检索结果-南通市图书馆

Molecular basis of CONSTANS oligomerization in FLOWERING LOCUS T activation

Journal of Integrative Plant biology 2022年第3期64卷 731-740页

作者： Xiaolin Zeng Xinchen Lv Rui Liu Hang He Shiqi Liang Lixian Chen Fan Zhang Liu Chen Yuehui He Jiamu Du Peking-Tsinghua Center for Life Sciences School of Advanced Agricultural SciencesPeking UniversityBeijing100871 China National Key Laboratory of Plant Molecular Genetics&Shanghai Center for Plant Stress biology Chinese Academy of Sciences Center for Excellence in Molecular Plant SciencesShanghai201602 China Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Institute of Plant and Food ScienceDepartment of BiologySchool of Life SciencesSouthern University of Science and TechnologyShenzhen518055 China Peking University Institute of Advanced Agricultural Sciences Weifang261000 China

The transcription factor CONSTANS(CO)integrates day-length information to induce the expression of florigen FLOWERING LOCUS T(FT)in Arabidopsis.We recently reported that the C-terminal CCT domain of CO forms a complex with NUCLEAR FACTOR-Yb/YC to recognize multiple cis-elements in the FT promoter,and the N-terminal tandem b-box domains form a homomultimeric assembly.However,the mechanism and biological function of CO multimerization remained unclear.Here,we report that CO takes on a head-to-tail oligomeric configuration via its b-boxes to mediate FT activation in long days.The crystal structure of b-boxesCOreveals a closely connected tandem b-box fold forming a continuous head-to-tail assembly through unique CDHH zinc fingers.Mutating the key residues involved in CO oligomerization resulted in a non-functional CO,as evidenced by the inability to rescue co mutants.by contrast,a transgene encoding a human p53-derived tetrameric peptide in place of the b-boxesCOrescued co mutant,emphasizing the essential role of bboxesCO-mediated oligomerization.Furthermore,we found that the four TGTG-bearing cis-elements in FT proximal promoter are required for FT activation in long days.Our results suggest that CO forms a multimer to bind to the four TGTG motifs in the FT promoter to mediate FT activation.

关键词： b-box domain CONSTANS crystal structure FLOWERING LOCUS T photoperiod

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：