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Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by bacillus tequilensis g9

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Waste Disposal and Sustainable Energy 2019年第3期1卷 213-226页

作者： Mudasir A.Dar Kiran D.Pawar Jyoti M.Chintalchere Radhakrishna S.Pandit Department of Zoology Savitribai Phule Pune UniversityGaneshkhindPuneMaharashtra 411007India School of Nanoscience and Biotechnology Shivaji UniversityVidyanagarKolhapurMaharashtra 416004India

The ever increasing energy demands of modern civilization and rapidly dwindling fossil fuels point towards a renewable substitute like ***,higher costs associated with biofuel productions is the major bottleneck for its *** present study demonstrates the use of a statistical approach called response surface methodology(RSM)to investigate the optimum parameters for maximum production of cellulase by bacillus tequilensis *** Plackett-Bur-man design(PB)of the RSM analysis indicated grass straw(gS)concentration,pH,FeSO4,inoculum,MgSO4,incubation period and NH4Cl as significant variables that influence the cellulase ***,to propose the best medium for the maximum production of cellulase by *** g9,the most influential parameters,namely concentrations of gS as substrate,FeSO4,pH,inoculum size,*** fine-tuned by central composite design(CCD)involving four factors and five *** CCD analysis demonstrated 8%substrate concentration,1.5%of inoculum along with 10 ppm FeSO4 and a pH of 5.5 in media as optimum conditions for highest enzyme *** field emission scanning electron microscopic analysis of the treated gS showed structural alterations depicting significant deconstruction caused by *** *** yield of the partially purified cellulase proteins were found to be 21%revealing molecular mass between 30 and 97 *** enhanced cellulase production by *** g9 demonstrated in our study brands its applications in many industrial processes like biorefinery,biofuels,etc.

关键词： bacillus tequilensis g9 Cellulase production Plackett-Burman design Central composite design RSM Saccharification

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