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Study on enzymatic hydrolysis of soybean β-conglycinin using alkaline protease from bacillus subtilis accc 01746 and antigenicity of its hydrolysates

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Grain & Oil Science and Technology 2021年第1期4卷 18-25页

作者： Haicheng Yin Xinrui Zhang Jin Huang College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

Due to its beneficial health effects,the use of soybean protein has shown a continuous increase,but concerns regarding the allergenicity of soybean antigenic protein have also *** study aimed to evaluate the hydrolytic effects of a non-commercial alkaline protease isolated from the bacillus subtilis accc 01746 on soybeanβ-conglycinin and the allergenicity of its *** protease of the strain was separated by precipitation method of organic solvents,and theβ-conglycinin was separated by alkali-solution and acid-isolation and purified by use of gel *** the degree of hydrolysis(DH)and inhibition rate as evaluation indexes,the enzymatic hydrolysis parameters ofβ-conglycinin was optimized by single factor and L_(9)(3^(4))orthogonal tests,so as to explore the effect of the protease on the hydrolysis degree and the antigenicity ofβ-conglycinin *** results showed that the native enzyme existed as an 18.3 kDa monomer with a 430 U/g maximum *** purity ofβ-conglycinin was 84.8%.The single-factor test results showed that DH showed the oppostie trendency with the inhibition rate,and the increase of protein concentration causedmonotone increasing and monotone decreasing of the inhibition rate and the DH,and the optimal protein concentration was 30 mg/*** optimization results showed that pH had the largest impacts on both DH and the inhibition rate,followed by enzyme dosage,hydrolysis temperature and hydrolysis *** the optimum hydrolysis conditions of protein concentration 30mg/mL,enzymedosage0.7%,hydrolysis time40min,temperature 55°C and pH8.5,the DH reached the highest of 76.28%,and the inhibition rate was the lowest of 27.03%,which was reduced greatly compared with that before *** results suggested that alkaline protease appeared to show a relatively high effeciency in lowering soybean allergenicity,making it possible to produce low-allergenicity soybean protein.

关键词： Alkaline protease bacillus subtilis accc 01746 β-conglycinin Hydrolysis degree Antigenicity

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