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Evolutionary implications of avian infectious bronchitis virus(AIBV)analysis

Cell Research 2006年第3期16卷 323-327页

作者： Peng Shi~(1,2) Li Yu~2 Yun-xin Fu~3 Jing-Fei Huang~1 Ke-Qin Zhang~2 Ya-ping Zhang~(1,2)1 Laboratory of Cellular and Molecular Evolution,and Molecular Biology of Domestic Animals,Kunming lnstitute of Zoology,Chinese Academy of Sciences,Kunming 650223,China 2 Laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China 3 Human Genetics Center,University of Texas at Houston,Houston,Texas 77030,USA Laboratory of Cellular and Molecular Evolution and Molecular Biology of Domestic Animals Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China.

For developing efficient vaccines,it is essential to identify which amino acid changes are most important to the sur-vival of the *** investigate the amino acid substitution features in the avian infectious bronchitis virus (AIBV)antigenic domain of a vaccine serotype (DE072) and a virulent viral strain (GA98) to better understand adaptive evo-lution of *** addition,the SARS Coronavirus (SARS-CoV) was also analyzed in the same *** is interestingto find that extreme comparability exists between AIBV and SARS in amino acid substitution *** suggests thatamino acid changes that result in overall shift of residue charge and polarity should be paid special attention to duringthe development of vaccines.

关键词： avian infectious bronchitis virus SARS Coronavirus positive selection adaptive evolution vaccine development

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The Establishment of Fluorescent PCR Detection Method for avian infectious bronchitis virus

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Animal Husbandry and Feed Science 2012年第6期4卷 241-242,244页

作者： DU Xiong-wei LI Ye LI Zhen-rong Dalian Nationalities University Liaoning Entry-Exit Inspection and Quarantine Bureau

[ Objective] The aim was to to establish a kind of peculiar, sensitive and quick fluorescent PCR detection method. [Method] A peculiar, sensitive and quick method of fluorescent PCR detection for avian infectious bronchitis virus was established, the standard curve was built, specific primers, susceptibility and repeatability was detected. [ Result] This method diagnosed avian infectious bronchitis virus peculiarly, sensitively and quickly, simple and easy to use, time short, suitable for clinical testing. [ Conclusion] This research laid the foundation to diagnose avian infectious bronchitis virus.

关键词： avian infectious bronchitis virus Fluorescent PCR

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Isolation and Identification of avian infectious bronchitis virus

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Animal Husbandry and Feed Science 2012年第1期4卷 25-27页

作者： YU Di-he ZHANG Jian-jun WEI Bo Anhui Hewei Agricultural Development Co. Ltd. Guangde 242200 China Yangzhou VAC. BIO Engineering Co. Ltd. Yangzhou225000 China

[ Objective] The aim was to isolate and identify avian infectious bronchitis virus （IBV） from diseased chickens. [ Method] IBVs were iso- lated from the diseased chickens in a chicken farm in Anhui Province with blind passage method to observe virus pathogenicity. Then animal regres- sion test was made to replicate symptoms of bronchial congestion in SPF chickens and S1 gene segments were amplified and isolated, followed by comparison with IBV vaccine strains. [ Result] Detection of Hemagglutinating activity （HA） showed that allantoic fluid had no concerning effect on erythrocyte, suggesting that NDV and AIV were not included in the isolated viruses. However, the erythrocyte could be agglutinated with allantoic fluid treated with 1% of pancreatin, which is in consistent with biological characters of IBV. After SPF chickens were inoculated with the 6^th SPF al- lantoic fluid, bronchial congestion was replicated, proving that the isolated virus was avian IBV, named IBV XZ strain. [ Conclusion] This study pro- vides a theoretical basis for prevention of avian infectious bronchitis.

关键词： avian infectious bronchitis virus Bronchial congestion Isolation Identification

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Prokaryotic Expression of infectious bronchitis virus S1 Gene and Analysis of Biological Activity of Recombinant Protein

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Animal Husbandry and Feed Science 2010年第4期2卷 46-48页

作者： WANG Chun-li WANG Hong-jun ZHAO Quan College of Animal Science and Technology Jilin Agricultural University Changchun 130118 China Institute of Animal Husbandry and Veterinary Medicine Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 China

[Objective] To study the prokaryotic expression and antigenicity identification of S1 gene from avian infectious bronchitis virus （IBV）. [Method] The S1 gene was cloned into a pMD18-T vector to yield a recombinant plasmids pMD18-T-IBV-S1. Then S1 gene was inserted into the multiple cloning site of a prokaryotic expression vector pET-32a （＋）. The recombinant plasmid was transformed into E. coil BL21. The recombinant protein was induced by IPTG and measured by SDS-PAGE and western-blotting. [Result] The S1 gene was successfully expressed in E. coil BL21, the fusion proteins were about 66.0 kDa in a form of inclusion body. Western-blotting test showed that the recombinant proteins could be identified by IBV polyclonal antibody. [ Conclusion] The recombinant proteins of S1 gene have the antigenicity, which lays a good foundation for further research on new generation vaccine of IBV.

关键词： avian infectious bronchitis virus S1 gene Prokaryotic expression Western-blotting

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