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Genotoxicity of Three avermectins on Polypedates megacephalus Tadpoles Using the Comet Assay

Asian Herpetological Research 2016年第4期7卷 251-257页

作者： Baorong GENG Lingling ZHANG Yun JIANG Xiuping HUANG Jinmei DAI College of Life Sciences Fujian Normal University

avermectins are a new class of macrocyclic lactones derived from mycelia of the soil actinomycete, and are used as effective agricultural pesticides and antiparasitic agents. However, run-off from crops treated with avermectins may contaminate various bodies of water, and accumulated to certain concentrations to impact the development of aquatic animals. Here, we tested the genotoxicity of three avermectins （abamectin, ABM; ivermectin, IVM; and emamectin benzoate, EMB） on Polypedates megacephalus tadpoles by the alkaline single-cell gel electrophoresis assay. Tadpoles were treated for 48 h in the laboratory with different concentrations of these three agents, 0.006, 0.012, 0.018, 0.024, 0.030 mg/L for ABM, 0.003, 0.006, 0.009, 0.012, 0.015 mg/L for IVM and 0.04, 0.06, 0.08, 0.10, 0.12 mg/L for EMB, and then measured their DNA damage by the Comet assay tail factor %. The concentrations of resulted in highly significant increases in DNA damage of the tadpoles were found above the concentration threshold of 0.012 mg/ L ABM, 0.003 mg/L IVM and 0.06 mg/L EMB and linear correlations between the intensity of DNA damage and the concentrations of these three avermectins. Our results showed clearly that avermectins caused dose dependent DNA damage on amphibian tadpoles, and there might be a control on the misuse of avermectins.

关键词： Polypedates megacephalus tadpole avermectins abamectin ivermectin emamectin benzoate DNAdamage comet assay

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Adsorption of avermectins on activated carbon: Equilibrium, kinetics, and UV-shielding

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中国有色金属学会会刊：英文版 2009年第S3期19卷 845-850页

作者：顾微孙长娇刘琪崔海信 Institute of Environment and Sustainable Developmentin Agriculture Chinese Academy of Agricultural Sciences

The adsorption of biopesticide avermectins onto activated carbon from ethanol solution with different initial concentrations at 303.15 K was performed. The obtained equilibrium and kinetic data of the adsorption process were assayed to evaluate the adsorption potential of activated carbon for avermectins. The results show that the activated carbon is effective for the adsorption of avermectins. Moreover, the adsorption of avermectins onto activated carbon agrees with Langmuir isotherm model, while pseudo-second-order kinetics model is better fitable for such adsorption process. In addition, activated carbon can efficiently protect adsorbed avermectins from photodegradation.

关键词： activated carbon avermectins adsorption UV-shielding

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Interrogation of Streptomyces avermitilis for efficient production of avermectins

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Synthetic and Systems Biotechnology 2016年第1期1卷 7-16页

作者： Jinsong Chen Mei Liu Xueting Liu Jin Miao Chengzhang Fu Heyong Gao Rolf Muller Qing Zhang Lixin Zhang Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina Graduate University of Chinese Academy of Sciences BeijingChina Helmholtz Institute for Pharmaceutical Research Saarland Helmholtz Centre for Infection Research66123SaarbruckenGermany Department of Pharmaceutical Biotechnology Saarland UniversityBuilding C 2.366123SaarbruckenGermany Inner Mongolia New Veyong Biochemical Co. LtdInner Mongolia Dalad BannerChina

The 2015 Nobel Prize in Physiology or Medicine has been awarded to avermectins and artemisinin,*** produced by Streptomyces avermitilis are excellent anthelmintic and potential antibiotic *** wild-type strains only produce low levels of avermectins,much research effort has focused on improvements in avermectin production to meet the ever increasing demand for such *** review describes the strategies that have been widely employed and the future prospects of synthetic biology applications in avermectin yield *** the help of genome sequencing of *** and an understanding of the avermectin biosynthetic/regulatory pathways,synthetic and systems biotechnology approaches have been applied for precision *** focus on the design and synthesis of biological chassis,parts,devices,and modules from diverse microbes to reconstruct and optimize their dynamic processes,as well as predict favorable effective overproduction of avermectins by a 4Ms strategy(Mine,Model,Manipulation,and Measurement).

关键词： avermectins Metabolic engineering Streptomyces avermitilis Synthetic biology

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avermectins发酵培养基的研究

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中国抗生素杂志 2001年第3期26卷 176-180,193页

作者：宋渊李季伦王得明刘世宽中国农业大学生物学院北京100094 齐鲁制药厂济南250400

以 Streptomycesavermitilis ATCC312 72和它的突变株为试验菌株进行 avermectins发酵培养基的研究。最初筛选到的发酵培养基中必需加入番茄酱和鲜酵母 ,进一步通过对 170多种发酵培养基的筛选实验 ,选择出一种以淀粉、酵母粉、豆饼粉... 详细信息

以 Streptomycesavermitilis ATCC312 72和它的突变株为试验菌株进行 avermectins发酵培养基的研究。最初筛选到的发酵培养基中必需加入番茄酱和鲜酵母 ,进一步通过对 170多种发酵培养基的筛选实验 ,选择出一种以淀粉、酵母粉、豆饼粉和无机盐为营养物的培养基 ,并以它为基础研究了碳源、氮源、无机盐等对 avermectins生物合成的影响。经多次培养基的优化试验 ,突变株 Sa- 76 - 9摇瓶发酵效价达 35 0 0～40 0

关键词： SteptomycesAvermitilis avermectins 培养基发酵大环内酯类

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avermectins菌丝提取液的分析与主要成分的分离富集

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中国抗生素杂志 2008年第5期33卷 280-286页

作者：刘吉常志东杨明德党杰何艺清华大学核能与新能源技术研究院北京100084 中国科学院过程工程研究所北京100081

采用高效液相色谱(HPLC)-紫外检测(UVD)/蒸发光散射检测(ELSD)的方法分析avermectins菌丝提取液,发现主要成分为具有紫外吸收和无紫外吸收的物质。利用硅胶吸附柱层析方法分离富集了其中的主要成分。对几种有紫外吸收的组分(Ala、A2a、... 详细信息

采用高效液相色谱(HPLC)-紫外检测(UVD)/蒸发光散射检测(ELSD)的方法分析avermectins菌丝提取液,发现主要成分为具有紫外吸收和无紫外吸收的物质。利用硅胶吸附柱层析方法分离富集了其中的主要成分。对几种有紫外吸收的组分(Ala、A2a、Bla、B2a)进行了核磁共振(NMR)分析,根据异核单量子相干谱(HSQC)对所有的^(13)C-NMR和~1H-NMR谱带进行了归属。鉴定了无紫外吸收的物质中含有糖类物质。