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In VitroCharacterization of Cell Surface Properties of 14 Vaginal LactobacillusStrains as Potential Probiotics

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Advances in Microbiology 2021年第2期11卷 144-155页

作者： Shao-Ji Li Jae-Seong So SignalDT Biotechnologies (SZ) Inc. Shenzhen China Department of Biological Engineering Inha University Incheon Korea

Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Human-origin Lactobacillus is a preferable source of probiotic bacteria. This study screened 14 vaginal Lactobacillus strains as probiotic candidates by investigating probiotic-related cell surface characteristics including cell surface hydrophobicity (CSH), Lewis acidity/basicity, autoaggregation, and biofilm formation. Moderate to high CSH and autoaggregation, high basicity and low acidity were prevalent in the 14 tested strains. Biofilm formation varied in a large range among the 14 tested strains. CSH showed a high correlation with Lewis acidity and autoaggregation, while Lewis acidity was highly correlated with autoaggregation and biofilm formation. Four strains were selected as promising probiotic strains. This study was the first one to compare antibiotic sensitivity between biofilm-forming cells and planktonic cells of Lactobacillus species, and found that biofilm-forming cells of a L. fermentum strain had a significantly higher survival rate than planktonic cells in cefotaxime, cefmetazole and tetracycline, but were as sensitive to oxacillin and ampicillin as planktonic cells were.

关键词： Lactobacillus Probiotics Cell Surface Hydrophobicity Lewis Acidity Lewis Basicity autoaggregation Biofilm Antibiotics

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A Novel Cellular Autoaggregative Developmentally CRP Regulated Behaviour Generates Massively Chondrule-Like Formations over Surface of Old Escherichia coliK-12 Macrocolony Biofilms

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Advances in Bioscience and Biotechnology 2014年第9期5卷 727-739页

作者： José María Gómez Gómez Ricardo Amils Centro de Astrobiología (CSIC-INTA) (Associated with NASA Astrobiology Institute) Ctra Ajalvir km 4 28850 Torrejón de Ardoz Madrid Madrid Spain Centro de Biología Molecular Severo Ochoa C/Nicolás Cabrera No 1 Departamento de Virología y Microbiología Laboratorio 104 Campus of Universidad Autónoma de Madrid Madrid Spain

How Escherichia coli bacteria develop a particular colonial, 3-D biofilm morphological pattern is still a poorly understood process. Recently, we reported a new E. coli K-12 morphotype exhibited by old macrocolonies described as volcano-like. The formative developmental process of this morphotype has been presented as a suitable experimental model for the study of 3D patterning in macrocolony biofilms. Here, we report the optical microscopy observations and genetic analysis that have unveiled the existence of a novel autoaggregative behaviour which generates massive lumpiness over the surface of the volcano-like macrocolonies. These lumpy formations are generated by the autoaggregation and strong interaction of tightly packed bacterial cells in structures with a chondrule-like appearance which give the colony’s surface its characteristic microscopic lumpy phenotype. Furthermore, they exhibit different levels of maturation from the edge to the center of the colony. Hence, its generation appears to follow a spatiotemporal program of development during the macrocolony’s morphogenesis. Interestingly, the agar’s hardness influences the morphology exhibited by these formations, with high agar concentration (1.5%, 15 g/L) suppressing its development. This new auto-aggregative E. coli’s behaviour does not require the activity of the biofilm master regulator CsgD, the adhesiveness of flagella, pili type 1, adhesin Ag43, β-1,6-N-acetyl-D-glucosamine polymer-PGA, cellulose or colanic acid, but it is under glucose repression and the control of cAMP receptor protein (CRP). The possible physiological role of these chondrule-like formations in the adaptability of the colony to different stressful environmental conditions is discussed.

关键词： Biofilms E. coli Volcano-like morphotype Macrocolony autoaggregation Chondrule-Like Formations CRP Regulated Behaviour Ag43 Independent autoaggregation β-1 6-N-Acetyl-D-Glucosamine Polymer (PGA) Independent autoaggregation

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