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Protective Effect of Buyang Huanwu Decoction on Cerebral Ischemia/Reperfusion Injury by Inhibiting Autophagy Based on AMPK/UIK1Signaling Pathway

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Journal of Integrative Medicine（双语） 2022年第2期11卷 9-28页

作者： Ruijuan Zhang Yanmeng Zhao Xiujuan Ma Jiabei Ye Yudong Shan Wentao Yu Xiaohong Zhou Weijuan Gao Hebei University of Chinese Medicine No.3Xingyuan RoadLuquan DistrictShijiazhuangHebei050200China

The aim of the paper is to explore whether AMPK/U1K1 signaling pathway is involved in regulating autophagy and alleviating cerebral ischemia/reperfusion(I/R)*** this basis,study the regulatory effect of Buyang Huanwu Decoction(BHD)on AMPK/U1K1 signaling pathway,aiming to clarify the molecular mechanism of BHD in alleviating cerebral I/R injury and the protective effect of BHD containing serum(bhds)on OGD/R *** research group adopted the following methods:Neurons cultured for 7 days in vitro were randomly divided into normal control group,model group(OGD/R),AMPK activator(AICAR)group(3 mmol/L),Buyang Huanwu Decoction medicated serum(bhds)group(3%),Buyang Huanwu Decoction medicated serum+AMPK activator(bhds+AICAR)*** protein expressions of p-AMPK,AMPK,p-UlKl,U1K1,Beclin-1 and LC3B in neurons were detected by Western blot,the fluorescence intensity and apoptosis rate of neuron autophagosome CYTO-ID staining were detected by flow cytometry,the fluorescence intensity and morphological changes of neuron LC3B were detected by immunofluorescence double labeling method,the morphological changes of neurons were observed by inverted microscope,and the survival rate of neurons was detected by CCK-8 *** OGD/R model and the optimal concentration of *** neurons in the model group were subjected to hypoxia and glucose deprivation for 1 h,2 h,3 h,4 h and reoxygenation and glucose deprivation for 24 *** survival rate of neurons and the release rate of 1DH to determine whether the model was successfully *** the results show that bhds group,the survival rate of OGD/R neurons was significantly increased,and the apoptosis rate was significantly *** them,3%bhds intervention at the same time of hypoxia glucose deprivation for 3 h and reoxygenation glucose for 24 h was the best *** bhds group,the levels of p-AMPK/AMPK,p-UlKl/UlKl,beclin-1,CYTO-ID and LC3B fluorescence intensity,and the transformation from LC3B-I to LC3B-I

关键词： bhds Drug containing serum Traditional Chinese medicine Signal pathway Cell damage

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