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isolation of New Strains of bacteria Able to Synthesize 1,3-Propanediol from Glycerol

Advances in Microbiology 2013年第2期3卷 171-180页

作者： Daria Szymanowska-Powalowska Agnieszka Drozdzyńska Natalia Remszel Department of Biotechnology and Food Microbiology Poznań University of Life Sciences Poznan Poland

The natural environment is inhabited by many species that exhibit very specific metabolic activities that may find industrial applications. The aim of the study was to select non-pathogenic cultures of bacteria of the genus Clostridium and lactic acid bacteria able to convert glycerol into 1,3-propanediol (1,3-PD). Another aim of this study was to identify the isolates that best produced 1,3-propanediol both from pure and crude glycerol. The most efficient strains identified (Cl. butyricum) were analysed on a bioreactor scale. The aim was to determine temperature conditions on the efficiency and duration of 1,3-PD synthesis. The species Clostridium were identified using amplification of the 16S rRNA coding sequence. A total of 123 isolates (of the genus Clostridium and lactic acid bacteria) were isolated;a vast majority of these were able to synthesize 1,3-PD. The best results were obtained for Cl. butyricum strain DSP1, which was isolated from the rumen of a cow fed with glycerol. The strain efficiency using pure glycerol on bioreactor scale 0.65 mol/mol of glycerol at a temperature of 38℃ and a constant pH of 7.0.

关键词： Clostridium spp. bacteria isolation 1 3-Propanediol Glycerol Conversion Microflora of Natural Environment

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Identification and Susceptibility Test of Ralstonia solanacearum Isolated from White Burley

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Plant Diseases and Pests 2011年第5期2卷 61-64页

作者：李洪严占勇杨水英吴卫玲王晗李振轮西南大学资源环境学院重庆400716 四川省烟草公司达州市公司四川达州635006 西南大学植物保护学院重庆400716

[ Objective ] The paper was to isolate and identify Ralstonia solanacearum from white burley, and determine its susceptibility to 6 fungicides. [ Mcth- od] Using the combination method of semiselective medium （PCCG） and apolymerase chain reaction （PCR） technique, R. solanacearum in stalk of white burley from Dazhou City in Sichnan Province was isolated, and its biochemical type was identified. Through susceptibility test, the susceptibility of R. solanacearum to bismerthiazol, ethylicin, streptomycin, lime sulfur, 47% polylysine, 99% kojic acid was studied in laboratory. [Result] A total of 23 strains OfR. solanacearum were isolated, all belonging to biochemical type Ill. R. solanacearum obtained in the test were more susceptible to ethylicin, streptomycin and bismerthiazol, and ethylicin had good control effect against R. solanacearum with ECso of 0.086 ml/L. [ Conclusion ] The study provide theoretical basis for control of R. solanaceanon in white burley.

关键词： Ralstonia solanacearum bacteria isolation Identification PCCG Susceptibility test China

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