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Fabrication of HRP/Bi_(2)WO_(6) photoenzyme-coupled artificial catalytic system for efficiently degrading bisphenol A

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Chinese Chemical Letters 2021年第6期32卷 2047-2051页

作者： Hongjun Dong Ning Song Ming Yan Huihui Wu Haibo Zhang Changchang Ma Yun Wang Advanced Chemical Engineering Laboratory of Green Materials and Energy of Jiangsu Province Institute of Green Chemistry and Chemical TechnologySchool of Chemistry and Chemical EngineeringJiangsu UniversityZhenjiang 212013China Department of Chemistry Dongguk UniversitySeoul 04620Republic of Korea

A novel photoenzyme-coupled artificial catalytic system is fabricated by immobilizing horseradish peroxidase(HRP)on the Bi_(2)WO_(6)hollow nanospheres via a facile electrostatic self-assembly process.The obtained Bi_(2)WO_(6)/HRP sample not only improves the visible light harvest ability but also promotes the high-efficiency separation of charge carriers.More importantly,the photogenerated electrons and produced H2O2 on Bi_(2)WO_(6)directly take part in redox cycle reactions of HRP to induce photoenzyme synergic catalytic effect.In consequence,the degradation activity of Bi_(2)WO_(6)/HRP is significantly improved relative to Bi_(2)WO_(6)and HRP for removing bisphenol A(bpa)under the visible light irradiation.This work launches a feasible design strategy for exploiting photoenzyme-coupled artificial catalytic system with special structure to degrade organic pollutants in water efficiently.

关键词： Photoenzyme-coupled Bi_(2)WO_(6)/HRP Synergistic catalytic effect bpa degradation Reaction mechanism

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Isolation and characterization of Acinetobacter sp. T32 capable of removal both nitrogen and bisphenol A

Isolation and characterization of Acinetobacter sp. T32 capa...

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“全球变化下的海洋与湖沼生态安全”学术交流会

作者： Weiwei Zhang Qiaoning Wang Chenghua Li Lingxin Chen School of Marine Sciences Ningbo University Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone Research (YIC) Chinese Academy of Sciences (CAS)

AIM: To identify and characterize bacterium that possessed both the nitrogen removal activity and estrogen removal activity. METHODS:Semi-quantitative ammonia detection to determine the NH4+-N removal activity of Acinetobacter sp. T32 was carried out using the kit purchased from Luheng Biotech. Com. The concentrations of NH4+-N, NO2–-N and NO3–-N were also detected to determine the nitrogen removal activity. In addition, bpa degradation was simultaneously monitored by using HPLC. A subunit of ammonia monooxygenase（AMO） of Acinetobacter sp. T32, amoAAc, was cloned and characterized for its structural domain and homology analysis, and its transcription in the mRNA level by using real-time reverse transcriptase PCR. RESULTS: Acinetobacter sp. T32 almost 100% removed NH4+ at concentrations below 100 mg/L and 98% of total inorganic nitrogen from the aqueous phase after inoculated for 48 h, with only a trace production of NO2– and NO3–. Acinetobacter sp. T32 could also degrade bpa using bpa as sole carbon source, but with a lower efficiency. mRNA level of amoAAc was 22-fold and 12-fold increase in the presence of NH4+ and bpa, respectively. 50 mg/L allylthiourea, the typical inhibitor of AmoA of autotrophic nitrifying bacteria, down-regulated the mRNA level of amoAAc. LD50 studies revealed that Acinetobacter sp. T32 was non-toxic to Japanese flounder, which suggested that Acinetobacter sp. T32 can be safely used in water quality enrichment. CONCLUSIONS: An Acinetobacter sp. T32 was characterized to possess high removal efficiency of both nitrogen and bpa. 60 mg/L NH4+-N and 1 μg/L bpa could be completely removed from the water sample. The gene encoding AmoAAc, the subunit AMO of Acinetobacter sp. T32, was cloned and characterized for the first time. AmoAAc contained the conserved AmoA domains and was significantly up-regulated in mRNA level in the presence of NH4+ and bpa, respectively. Allylthiourea partly interfere the function of AmoAAc during the process of bpa degradation, but not effective as the inhibitory effec

关键词： Acinetobacter sp.T32 Nitrogen removal bpa degradation Ammonia monooxygenase Toxicity

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