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avidin-biotin system pretargeting radioimmunoimaging and radioimmunotherapy and its application in mouse model of human colon carcinoma

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World Journal of Gastroenterology 2005年第40期11卷 6288-6294页

作者： Gui-Ping Li Hui Zhang Cheng-Mo Zhu, Jian Zhang Xu-Feng Jiang Departmnent of Nuclear Medicine NanfangHospital Southern Medical University Guangzhou 510515Guangdong Province China Department of Nuclear Medicine Ruijin Hospital Shanghai Second Medical University Shanghai 200025 China

AIM： To evaluate the multi-step pretargeting radioimmunoimaging （RII） and radioimmunotherapy （RTT） in nude mice bearing human colon carcinoma with avidin-biotin system labeled with 153^Sm. METHODS： Two- and three-step strategies for avidinbiotin system pretargeting techniques were established. In a three-step procedure, human colon carcinoma bearing nude mice were first injected with biotinylated monoclonal antibody （McAb-Bt） followed by cold avidin （Av） 48 h later and then 153^Sm-DB2 24 h thereafter; whereas the twostep procedure consisted of injection of 153^Sm-SA 48 h after pretargeting with biotinylated anti-CEA monoclonal antibody （CEA McAb-Bt）. SPECT imaging and biodistribution were performed at 4, 24, 48, or 72 h after injection of 153^Sm-labeled compounds. Five groups of nude mice subcutaneously grafted with human colon carcinoma were treated 3 d after grafting. One group received the injection with 100 μg CEA McAb-Bt followed by cold avidin （80 μg） after 2 d and 11.1 MBCl I53Sm-DB2 after 1 d. Four control groups were treated respectively with 11.1 MBq 153^Sm- CEA McAb, ii.i MBq 153^Sm-nmIgG, ii.i MBq 153^Sm-DB2, 100 μL normal saline. Toxicity was evaluated by changes of leukocyte count, and the efficacy by variation in tumor volume. Histological analyses of tumors were performed. RESULTS： The three-step procedure allowed faster blood clearance and yielded higher tumor blood ratios （5.76 at 4 h and 12.94 at 24 h） of the 153^Sm-DB2. The tumor was clearly visualized at 4 h in y-imaging after the injection of 153^Sm-DB2, while a significant accumulation of 153^Sm-SA in the tumor was observed only 24 h after the injection and tumor blood ratios at 4 and 24 h were 1.00 and 2.03, respectively, in the two-step procedure. Pretargeting RIT and 153^Sm-CEA McAb had a strong tumor-inhibiting *** tumor inhibitory rate was 80.67% and 78.44%, respectively, five weeks after therapy. Histopathological evidence also indicated radioactive damage in tumor tissues as necr

关键词： Radioimmunoimaging Radioimmunotherapy avidin-biotin Colon carcinoma

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The biodistribution and kinetics of the Samarium-153 labeled avidin,streptavidin and biotin

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Journal of Medical Colleges of PLA(China) 2001年第3期16卷 179-182页

作者：李贵平朱承谟江旭锋冯国伟张圣国 Department of Nuclear Medicine Nanfang Hospital First Military medical University Guangzhou 510515China Department of Nuclear Medicine Ruijin Hospital Shanghai Second Medical University Shanghai 200025China

Objective:To labelavidin(Av)or streptavidin(SA)with 153 Sm by takingadvantageof thehighbindingaffin-ityof biotinto Av or ***:A biotinderivative(DTPA-biotin)wasradiolabelledwith 153 Sm andthenboundto Av or *** vivo kineticsandbiodistributionof 153 Sm-labeledAv,SA ***:153 Sm-Avwascharacterizedby rapidclearancefromthebloodwithhighliverandrenaluptake;153 Sm-SAwas clearedfromthebloodslowlywithhighretentionintheliver,spleenandkidney,whereas 153 Sm-DTPA-biotinmetabolismwas accelerated,*** sion:Thebiodistributiondifferenceof SAandAvmay providean experimentalbasisfor theselectionof differentcomponentsof avidin-biotinsystemin pretagetingradioim-munoimagingandradioimmunotherapy.

关键词： avidin-biotin system 153Sm labeling biodistribution

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