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Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and b.cillus cereus from Cows with Endometritis

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Agricultural Sciences in China 2011年第10期10卷 1624-1629页

作者： SUN Dong-b. WU Rui HE Xian-jing WANG Shuang LIN Yun-cheng HAN Xu WANG Yue-qiang GUOTing-ting WU Guo-jun YANG Ke-li College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University Daqing 163319 P.R.China

Staphylococcus aureus, Escherichia coli and b.cillus cereus are the major agents of cow endometritis in dairy cows. A multiplex PCR （SEb.mPCR） was estab.ished b.sed on the conserved genes of S. aureus, E. coli and b. cereus, and the detection limits were 103, 102 and 103 CFU mL-1, respectively. SEb.mPCR could not amplify genomic DNA of pathogenic b.cteria of other common b.vine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested b. SEb.mPCR. Of the samples, 23.95% had the three kinds of b.cteria detected, 17.15% had S. aureu and E. coli, 9.39% had E. coli and b. cereus, and 9.71% had S. aureus and b. cereus. The rates of infections with S. aureus, E. coli and b. cereus were 11.35, 16.18 and 9.06%, respectively. Therefore, SEb.mPCR has a potential as a diagnosis tool for endometritis in dairy cows.

关键词： b.vine endometritis multiplex PCR S. aureus E. coli b. cereus

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Association of Genotyping of b.cillus cereus with Clinical Features of Post-Traumatic Endophthalmitis

Association of Genotyping of Bacillus cereus with Clinical F...

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第十二届全国免疫学学术大会

作者： Zhu Gejing Hong Meng Lou Yongliang Zheng Meiqin The Eye Hospital of Wenzhou Medical University China Ministry of Education Key Lab.of Lab.ratory Medicine Wenzhou Medical University

b.cillus cereus is the second most frequent cause of post-traumatic b.cterial *** genotyping of *** associated with gastrointestinal infections has b.en reported,little is known ab.ut the *** clinical isolates associated with post-traumatic *** is largely due to the limited numb.r of clinical strains availab.e isolated from infected tissues of patients with post-traumatic *** this study,we report successful isolation of twenty-four *** strains from individual patients with different disease severity of post-traumatic *** analysis showed that all strains could b. categorized into three genotypes(GTⅠ,GTⅡ and GTⅢ) and the clinical score showed significant differences among these *** then further performed genotyping using the vrrA gene,etab.ished a vitro model of b.teroidal endophthalmitis to investigate the toxic effects of different *** clinical isolates on muller cells and evaluated possib.e correlation of genotype,virulence phenotype and the clinical features of ***-caused post-traumatic *** found that the disease of onset,final vision acuity and virulence phenotype were significantly different among the three *** results suggested that the vrrA gene may play a significant role in the pathogenesis of endophthalmitis,and genotyping of *** has the potential for predicting clinical manifestation and prognosis of *** the b.st of our knowledge,this is the first report of isolation of large numb.rs of clinical isolates of *** from patients with *** work sets the foundation for future investigation of the pathogenesis endophthalmitis caused b. *** infection.

关键词： b. cereus Post-Traumatic Endophthalmitis

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