antimicrobial effects.With E.coli and S.aureus as the representative bacteria,we investigated the antimicrobial process of cBSAAuAgNCs in real-time based on their intrinsic fluorescence properties via fluorescence imaging.Our results showed that these cBSA-AuAgNCs exert their antimicrobial effects primarily by attaching on the outer membrane of bacteria without obvious internalization,which is significantly different from the antibacterial process of negatively-charged metal NCs.Further mechanistic investigation showed that these cationic NCs will cause serious disruption to the bacterial membrane due to strong electrostatic interactions,which then leads to over accumulation of reactive oxygen species(ROS)that finally causes the bactericidal action.This study demonstrates the great potential of cationic luminescent metal NCs as novel,traceable antimicrobial agents,which also provides new tools for further understanding microbial interactions of nanomedicines.

关键词： metal nanoclusters antimicrobial agents bacterial imaging near-infrared fluorescence

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficient blue light-responsed dithienylethenes with exceptional photochromic performance

引用

Chinese Chemical Letters 2023年第3期34卷 392-395页

作者： Ziyong Li Xinyu Gao Haining Zhang Xiaoxie Ma Yifang Liu Hui Guo Jun Yin College of Food and Drug College of Chemistry and Chemical EngineeringLuoyang Key Laboratory of Organic Functional MoleculesLuoyang Normal UniversityLuoyang 471934China College of Chemistry Key Laboratory of Pesticide and Chemical BiologyMinistry of EducationHubei International Scientific and Technological Cooperation Base of Pesticide and Green SynthesisInternational Joint Research Center for Intelligent Biosensing Technology and HealthCentral China Normal UniversityWuhan 430079China

Three novel dithienylethenes modified by bifluoroboron β-diketonate fragments have been successfully developed. Upon blue light irradiation, they reached photostationary state within 2-5 s, as well as 100% conversion ratio and photocyclization quantum yield of > 0.70. Such fascinating photochromism were endowed by collaborative role of electron-withdrawing effect of BF_(2)bdk group to reduce HOMO-LUMO electronic gap for the open isomer, together with intramolecular hydrogen bonds and CH-π interactions favoring antiparallel conformation fixation. Moreover, they displayed specific discrimination and photoswitchable bacterial imaging for S. aureus.

关键词： Dithienylethene Photochromism Fluorescent switching behavior Visible light Bifluoroboronβ-diketonate(BF_(2)bdk) bacterial imaging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：