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Journal of Environmental Sciences 2008年第10期20卷 1210-1217页

作者： DU, Cheng WU, Zhenbin XIAO, Enrong ZHOU, Qiaohong CHENG, Shuiping LIANG, Wei HE, Feng State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate University Chinese Academy of Sciences Beijing 100039 China

The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCBI for identification purposes. The 90.6% of the clones were a?l...

关键词： bacterial diversity restriction fragment length polymorphism (RFLP) submerged membrane bioreactor (SMBR) 16S rDNA clone library

bacterial diversity and community structure in the East China Sea by 454 sequencing of the 16S rRNA gene

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Chinese Journal of Oceanology and Limnology 2014年第3期32卷 527-541页

作者：董逸赵苑张文燕李艳周锋刘诚刚吴莹刘素美张武昌肖天 Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of Sciences University of Chinese Academy of Sciences Energy Research Institute Shandong Academy of Sciences State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyState Oceanic Administration Laboratory of Marine Ecosystem and Biogeochemistry Second Institute of OceanographyState Oceanic Administration State Key Laboratory of Estuarine and Coastal Research East China Normal University Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education Ocean University of China

The 454 sequencing method was used to detect bacterial diversity and community structure in the East China Sea. Overall, 149 067 optimized reads with an average length of 454 nucleotides were obtained from 17 seawater samples and fi ve sediment samples sourced in May 2011. A total of 22 phyla, 34 classes, 74 orders, 146 families, and 333 genera were identifi ed in this study. Some of them were detected for the fi rst time from the East China Sea. The estimated richness and diversity indices were both higher in the sediment samples compared with in the seawater samples. All the samples were divided by their diversity indices into four regions. Similarity analysis showed that the seawater samples could be classifi ed into six groups. The groups differed from each other and had unique community structure characteristics. It was found that different water masses in the sampling areas may have had some infl uence on the bacterial community structure. A canonical correspondence analysis revealed that seawater samples in different areas and at different depths were affected by different environmental parameters. This study will lay the foundation for future research on microbiology in the East China Sea.

关键词： bacterial diversity community structure 454 sequencing water masses canonical correspondence analysis East China Sea

bacterial diversity in the sediment of Crescent Moon Spring,Kumtag Desert,Northwest China

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Journal of Arid Land 2017年第2期9卷 278-286页

作者： ZHANG Wei ZHANG Gaosen WU Xiukun LIU Guangxiu DONG Zhibao QU Jianjun WANG Yun CHEN Tuo Key Laboratory of Desert and Desertification Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China Gansu Key Laboratory of Extreme Environmental Microbial Resources and Engineering Lanzhou 730000China Dunhuang Gobi and Desert Ecology and Environment Research Station Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesDunhuang 736200China

Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs （Operational Taxonomic Units） in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI GenBank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems （including desert lakes） worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacterwere the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs （including OTU3615, OTU6535, and OTU6646） between sediment of Crescent Moon Spring and various origins from NCBI GenBank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other

关键词： bacterial diversity pyrosequencing analysis desert lake desert and lake ecosystems Crescent Moon Spring

bacterial diversity in sediments of core MD05-2902 from the Xisha Trough,the South China Sea

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Acta Oceanologica Sinica 2014年第10期33卷 85-93页

作者： LI Tao WANG Peng State Key Laboratory of Marine Geology Tongji University Guangzhou Marine Geological Survey

A sediment core MD05-2902 was collected from the deep-sea basin of the Xisha Trough. The vertical distribution and diversity of bacteria in the core was investigated through ten sub-sampling with an interval of 1 m using bacterial 16 S rRNA gene as a phylogenetic bio-marker. Eighteen phylogenetic groups were identified from 16 S rRNA gene clone libraries. The dominant bacterial groups were JS1, Planctomycetes and Chloroflexi, which accounted for 30.6%, 16.6%, and 15.6% of bacterial clones in the libraries, respectively. In order to reveal the relationship between biotic and abiotic data, a nonmetric multidimensional scaling analysis was performed. The result revealed that the δ15N, δ13C, total organic carbon and total organic nitrogen possibly influenced the bacterial community structure. This study expanded our knowledge of the biogeochemical cycling in the Xisha Trough sediment.

关键词： Xisha Trough bacterial diversity 16S rRNA gene

The bacterial diversity and community composition altered in the oxygen minimum zone of the Tropical Western Pacific Ocean

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Journal of Oceanology and Limnology 2021年第5期39卷 1690-1704页

作者： Qiqi SUN Jinming SONG Xuegang LI Huamao YUAN Qidong WANG Shandong Peanut Research Institute Qingdao 266100China Key Laboratory of Marine Ecology and Environmental Sciences Institute ofOceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot NationalLaboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao266071China

The oxygen minimum zones(OMZs)are globally expanding,yet the variation pattern of microbial communities related to dissolved oxygen levels remain *** variability of bacterial diversity and community composition(repre sented by 16 S rRNA)of six stations was investigated within the water column in the seamount area of Tropical Western Pacific Ocean(TWPO)in May *** seawater has dissolved oxygen(DO)concentration of 3.01-6.68 mg/L and the core of the oxygen minimum zones was located between the depths of 650 m and 1750 *** bacterial alpha-diversity showed unimodal pattern with the decreasing DO with depths and peaked in the upper oxycline(UO)of *** bacterial community structure of the mixed layer(ML)and the bottom layer clustered and separated from each other,while those of UO and the OMZ core(OM)clustered and ***,bacterial community composition transitioned from being Alphaproteobacteria and Gammaproteobacteria-dominant in ML to being Gammaproteobacteria and Nitrososphaeria/Deltaproteobacteria-dominant in UO and OM,and then changed to being Clostridia and unidentified Actinobacteria-dominant in the bottom ***,both bacterial alpha-diversity and the abundant classes fitted varying sectioned functions with *** DO solely explained 40.37%of the variation of bacterial community composition among layers(Pbacterial diversity and community composition,and help to clarify the potential responses of microbes and their involved biogeochemical processes to the expansion and intensification of OMZs.

关键词： bacterial diversity community composition oxygen minimum zones(OMZs) dissolved oxygen Tropical Western Pacific Ocean(TWPO)

The bacterial diversity in surface sediment from the South China Sea

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Acta Oceanologica Sinica 2010年第4期29卷 98-105页

作者： WANG Guanghua DONG Junde LI Xiang SUN Huimin Key Laboratory of Marine Bio-resources Sustainable Utilization South China Sea Institute of Oceanology Chinese Academy of Science Guangzhou 510301 China Graduate University of Chinese Academy of Science Beijing 100049 China

16S rDNA sequencing results from this study and literatures demonstrate that sediment bacteria in the South China Sea （SCS） were very diverse,which contained 22 of the 24 phyla of bacteria investigated from marine sediment,however,it was very imbalance among *** bacterial diversity from 15 samples which covered a wide range of sediment types from 20 to 3 888 m in depth was studied in DGGE （denature gradient gel electrophoresis） in this *** DGGE results indicate that both sediment bacterial diversity and diversity difference among stations were *** representative and differential fingerprints among samples were recovered and sequenced,phylogenetic analysis indicates that they may belong to Proteobacteria （α-,β-,γ-,δ-,ε-）,Planctomycetes,Firmicutes,Chloroflexi,Acidobacteria,Actinobacteria,Nitrospirae,Gemmatimonadetes,candidate division WS3 and so on,of which,Gemmatimonadetes and candidate division WS3 bacteria were first detected in SCS *** study also shows that bacterial diversity analysis based on DGGE was more potential than traditional 16S rDNA clone library in multiple sample analysis.

关键词： bacterial diversity DGGE marine sediment South China Sea

Different Decaying Wood Effects on bacterial diversity:Insights from Molecular Methods

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Phyton-International Journal of Experimental Botany 2021年第1期90卷 207-222页

作者： Mu Peng Yanli Jing Qiuyu Wang Shaopeng Yan College of Life Sciences Northeast Forestry UniversityHarbin150040China State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin150040China

Decaying wood is a novel key factor required for biodiversity and function of a forest,as it provides a good account of substrate and habitats for various ***,the bacterial diversity in decaying wood of Betula platyphylla was discussed through high throughput *** results showed that most of the obtained sequences belonged to the phyla Firmicutes,Proteobacteria,Bacteroidetes,Actinobacteria,Acidobacteria and *** community compositions in samples with higher moisture content were obviously different than that with lower content,which could be reflected by richness estimators,diversity indices,and cluster and heatmap *** three networks were non-random and possessed topological features of complex systems such as small-world and modularity ***,these networks exhibited distinct topological features,indicating the potential ability of extensive cooperative and competitive interactions in the decayed wood *** analysis showed that most bacterial phyla were mainly distributed in highermoisture *** obtained data will increase the knowledge of the complex bacterial diversity associated with dead wood,and lay a foundation for the bioconversion technology of plant cell walls using bacteria.

关键词： Decaying wood Betula platyphylla bacterial diversity moisture content network analysis

bacterial diversity in Arctic marine sediment determined by culture-dependent and-independent approaches

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Advances in Polar Science 2014年第1期25卷 46-53页

作者： LIN Xuezheng WANG Zhen CHE Shuai SONG Weizhi YI Dan Key Laboratory of Marine Bioactive Substances the First Institute of OceanographySOA

bacterial diversity in surface sediment from the Arctic Ocean was investigated by culture-dependent and-independent approaches. Conventional culture-dependent techniques revealed 11 strains based on their distinct morphological characteristics on marine Zobell 2216E agar plates. Phylogenetic analysis showed that these isolates belonged to three major lineages of the Bacteria,γ-proteobacteria, Bacteroidetes and Actinobacteria, and that they included 10 genera. Most isolates were psychrotrophic, and NaCl was not necessary for their growth. Furthermore, they exhibited activity of at least one extracellular hydrolytic enzyme at 4＆#176;C and had various abilities to assimilate carbon sources. A total of 67 phylotypes were detected among 142 clones based on the 16S rRNA library of the total community DNA and grouped into nine major lineages of bacteria. Phylotypes afifliated withγ-,δ-andε-proteobacteria accounted for 36.7%, 21.8%and 16.9%of the total clones, respectively. The rest of the clones belonged to Bacteroidetes,α-proteobacteria, Actinobacteria, Fusobacteria, Nitrospirae and an unclassiifed group.

关键词： Arctic marine sediment bacterial diversity culture-dependent culture-independent

Effects of Continuous Plastic Film Mulching on Soil bacterial diversity, Organic Matter and Rice Water Use Efficiency

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Journal of Geoscience and Environment Protection 2016年第4期4卷 1-6页

作者： Meiyan Wu Ruochao Hao Lianghuan Wu Engineering Research Center of Wetland Agriculture in the Middle Reaches of the Yangtze River Ministry of Education Hubei Provincial Key Discipline of Crop Science Yangtze University Jingzhou China Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition College of Environmental and Resource Sciences Zhejiang University Hangzhou China

Two field experiments were conducted to study the effects of 6-year plastic film mulching on bacterial diversity, organic matter of paddy soil and water use efficiency on different soils with great environmental variabilities in Zhejiang Province, China, under non-flooding condition. The experiment started in 2001 at two sites with one rice crop annually. Three treatments included plastic film mulching with no flooding (PM), no plastic film mulching and no flooding (UM), and traditional flooding management (TF). Soil samples were collected and analyzed for bacterial diversity by DGGE and organic matter content, and water use efficiency (WUE) was calculated. The results showed that PM treatment favored the development of a more total bacterial community compared with TF management, the total number of bands was 33.3, 31.7 at tiller stage and heading stage (p < 0.05*). Hence, organic matter content was decreased by 36.7% and 51.4% under PM at two sites. PM also produced similar rice grain yield as TF at Duntou site and Dingqiao site, the average was 7924 kg?ha?1 and 7015 kg?ha?1 for PM and 8150 kg?ha?1 and 6990 kg?ha?1 for TF, respectively. Compared to TF, WUE and irrigation water use efficiency were increased significantly by 70.2% - 80.4% and 273.7% - 1300.0% for PM. It is essential to develop the water-saving agriculture.

关键词： Rice (Oryza sativa L.) Water Use Efficiency Water-Saving Agriculture bacterial diversity

Cultivable bacterial diversity of the East China Sea

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International Journal of Technology Management 2013年第5期 101-104页

作者： Meng Fan-Xu Xu Xue-Wei Wu Yue-Hong Wu Min Wang Chun-Sheng Second Institute of Oceanography State Oceanic Administration Hangzhou 310012 China Laboratory of Marine Ecosystem and Biogeochemistry State Oceanic Administration Hangzhou 310012 China College of Life Sciences Zhejiang University Hangzhou 310058 China

bacterial diversity of 14 sites of the East China Sea was investigated by culture-dependent methods. The impact of human activities on marine bacteria was primarily studied and characteristics of bacteria communities in different areas were analyzed. A total of 396 strains were obtained. These strains belong to 4 phyla, 9 classes and 146 species according to 16S rDNA sequences alignment. For 32 strains, the 16S rDNA sequences similarities between isolated strains and their most closely related species were lower than 98%. The result indicated that there are abundant microbial diversity and a large number of unknown microbial resources in the East China Sea. Isolated strains were dominated byy-proteobacteria （64%）, ct-proteobacteria （18%） and Firmicutes （15%）. Actinobacteria and Bacteroidetes were less than 3%. Microbial community composition, diversity and abundance among areas with varies distances from land were different. The far the regions from the land, the lower the Shannon index （H＇） and the Margalef index （DMg） values were.

关键词： the East China Sea bacterial diversity Cultivable