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Evaluating bacterial contamination and surgical site infection risks in intracorporeal anastomosis: Role of bowel preparation

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World Journal of Gastrointestinal Surgery 2024年第6期16卷 1953-1955页

作者： Junho Lee Department of Surgery Hanyang UniversityCollege of MedicineSeoul 04763South Korea

We recently read the study by Kayano et al on intracorporeal anastomosis(IA)for colon cancer,which assessed bacterial contamination and medium-term onco-logical outcomes and affirmed that IA is analogous to extracorporeal anastomosis in reducing intraperitoneal bacterial risk and achieving similar oncological *** commentary addresses gaps,particularly concerning bowel preparation and surgical site infections(SSIs),and highlights the need for comprehensive details on the bowel preparation methods that are currently employed,including mecha-nical bowel preparation,oral antibiotics(OA),their combination,and specific OA *** emphasize the necessity for further analyses that investigate these me-thods and their correlation with SSI rates,to enhance clinical protocol guidance and optimize surgical *** meticulous analyses are essential for refi-ning strategies to effectively mitigate SSI risk in colorectal surgeries.

关键词： Intracorporeal anastomosis Surgical site infection Mechanical bowel preparation Oral antibiotics bacterial contamination Colon cancer

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Molecular beacon-assisted isothermal amplification combined with nucleic acid lateral flow technology for the detection of multiple bacterial contaminations in platelets

Molecular beacon-assisted isothermal amplification combined ...

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2012全国临床微生物与感染免疫学术研讨会

作者： Wang Xiaohui,Wang Jidong,Li Yuan,Zhan Linsheng Beijing Institute of Transfusion Medicine

bacterial infections still pose a major risk for the safety of the blood supply,especially for the platelet concentrates(PCs),as they are stored at room temperature for 5-7 days.A lot of works based on culture method have been done to test the sterility of the PCs,but most of them have to take at least 24h which is too long when compare to the short shift time of the *** species of bacteria could contaminate the PCs,it is really hard to identify a kind of antigen or protein that conserved in all species of bacteria serologically if it is not impossible. So,more and more researchers tend to use molecular diagnostic technologies to solve these problems. A trend evident in disease diagnosis field in the past two decades is that the molecular diagnostic technologies such as polymerase chain reaction(PCR) are edging out the serological method,because they are rapid,specific and most importantly *** the requirement of the expensive equipment and skillful workers restricted this method to only be applied in medical center or research ***,several isothermal amplification methods had been invented since *** typical ones include Strand Displacement Amplification(SDA), Nucleic Acid Sequence Based Amplification(NASBA) and Loop-Mediated Isothermal Amplification(LAMP) etc. Without of the need of precision instrument and skillful worker,these methods could detect as low as one copy of target genes at isothermal condition in about 2 *** our knowledge,the 16S rDNA sequence exists usually in most types of bacteria and often provides general information for organism *** 16S rDNA-based realtime PCR method is also considered as a useful tool for rapid screening of bacterial contaminations in blood ***,we employed the conserved 24-base fragment in it as a detection *** the mentioned isothermal amplification methods are sensitive and easy to use,they also have some disadvantages of which the primer design is the mo

关键词： Beacon A mplification bacterial contamination Detection Platelets

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Assessing decontamination practices at a medical microbiology research laboratory

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Journal of Biosafety and Biosecurity 2022年第2期4卷 124-129页

作者： Merna M.Alfy Sherine B.El Sayed Mona El-Shokry Medical Microbiology and Immunology Department Faculty of MedicineAin Shams UniversityCairoEgypt

To our knowledge,this is the first study to conduct an objective assessment of the routine decontamination practices at a medical microbiology research laboratory(MRL)a year after a biosafety training was provided to all laboratory *** March 28th and June 28th,2021,unobtrusive observations were carried out to identify-three high-touch surfaces at the MRL during different working *** was used to evaluate the effectiveness of the disinfectant used in the *** three high-touch surfaces were sampled before and after decontamination with 200 ppm of 5%sodium hypochlorite(household bleach)to quantify the microbial load and identify the types of organisms residing on the laboratory surfaces.A higher concentration(500 ppm)of 5%sodium hypochlorite was employed after refresher training was provided to housekeeping staff,and resampling of the three surfaces was carried out during a 4-week follow-up period using the same *** three high-touch surfaces identified were the two sides of the workbench(22%–24%)and the front surface of one incubator(14%).Anthracoid bacilli and Staphylococcus aureus were the most commonly found organisms on laboratory surfaces preintervention(100%and 89%,respectively)and post-intervention(56%and 44%,respectively).Other microorganisms detected included Salmonella spp.(27.7%),Proteus spp.(5.6%),Escherichia coli(5.6%),and Klebsiella spp.(33.3%).Employing a higher concentration(500 ppm)of sodium hypochlorite significantly(pcontamination practices at the MRL and the need to apply a higher concentration(500 ppm)of sodium hypochlorite to reduce the overall microbial *** also demonstrated the importance of quantitative assessment to monitor decontamination practices and ensure staff *** studies are needed to identify bacterial communities within the laboratory,which will

关键词： Biosafety Laboratory-acquired infection Sodium hypochlorite Environmental decontamination bacterial contamination Egypt

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Portable Diagnostic Platform for Detection of Microorganisms Coliforms and ***

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Advances in Microbiology 2020年第5期10卷 224-237页

作者： Natália Faria Romao Nathalia Aparecida Costa Pereira Maribel Elizabeth Funes-Huacca Luciana Gatto Brito Rede de Biodiversidade e Biotecnologia da Amazonia Legal(BIONORTE) Fundacao Universidade Federal de Rondonia(UNIR)Porto VelhoBrazil Department of Chemistry Fundacao Universidade Federal de Rondônia(UNIR)Porto VelhoBrazil Empresa Brasileira de Pesquisa Agropecuária(EMBRAPA) BelémBrazil

Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.

关键词： Portable Device bacterial contamination Environment

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