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Removal of methyl orange from aqueous solution by azolla filicoloides:Synthesis of Fe_3O_4 nano-particles and its surface modification by the extracted pectin of azolla

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Chinese Chemical Letters 2011年第4期22卷 501-504页

作者： Roohan Rakhshaee Masoud Giahi Afshin Pourahmad Department of Chemistry Islamic Azad UniversityRasht Branch41335-3516RashtIran Department of Chemistry Islamic Azad UniversityLahijan Branch1616LahijanIran

The modified Fe3O4 nanoparticles with the extracted pectin from the cell wall of azolla filicoloides（FNEP） can remove methyl orange as a watersoluble azo dye from waste water better than azolla and the extracted pectin from azolla（EPA）,*** could be due to more crowding the main functional groups of uptake after binding pectin with *** studies showed that adsorption equilibrium constant（KL） and maximum adsorption capacities（Q（max）） were increased with decreasing temperature（exothermic）.The maximum uptake capacity（Q（max）） of dye by FNEP in a batch reactor was 0.533,0.498 and 0.446 mmol/g at 5,25 and 50℃,*** enthalpy change（△H） and entropy change（△S） were 15.31 kJ/mol and 0.02434 kJ/mol K,respectively.

关键词： Methyl orange uptake Modified Fe3O4 nano-particles Extracted pectin azolla

2,4-D Hyper Accumulation Induced Cellular Responses of azolla pinnata R. Br. to Sustain Herbicidal Stress

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Phyton-International Journal of Experimental Botany 2020年第4期89卷 999-1017页

作者： Arnab Kumar De Arijit Ghosh Debabrata Dolui Indraneel Saha Malay Kumar Adak Plant Physiology and Plant Molecular Biology Research Unit Department of BotanyUniversity of KalyaniWest BengalIndia

In the present experiment with ongoing concentration(0µM,100µM,250µM,500µM and 1000µM)of 2,4-D,the responses of azolla pinnata *** evaluated based on cellular ***,plants were significantly tolerated up to 1000µM of 2,4-D with its *** was accompanied by a steady decline of indole acetic acid(IAA)concentration in tissues with 78.8%over the *** bound H^(+)-ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator(KCl)and decreased within a range of 57.3 to 74.6%in response to inhibitor(Vanadate)*** regards to IAA metabolism,plants recorded a linear increase with wall bound oxidase activity up to maximum concentration of 2,*** variations were more moderated when wall bound IAA-oxidase recorded a linear increase proportionate to the 2,4-D *** was more extended with the presence of different isoforms of IAA-oxidase which was much more pronounced with distinct polymorphisms of expressed proteins,however,not independent to the 2,4-D *** like spermine,spermidine and putrescine(spm,spd and put)were not consistent in concentration with the dosages of 2,*** these,plants were induced to apoplastic NAD(P)H oxidase activity maximally by 1.6 folds under 500µM 2,4-D over ***,putrescine responded more or less consistently and recorded maximally 11.9 folds at 500µM 2,4-D as compared to the ***(P)H oxidase activity recorded maximally 1.6 folds against control and remain consistent throughout the concentrations of 2,*** along with APX were more linear in responses through the concentration of 2,4-D except CAT as compared to *** enzymatic antioxidative activity,peroxidases(GPX and APX)were overexpresed in a similar manner except for catalase with a non-significant *** stabilization of cellular redox,glutathione reductase attended maximum value by 2.45 folds at 1000µM evidenced with significant variations in protein polymo

关键词： azolla 2,4-D antioxidative enzymes comet assay HPLC

Poultry litter biochar application in combination with chemical fertilizer and azolla green manure improves rice grain yield and nitrogen use efficiency in paddy soil

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Biochar 2021年第4期3卷 591-602页

作者： Samuel Munyaka Kimani Putu Oki Bimantara Valensi Kautsar Keitaro Tawaraya Weiguo Cheng The United Graduate School of Agricultural Sciences Iwate UniversityMorioka 020-8550Japan Graduate School of Agricultural Sciences Yamagata University1-23Wakaba-machiTsuruokaYamagata 997-8555Japan

Poultry litter biochar is known to improve crop ***,its beneficial interactions with chemical fertilizer and/or organic manure on rice yield and nitrogen(N)use efficiency(NUE)are not well *** objective of this study was to co-apply poultry litter biochar(hereinafter biochar)and chemical fertilizer and/or azolla as organic manure(herein N fertilizer sources)to improve the productivity of rice and *** treatments-no amendments(control),chemical fertilizer(NPK),azolla as green manure(azolla),and NPK+azolla without and with biochar amendment-were evalu-ated in a pot *** rice plant growth components,yield,and NUE were *** to the treatments without biochar,co-application of biochar and N fertilizer sources significantly improved grain N uptake by 23.9%and NUE by 34.3-246.9%.These treatments also significantly improved rice growth components(5.6-18.2%)and grain yield(32.4%).Significant changes in soil properties including increases in pH,electrical conductivity(EC),total N,organic car-bon,and available phosphorus were observed following biochar *** for the soil pH and EC parameters,no significant synergistic interactions between biochar and N fertilizer sources were observed for any parameters in the present ***,compared to other treatments,the co-application of biochar and azolla offers a feasible approach to improve rice productivity and NUE and reduce chemical fertilizer use,thereby reducing agricultural pollution and production costs.

关键词： azolla N use efficiency Poultry litter biochar Rice paddy Synergistic effects

RE-ESTABLISHMENT OF SYMBIOSIS TO Anabaena-FREE azolla

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Science China Chemistry 1989年第5期32卷 551-559页

作者：林沧刘中柱郑德英唐龙飞渡边岩 Fujian Academy of Agricultural Sciences Fuzhou International Rice Research Institute Manila Philippines

Symbiosis to Anabaena-free azolla has been re-established by placing indusium containing Anabaena onto the Anabaena-free megasporocarps. Anabaena-free megasporocarps were obtained by removing the indusium and the apical membrane of the megaspore apparatus of normal azolla. The recovery of symbiosis in artificially reconstituted azolla-Anabaena association was confirmed using the scanning electron microscope, monoclonal antibody test (McAb-C16), and nitrogen fixation capacity （ARA）.Laboratory-grown Anabaena azollae cells inoculated subsequently to the decapitated megaspore apparatus were observed to have entered the leaf cavities of sporophyte, although some portions of Anabaena filaments were found outside the leaf cavity. Plants invaded by artificially inoculated Anabaena did not have sufficient N2-fixing activity to allow the growth in the N-free medium.

关键词： re-establishment of symbiosis incomplete symbiosis Anabaena-free azolla azolla.

Evaluation of Nitrogen and Phosphorus Wastes Produced by Nile Tilapia (Oreochromis niloticusL.) Fed azolla-Diets in Earthen Ponds

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Journal of Environmental Protection 2012年第6期3卷 502-507页

作者： Youssouf Abou Aliou Saidou Daouda Mama Emile D. Fiogbé Jean-Claude Micha Laboratory of Applied Hydrology Faculty of Sciences and Technics University of Abomey-Calavi Abomey-Calavi Republic of Benin Laboratory of Soil Sciences Department of Crop Production Faculty of Agricultural Sciences University of Abomey-Calavi Abomey-Calavi Republic of Benin Research Unit in Environmental and Evolutionary Biology FUNDP-University of Namur Namur Belgium Research Unit on Wetlands Department of Zoology and Genetic Faculty of Sciences and Technics University of Abomey-Calavi Abomey-Calavi Republic of Benin

Nitrogen (N) and phosphorus (P) wastes produced by Nile tilapia Oreochromis niloticus L. fed azolla, an aquatic atmospheric nitrogen fixing fern, was evaluated for 90 days in pond experiment. Six isonitrogenous (29.2% crude protein) and isoenergetic (16.9 Kj·g–1) diets A0, A10, A20, A30, A40 and A50, containing 0%, 10%, 20%, 30%, 40% and 50% of azolla meal (AM) respectively, as partial fishmeal (FM) substitutes, was provided to experimental fish. The azolla-free diet A0 served as a control. Fish specific growth rate (SGR) was higher with the control diet, the lower values being obtained in A50-fed fish (P azolla could be used in diet to sustain Nile tilapia growth and as “environmentally-friendly” ingredient to limit P loss, while providing N to the field, beneficially in tropical marshland pond where this nutrient is already limiting.

关键词： azolla Aquaculture Environment Fish Meal Replacement Oreochromis niloticus Nitrogen Phosphorus Nutrient Balance

Approximate Compositional Values and Tissue Fatty Acid Profiles of Nile Tilapia (Oreochromis niloticusL.) Fed azolla-Diets in Earthen Ponds

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Food and Nutrition Sciences 2011年第9期2卷 964-973页

作者： Youssouf Abou Emile Didier Fiogbé Yves Beckers Jean-Claude Micha 不详

The approximate general composition and the fatty acid profile of Nile tilapia fed azolla-diets in ponds were studied for 90 days. Six isonitrogenous (29.2% CP) and isoenergetic (16.9 kJ·g>sup>-1) diets were formulated to contain 0% (A0), 10% (A10), 20% (A>sub>20), 30% (A30), 40% (A40) and 50% (A50) of azolla meal (AM), as partial fish meal (FM) substitutes. Diet A0 without AM served as a control. Fish growth decreased as AM level exceeded 20% in diets (P 0.05). Crude lipid was significantly lower in fish fed A50 and significant differences were also found in crude ash (P 20, A30 and A50. High level of the fern reduces growth without negatively affecting fatty acid in fish. Fish PUFA, especially the (n - 3) fatty acids, are affected positively, even when fed 50% AM, which is good for the quality of the fish produced in regard to the benefits for the health of consumers.

关键词： azolla Filiculoides Fish Meal Replacement Oreochromis Niloticus Fatty Acid Natural Food Earthen Pond

Growth and fatty acid composition of Nile tilapia Oreochromis niloticusL. fed azolla-diets, in tanks and in earthen ponds: A comparative study

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Natural Science 2013年第1期5卷 77-83页

作者： Youssouf Abou Martin P. Aina Emile D. Fiogbé Jean-Claude Micha Laboratory of Water Sciences and Technics Polytechnic School of Abomey-Calavi University of Abomey-Calavi Abomey-Calavi Benin Research Unit in Environmental and Evolutionary Biology FUNDP-University of Namur Namur Belgium Research Unit on Wetlands Department of Zoology Faculty of Sciences and Technics University of Abomey-Calavi Abomey-Calavi Benin Research Unit on Wetlands Department of Zoology Faculty of Sciences and Technics University of Abomey-Calavi Abomey-Calavi Benin

Two 90-day experiments were conducted in the same period in tanks and in earthen ponds to study the effects of culture systems on growth and fatty acid (FA) of Nile tilapia (Oreochromis niloticus L.) fingerlings (mean weight: 16.3 g) fed azolla, as partial replacement for fish meal (FM). Diets were isonitrogenous (29.2% CP) and isoenergetic (16.9 kJ·g-1) containing 0% (A0), 10% (A10), 20% (A20), 30% (A30), 40% (A40) and 50% (A50) of azolla meal (AM) respectively. Diet A0, without AM, acted as a control. In both experiments, fish growth decreased as AM level increased in diets (P P 30-A50). Interestingly, those fish were rich in eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). Consequently, the n-3/n-6 values decreased in tanks, whereas an increasing trend was found in ponds, and values were significantly higher in ponds than in tanks (0.35 - 0.53 vs. 0.08 - 0.17). The results suggest raising Nile tilapia with azolladiets in ponds to improve growth and produce fish with a better FA composition, for the wellbeing of consumers.

关键词： azolla Oreochromis niloticus Concrete Tank Pond Growth Fatty Acid Phytoplankton

The Growth of Rice(Oryza sativa L.)on Non Tidal Lowland Soil through Enrichment of azolla and Different Level Fertilization Dosage(N and P)

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Journal of Environmental Science and Engineering(A) 2022年第3期11卷 95-100页

作者： Badriyah Nuni Gofar dan Dedik Budianta Department of Soil Science Faculty of AgricultureSriwijaya UniversityJl.Raya Palembang-Prabumulih KM 32 IndralayaOgan Ilir 30662Indonesia

Rice can grow in the non tidal lowland despite its limited soil *** of the biofertilizers that can be used to improve fertility is *** research aims to evaluate the growth of rice in non tidal lowlands using azolla to enrich the soil and different dosage of N(Nitrogen)and P(Phosphate)fertilization.A FCRD(Factorial Completely Randomized Design)was adopted consisting of 3 treatment factors,namely concentrations of N with doses of N1:46 kg/ha;N2:69 kg/ha;N3:92 kg/ha;P with P1:34 kg/ha;P2:68 kg/ha;and the application of azolla(A0:without azolla and A1:with 100 g/m2 azolla).The result showed that enriching the soil with azolla,fertilization dosage N and P,has a significant effect on plant height in the 4th WAP(Week After Planting).Furthermore,the interaction between azolla and N significantly influences panicle number,grain weight,and plant height at 2nd,4th,and 8th ***,the application of azolla,N,and P fertilization supplied nutrients to rice crops in the non tidal lowland.

关键词： azolla fertilization dosage P N non tidal lowland

Eocene Cooling Through Sustained Growth of azolla?

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地学前缘 2009年第S1期16卷 248-248页

作者： Eveline Speelman Henk Brinkhuis Monique van Kempen Jaap Sinninghe Damste Gert-Jan Reichart Faculty of Geosciences Utrecht University Utrecht University Radboud University Nijmegen Nederlands Instituut voor Onderzoek der Zee(NIOZ)

Through analyses of unique microlaminated sediments recovered from the Lomonosov Ridge during Integrated Ocean Drilling Program(IODP) Expedition 302,it became evident that enormous blooms of the free floating freshwater fern azolla occurred within the Arctic Basin during an extended period of～1.2 Ma in the *** timing of the azolla

关键词： azolla Eocene Arctic climate