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Detection of Acid Fast bacilli in Tuberculous Lymph Node Tissue and Touch Preparations—A Comparative Study

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Journal of Tuberculosis Research 2017年第3期5卷 168-177页

作者： Ghulam Rasool Muhammad Rashid Siraj Nadia Naseem Sameer Anjum Waqas Lateef A. H. Nagi Department of Morbid Anatomy & Histopathology University of Health Sciences Lahore Pakistan Department of Surgery Akhtar Saeed Medical & Dental College Lahore Pakistan

Background: The most common form of extrapulmonary tuberculosis is lymphadenitis. Lymphadenitis is considered mainly a disease of young children and has a peak age of onset at 20 to 40 years. Objectives: The purpose of this study was to detect and compare the presence of acid fast bacilli in lymph node biopsies and their touch preparations using Ziehl Neelsen and fluorescence staining. Materials and Methods: The lymph node biopsies and their touch preparations were prepared and stained with Haematoxylin and eosin to determine their morphology. Slides were stained with ZN staining and fluorescent staining technique using Auramine O and Rohdamine B to demonstrate the presence of acid fast bacilli in tissue and their touch preparations. Results: AFB was detected in 56% of biopsies and 54% of touch preparations. In addition in these cases we observed that fluorescent staining technique using Auramine O and Rohdamine B is superior to ZN stain because low magnification used in fluorescent staining technique makes it possible to scan the smear rapidly and detects the AFB as glowing spots even if it is present in small numbers. Conclusion: The results of AFB in tissue biopsies are in equivalent in their touch preparation.

关键词： Haematoxylin and Eosin Acid Fast bacilli Touch Preparation

Detection of 16S rRNA Methylase Genes in Gram-Negative bacilli Isolated from Hospitals in Changchun, China

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Advances in Infectious Diseases 2013年第4期3卷 290-294页

作者： Fan Zhao Hongyan Shi Jinghua Li Jiaqi Zhou Yanbo Sun Department of Orthopedics China-Japan Union Hospital of Jilin University Changchun China Department of Pathogen Biology Basic Medical College of Jilin University Changchun China Department of Pathogen Biology Basic Medical College of Jilin University Changchun China.

Methylation of 16S rRNA is an important mechanism of aminoglycoside resistance among gram-negative pathogens. In this report, 16S rRNA methylase genes were amplified using PCR among gram-negative bacillus isolates from hospitals in the Changchun area of China and 16S rRNA methylase genotypes (armA, rmtB, rmtA, rmtC, rmtD, and npmA) were identified by direct sequencing. Fifty of the isolates (43.1%) harbored 16S rRNA methylase genes. The common 16S rRNA methylase genes were armA and rmtB (12.1% and 31.0%, respectively), whereas the rmtA, rmtC, rmtD, and npmA genes were absent from the sample. It suggests that the predominant 16S rRNA methylase genes among gramnegative bacilli in the Changchun area are armA and rmtB.

关键词： 16S rRNA Methylases Gram-Negative bacilli

Metallo-β-lactamase producing nonfermentative gram-negative bacteria:An increasing clinical threat among hospitalized patients

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Asian Pacific Journal of Tropical Medicine 2012年第9期5卷 718-721页

作者： Varsha Gupta Shailpreet Sidhu Jagdish Chander Department of Microbiology CovtMedical College & HospitalSector-32ChandigarhIndia

Objective:To detect and evaluate the various methods for metallo-β-lactamases(MBL) production in Pseudomonas aeruginosa(***) and Acinetobacter ***:A total of 109 *** and 85 Acinetobacter species were screened for imipenem resistance by Kirby- Bauer disc diffusion *** of MBL production was(lone by imipenem-EDTA combined disc test,double disc synerygy test(DDST) and imipenem-EDTA MBL E ***: A total of 63(57.8%) strains of *** and 46(54.1%) strains of Acinetobacter *** found to be resistant to *** the 63 imipenem resistant *** tested for MBL production.44(69.89;) were found to be positive and among 46 imipenem resistant Acinetobacter. 19(41.3%) were shown to be the MBL ***:Imipenem-EDTA combined disc test and MBL E test are equally effective for MBL detection in both *** and Acinetobacter spp.,but given the cost-constraints,combined disc can be used as a convenient screening method in the clinical microbiology laboratory.

关键词： Carbapenem-imipenem Metallo-β-lactamases Non fermentative gram-negative bacilli

Poncet's disease: An unusual presentation of tuberculosis in a diabetic lady

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World Journal of Clinical Cases 2015年第4期3卷 385-388页

作者： Partha Pratim Chakraborty Sayantan Ray Chitra Selvan Rana Bhattacharjee Sanjay Kumar Mandal Department of Endocrinology IPGMER and SSKM Hospital Department of General Medicine Medical College and Hospital

Authors describe a 53-year-old woman who presentedto their diabetes clinic with a three week history of multiple painful and swollen joints. She had been diagnosed with type 2 diabetes 5 years back. On examination, both knee joints and left ankle were swollen. A soft tissue swelling appeared over the medial end of the left clavicle few days later. Rheumatoid arthritis, collagen vascular diseases and other common causes of polyarthritis were ruled out by appropriate investigations. Non steroidal anti-inflammatory drugs failed to give satisfactory pain relief and the arthritis persisted. Conventional cultures of synovial fluid samples including cultures for tuberculosis were negative. Computed tomography showed a space occupying lesion involving the left sternoclavicular joint. Fine needle aspiration from the lesion was performed and acidfast bacilli were demonstrated in the smear using ZiehlNeelsen stain. The explanation of her arthritis was therefore tuberculous arthritis in left sternoclavicular joint and reactive arthritis in the rest of the joints. A diagnosis of Poncet's disease was considered in her case. We treated her with standard anti-tuberculosis drugs and the arthritis resolved within a few days. She remained symptom-free at her 2 years' follow-up.

关键词： Poncet’s disease Diabetes Tuberculous arthritis Reactive arthritis Acid-fast bacilli

Tuberculosis:an experience from Mycobacterium smears and culture analysis

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Asian Pacific Journal of Tropical Medicine 2009年第2期2卷 47-53页

作者： Zeehaida M Siti Asma H Siti Hawa H Zaidah AR Norbanee TH Department of Medical Microbiology and Parasitology School of Medical SciencesUniversiti Sains MalaysiaKubang KerianKelantanMalaysia

Objective:Simple tests like direct smear of the acid fast bacilli(AFB) and Mycobacterium culture could assist the diagnosis of *** study is aimed at reviewing the outcome of smears,culture results and contamination rate among specimens requested for AFB smear and Mycobacterium ***:Retrospective laboratory data analysis requesting for Mycobacterium culture from January 2005 till December 2006 was done in a tertiary teaching hospital of Universiti Sains Malaysia,Kubang Kerian,Kelantan,***: Four hundred and sixty seven(36.6%) isolates grew from 1 277 *** these isolates,314 (67.2%) grew Mycobacterium tuberculosis,23(4.9%) grew Mycobacterium other than tuberculosis and 38 (8.1%) grew *** the *** cultures,165(52.5%) had growth of more than 100 confluent colonies,whereas 39 cultures(12.4%) had growth of less than 19 *** smear for AFB among smear positive cases showed presence of more than 50 bacilli/line in 231(49.5%) cases and smear negative cases accounted for 63(13.5%).Among smear positive cases,291(94.5%) cultures grew Mycobacterium species and another 17(5.5%) cultures grew *** smear negative cases,32(62.7%) cultures grew Mycobacterium species and 19(37.3%) cultures grew ***:The results from data analysis of the Mycobacterium cultures should be critically utilized in order to review the laboratory performance and to improve its services in the *** of the data is also useful to the administrators of the hospital in terms of estimating the risk of occupational hazard faced by the health care workers.

关键词： Tuberculosis Mycobacterium tuberculosis Culture Acid fast bacilli Smear Contamination

A middle-aged lady with a pyogenic liver abscess caused by Clostridium perfringens

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World Journal of Hepatology 2012年第8期4卷 252-255页

作者： Siu-Tong Law Ming Kai Lee Division of Gastroenterology and Hepatology Department of Medicine and GeriatricsTuen Mun HospitalTuen MunHong KongChina

The pyogenic liver abscess caused by Clostridium perfringens (C. perfringens ) is a rare, but rapidly fatal infection. It is usually associated with malignancy and immunosuppression. We report the case of 50-year-old lady with the secondary liver metastases from rectal cancer presented with fever and epigastric pain. The identification of Grampositive bacilli septicaemia, the presence of gas-forming liver abscess and massive intravascular hemolysis should lead to the suspicion of C. perfringens infection. Here we review twenty cases published since 1990 and their clinical features are discussed. The importance of "an aggressive treatment policy" with multidisciplinary team approach is emphasized.

关键词： Pyogenic liver abscess Clostridium perfringens Infected hepatic metastases Liver abscess Gram-positive bacilli septicaemia

Serratia marcescens and other non-AACEK GNB endocarditis: A case report and review of literature

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World Journal of Clinical Infectious Diseases 2019年第3期9卷 23-30页

作者： Helena Mertes Marielle Morissens Bhavna Mahadeb Evelyne Maillart Anthony Moreau Philippe Clevenbergh Infectious Disease Department Ziekenhuis Netwerk Antwerpen MiddelheimAntwerpen 2020Belgium Cardiology Department Brugmann University HospitalBrussels 1020Belgium Microbiology Department Brugmann University HospitalBrussels 1020Belgium Infectious Diseases Department Brugmann University HospitalBrussels 1020Belgium Intensive Care Unit Erasmus University HospitalIntensive Care UnitBrussels 1070Belgium

BACKGROUND Non-Aggregatibacter aphrophilus,Aggregatibacter actinomycetemcomitans,Cardiobacterium hominis,Eikenella corrodens,Kingella spp.(non-AACEK)gramnegative bacilli(GNBs)are an infrequent and challenging cause of endocarditis associated previously with mainly intravenous drug ***,this pathology has increasingly become a healthcare-associated *** guidelines do not clearly define the management of non-AACEK GNB endocarditis due to a lack of prospective *** review characteristics,outcomes and treatment of non-AACEK GNB endocarditis,in particular Serratia marcescens *** SUMMARY We describe the case report of a 46-year-old man who presented to the emergency department with high-grade fever and a purulent exudate on an intracardiac device *** marcescens mitral valve endocarditis as a consequence of complicated generator pocket infection was *** patient was treated with complete device removal and a long course of broadspectrum antibiotics for 6 wk after surgery with intravenous piperacillintazobactam and ciprofloxacin,which was later switched to oral ciprofloxacin and *** patient had complete resolution of symptoms and inflammatory parameters at the end of the treatment and at *** Long-term dual-antibiotic therapy containing a beta-lactam is indicated for most non-AACEK GNB endocarditis, whereas valve surgery may not be necessary inall patients.

关键词： Non-AACEK gram-negative bacilli endocarditis Serratia marcescens Healthcare-associated Intravenous drug use Case report Dual-antibiotic therapy

Status of ESBL Producing Bacteria Isolated from Skin Wound at a Tertiary Care Hospital in Bangladesh

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Advances in Infectious Diseases 2015年第4期5卷 174-179页

作者： Taslima Yasmin Md. Abdullah Yusuf Mohammed Abdun Nur Sayam Rezwana Haque Golam Mowla Department of Microbiology North Bengal Medical College Sirajgonj Bangladesh Department of Microbiology National Institute of Neurosciences & Hospital Dhaka Bangladesh Narayngonj General Hospital Narayngonj Bangladesh Department of Microbiology Islami Bank Medical College Rajshahi Bangladesh Department of Community Medicine Mymensingh Medical College Mymensingh Bangladesh

Background: ESBL producing bacteria are increasing with an alarming rate with a wide range of infections. Objective: The purpose of the present study was to see the status of ESBL producing bacteria isolated from skin wounds. Methodology: This cross sectional study was conducted in the Department of Microbiology at Mymensingh Medical College, Bangladesh from January 2011 to June 2011 for a period of 6 months. All the patients, at any age with both sexes presented with skin wound infection, were taken as study population. Wound swab was taken from all patients. Specimens were processed and bacteria were isolated and identified according to standard procedure. The ESBL status was confirmed by double disc diffusion test (DDDT) and minimum inhibitory concentration (MIC) by agar dilution method by standard procedure according to Clinical Laboratory Standard Institute (CLSI). Antimicrobial resistance was done by disc diffusion method. Result: A total number of 84 wound swabs were taken of which the most common ESBL producing bacteria were Esch. coli (61.5%),?Proteus species (78.3%) and Klebsiella species (88.9%). All the isolates were sensitive to imipenem and nitrofurantoin followed by amikacin (92.9%). Conclusion: In conclusion, ESBL producing E. coli is the most common bacteria causing skin wound infection followed by Proteus species with a reduced sensitivity towards antibiotics.

关键词： Extended Spectrum β-Lactamases Escherichia coli Klebsiella Species Gram Negative bacilli

The Increased Frequency of Carbapenem Resistant Non Fermenting Gram Negative Pathogens as Causes of Health Care Associated Infections in Adult Cancer Patients

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Journal of Cancer Therapy 2015年第10期6卷 881-888页

作者： Hadir A. El-Mahallawy Rasha M. Abdel Hamid Safaa Shawky Hassan Samah Radwan Magdy Saber Clinical Pathology Department National Cancer Institute Cairo University Giza Egypt Medical Oncology Department National Cancer Institute Cairo University Giza Egypt

Background and Aim: Multi drug resistant Non fermenting gram negative bacilli (NFGNB) have emerged as a major cause of health-care associated infections especially in immunocompromised hosts. The aim of the study was to investigate the prevalence of NFGNB as a cause of health-care associated infections (HAI) in cancer patients and determine their resistance pattern. Patients and Methods: During the study period, 158 NFGNB isolates were collected. Microscan Walk Away 9 was used for identification and testing for the metallo-β-lactamases (MBLs) was done by Imipenem-EDTA combined disk synergy test (CDST-IPM). Results: NFGNB represented 29.0% of infections caused by gram negative organisms. Carbapenem resistance, the multi-drug resistant (MDR) phenotype, and MBL production were documented in 70%, 63%, and 59% of NFGNB isolates, respectively. MDR-NFGNB rates were significantly higher among hospitalized patients, medical department and those with longer duration of hospital stay (p = 0.034, 0.026, 0.019;respectively) than non MDR-NFGNB. Conclusion: A high level of carbapenem and multi-drug resistance were detected among the non-fermenter pathogens isolated from hospitalized cases and were more frequently encountered in high risk adult cancer patients requiring longer duration of hospitalization. The MDR-NFGNB are constituting important causes of health-care associated infections in cancer patients.

关键词： Multi Drug Resistant Organisms (MDRO) Non-Fermenting Gram Negative bacilli (NF-GNB) Metallo-β-Lactamases (MBL) Surgical Site Infections (SSI)