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Photochromic kinetic spectra and intermediates of br-d96n

Science China(Physics,Mechanics & Astronomy) 2003年第1期46卷 1-7页

作者：郑媛姚保利王英利雷铭陈国夫 norbert Hampp State Key Laboratory of Transient Optics Technology Xi'an Institute of Optics and Precision MechanicsChinese Academy of SciencesXi'an 710068China Institute for Physical Chemistry University of MarburgD-35032 MarburgGermany

br-d96n is a kind of genetically site-specific mutant of bacteriorhodopsin (br) with obvious photochromic effect. Compared to the wild type br, the lifetime of M state of br-d96n is prolonged to several minutes so that the photochromic kinetics and the intermediates formation can be studied by the conventional spectra analysis. In the experiment, the absorption spectra of the sample at different time after light illumination are measured with spectrophotometer. By fitting and analyzing the variation of the spectra, we suppose there are three main states in the photochromic process, i.e. B state (light-adapted state), M state and d state (dark-adapted state). The absorption spectra of the B state, M state and d state are extracted from the experimental data based on this three-state model and the spectra at various time are fitted with the least square method. So, the variations of population percentages of the M state, B state and d state are obtained and the M state and B state lifetimes are estimated. In another way, from measuring the absorption dynamics at 407 nm and 568 nm, the M state and B state lifetimes are also obtained by two exponential data fitting, which give coincident results with those of the spectra analysis.

关键词： br-d96n, photochromic, thermal relaxation, absorption spectra.

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Experimental study on polarization holographic storage in genetic mutant br-d96n film

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Science China(Physics,Mechanics & Astronomy) 2004年第3期47卷 284-292页

作者： MEnKE neimule norbert HAMPP State Key Laboratory of Transient Optics Technology Xi'n Institute of Optics and Precision Mechanics Chinese Academy of Sciences Xi'n 710068 ChinaInstitute of Physical Chemistry University of Marburg D-35032 Marburg Germany

Recording with both parallel and orthogonal linearly polarized lights, polarization holographic storage in genetic mutant br-d96n film is reported with both transmission type geometry and reflection type geometry. Polarization properties of diffraction light and scattering light are discussed for two different cases, parallel polarization recording and orthogonal polarization recording. It shows that, compared with recording with parallel polarization lights, orthogonal polarization holography can separate the diffraction light from the scattering noise, therefore improving the signal-to-noise ratio. It also shows that, compared with reconstruction with reference light, reconstruction with phase conjugated wave of the reference light can improve the signal-to-noise ratio of the reconstructed diffraction image, and also the wave-front aberration of the object light introduced by irregular phase object in the optical pass-way can also be corrected effectively, which ensures that the reconstructed diffraction image has a better fidelity. The preliminary angle-multiplexed volume holographic storage multiplexed by transmission type geometry and reflection type geometry is demonstrated in the br-d96n film. Experiment shows that there is no cross-talk between the two pages of images except for some scattering noises.

关键词： polarization holography, br-d96n, photochromism, phase conjugation, photo-induced anisot-ropy.

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