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bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics

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Journal of Animal Science and Biotechnology 2020年第1期11卷 95-110页

作者： Diana Luise Micol Bertocchi Vincenzo Motta Chiara Salvarani Paolo Bosi Andrea Luppi Flaminia Fanelli Maurizio Mazzoni Ivonne Archetti Giuseppe Maiorano Bea K.K.Nielsen Paolo Trevisi Department of Agricultural and Food Sciences University of BolognaViale G.Fanin 4640127 BolognaItaly Department of Agricultural Environmental and Food SciencesUniversity of MoliseVia F.De SanctisCampobassoItaly Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna Bruno Ubertini V.Bianchi 925124 BresciaItaly Endocrinology Unit and Center for Applied Biomedical Research Department of Medical and Surgical SciencesUniversity of Bologna–S.Orsola-Malpighi Hospitalvia Massarenti 940138 BolognaItaly Department of Veterinary Medical Sciences University of BolognaVia.Tolara di Sopra 5040064 Ozzano EmiliaItaly Chr.Hansen A/S Boege Allé10-122970 HoersholmDenmark

Background:Probiosis is considered a potential strategy to reduce antibiotics use and prevent postweaning diarrhea(PWD).This study investigated the effect of bacillus amyloliquefaciens DSM25840 or bacillus subtilis DSM25841 supplementation on growth,health,immunity,intestinal functionality and microbial profile of post-weaning pigs after enterotoxigenic ***(ETEC)F4 ***:Sixty-four post-weaning piglets(7748 g±643 g)were randomly al ocated to four groups:control basal diet(CO);CO+1.28×10~6 CFU/g of ***(BAA);CO+1.28×10~6 CFU/g feed of ***(BAS);CO+1 g colistin/kg of feed(AB).At day(d)7,animals were chal enged with 10~5 CFU/m L of ETEC F4 ac O149 and then fol owed for fecal score and performance until d *** was col ected at d 6,d 12 and d 21 for immunoglobulins,at d 8 for acute phase proteins,at d 8 and d 21 for metabolomics *** was sampled for morphometry,quantification of apoptosis,cel proliferation,neutral and acid mucine and Ig A secretory cel s,and microarray analysis at d *** and cecum contents were col ected for microbiota at d ***:AB and BAS reduced the fecal score impairment compared to CO(Pbacil us groups and *** improved BW at d 7,d 14 and d 21,ADWG ADFI and G:F from d 0 to d 7(P<0.05).At d 8,CO had higher plasma arginine,lysine,ornithine,glycine,serine and threonine than other groups,and higher haptoglobin than AB(P<0.05).At d 21,CO had lower blood glycine,glutamine and Ig A than ***,cel s apoptosis and mucins did not *** and AB increased the vil us mitotic *** profile of BAS and AB were more similar than *** sets related to adaptive immune response were enriched in BAA,BAS and *** had enriched gene set for nuclear structure and RNA *** had a trend of higher Enterobacteriaceae in cecum than the oth

关键词： bacil us amyloliquefaciens bacil us subtilis Diarrhea ETEC Gut microbiota Post-weaning Transcriptomics

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Characterization of two alkyl hydroperoxide reductase C homologs alkyl hydroperoxide reductase C_H1 and alkyl hydroperoxide reductase C_H2 in bacillus subtilis

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World Journal of Biological Chemistry 2015年第3期6卷 249-264页

作者： Mee-Kyung Cha Yoo-Jeen Bae Kyu-Jeong Kim Byung-Joon Park Il-Han Kim Department of Life Science and Technology Daeduk Valley Campus Paichai University

AIM: To identify alkyl hydroperoxide reductase subunit C(AhpC) homologs in bacillus subtilis(B. subtilis) and to characterize their structural and biochemical properties. AhpC is responsible for the detoxification of reactive oxygen species in ***: Two AhpC homologs(AhpC_H1 and AhpC_H2) were identified by searching the B. subtilis database; these were then cloned and expressed in Escherichia coli. AhpC mutants carrying substitutions of catalytically important Cys residues(C37S, C47 S, C166 S, C37/47 S, C37/166 S, C47/166 S, and C37/47/166 S for AhpC_H1; C52 S, C169 S, and C52/169 S for AhpC_H2) were obtained by site-directed mutagenesis and purified, and their structure-function relationship was analyzed. The B. subtilis ahp C genes were disrupted by the short flanking homology method, and the phenotypes of the resulting AhpC-deficient bacteria were ***: Comparative characterization of AhpC homologs indicates that AhpC_H1 contains an extra C37, which forms a disulfide bond with the peroxidatic C47, and behaves like an atypical 2-Cys AhpC, while AhpC_H2 functions like a typical 2-Cys AhpC. Tryptic digestion analysis demonstrated the presence of intramolecular Cys37-Cys47 linkage, which could be reduced by thioredoxin, resulting in the association of the dimer into higher-molecular-mass complexes. Peroxidase activity analysis of Cys→Ser mutants indicated that three Cys residues were involved in the catalysis. AhpC_H1 was resistant to inactivation by peroxide substrates, but had lower activity at physiological H2O2 concentrations compared to AhpC_H2, suggesting that in B. subtilis, the enzymes may be physiologically functional at different substrate concentrations. The exposure to organic peroxides induced AhpC_H1 expression, while AhpC_H1-deficient mutants exhibited growth retardation in the stationary phase, suggesting the role of AhpC_H1 as an antioxidant scavenger of lipid hydroperoxides and a stress-response factor in B. subtilis. CONCLUS

关键词： Cysteine-dependent peroxidase Thioredoxin Thiol peroxidase Peroxiredoxin Alkyl hydroperoxide Ortholog bacil us subtilis Oxidative stress

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