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Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey

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Neural Regeneration Research 2021年第12期16卷 2446-2452页

作者： Xue-Yan Zhang Jun Li Cai-Juan Li Ying-Qi Lin Chun-Hui Huang Xiao Zheng Xi-Chen Song Zhu-Chi Tu Xiao-Jiang Li Sen Yan Guangdong Key Laboratory of Non-Human Primate Models Guangdong-Hongkong-Macao Institute of CNS RegenerationJinan UniversityGuangzhouGuangdong ProvinceChina Key Laboratory of CNS Regeneration Ministry of EducationJinan UniversityGuangzhouGuangdong ProvinceChina

In vitro cultures of primary cortical neurons are widely used to investigate neuronal ***,it has yet to be fully investigated whether there are significant differences in development and function between cultured rodent and primate cortical neurons,and whether these differences influence the utilization of cultured cortical neurons to model pathological *** in vitro culture techniques combined with immunofluorescence and electrophysiological methods,our study found that the development and maturation of primary cerebral cortical neurons from cynomolgus monkeys were slower than those from *** used a microelectrode array technique to compare the electrophysiological differences in cortical neurons,and found that primary cortical neurons from the mouse brain began to show electrical activity earlier than those from the cynomolgus *** cultured monkey cortical neurons developed slowly in vitro,they exhibited typical pathological features-revealed by immunofluorescent staining-when infected with adeno-associated viral vectors expressing mutant huntingtin(HTT),the Huntington’s disease protein.A quantitative analysis of the cultured monkey cortical neurons also confirmed that mutant HTT significantly reduced the length of ***,compared with the primary cortical neurons of mice,cultured monkey cortical neurons have longer developmental and survival times and greater sustained physiological activity,such as electrophysiological *** findings also suggest that primary cynomolgus monkey neurons cultured in vitro can simulate a cell model of human neurodegenerative disease,and may be useful for investigating time-dependent neuronal death as well as treatment via neuronal *** mouse experiments and protocols were approved by the Animal Care and Use Committee of Jinan University of China(IACUC Approval No.20200512-04)on May 12,*** monkey experiments were approved by the IACUC protocol(IACUC Approval N

关键词： axion-mea electrical activities human disease model Huntington’s disease HTT monkey neuron morphometric analysis mouse neuron neurodegenerative diseases primary culture

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