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Mass spectrometry profiling of single bacterial cells reveals metabolic regulation during antibiotics induced bacterial filamentation

引用

Chinese Chemical Letters 2023年第6期34卷 242-246页

作者： Dongxue Zhang Qin Qin Liang Qiao Department of Chemistry Institutes of Biomedical Sciencesand Shanghai Stomatological HospitalFudan UniversityShanghai 200000China Changhai Hospital The Naval Military Medical UniversityShanghai 200433China

bacterial antimicrobial resistance(AMR)is a severe threat to global health and *** the stimulation of antibiotics,bacterial cells can undergo filamentation and generate daughter cells with stronger *** current research on bacterial AMR mechanism is mainly conducted with a population of ***,bacterial cells exhibit heteroresistance,making the study at population level not ***,we developed single bacterial cell metabolic profiling by mass spectrometry(MS)to study bacterial AMR at single-cell *** utilizing a microprobe controlled by a microoperation platform,single filamentous extended spectrum beta-lactamase(ESBL)producing Escherichia coli(***)cells generated by ceftriaxone sodium stimulation can be extracted and spray-ionized for MS *** among *** cells under the same antibiotic stimulus condition was observed from mass spectra as well as cell *** metabolic profiles by MS of different individual cells can be clustered into subgroups well in accordance with bacterial cell *** pathways including arginine and proline metabolism,as well as cysteine and methionine metabolism were disclosed to play an important role in the bacterial SOS-associated filamentation against *** microprobe electrospray ionization-MS-based single bacterial cell analysis method is promising in the study of various bacterial AMR mechanism and can reveal the heterogeneity of bacterial AMR from-cell-to-cell.

关键词： ESI-MS Single bacterial cell analysis Metabolome bacterial antimicrobial resistance bacterial filamentation

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