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Nerve growth factor expression in astrocytoma and cerebrospinal fluid： a new biomarker for prognosis of astrocytoma

Chinese Medical Journal 2011年第14期124卷 2222-2227页

作者： LI Qiao-yu YANG Yong ZHANG Yan ZHANG Zhi-jian GONG Ai-hua YUAN Zhi-cheng LU Pei-son ZHAN Li-ping WANG Peng FENG Yun XU Wen-lin .Department of Neurosurgery the Affiliated People's Hospital of Jiangsu University ZhenjiangJiangsu 212002 China Institute of Clinical Laboratory Medicine Jiangsu UniversityZhenjiang Jiangsu 212013 China Central Laboratory the Affiliated People's Hospital of Jiangsu University ZhenjiangJiangsu 212002 China

Background Recent studies have discovered that nuclear translocation of nerve growth factor （NGF） and its receptor fragments function differently from the traditional model. This study aimed to uncover the nuclear expression of NGF in astrocytoma and its biological *** Ninety-four paraffin-embedded astrocytoma specimens were subjected to immunohistochemical （IHC） and hemotoxylin ＆ eosin （HE） staining. Preoperative cerebrospinal fluid （CSF） specimens and intraoperative snap-frozen astrocytoma tissues were assayed for NGF expression by ELISA and Western blotting. The outcome of patients who contributed samples was tracked. Each ten tissue samples from patients with traumatic brain injury who had received decompression surgery and CSF samples from patients undergoing spinal anesthesia but with no history of nervous system disease were taken as *** NGF-positive immunoreactive products were distributed in both the cytoplasm and nucleus of astrocytoma, but were only located in the cytoplasm of traumatic brain injury （TBI） tissue. NGF nuclear-positive rate （NPR） of grades Ⅲ-Ⅳ astrocytomas （70.0%） was higher than that of grades Ⅰ-Ⅱ astrocytoma （28.6%, P＜0.05）. NGF-NP expression positively correlated with the NGF concentration in cerebrospinal fluid （CSF）（r=0.755, P＜0.01）. Kaplan-Meier survival analysis indicated that the median survival time was 25 months for NGF-NP astrocytoma grade Ⅰ-Ⅱ patients and 42 months in NGF nuclear negative （NGF-NN） astrocytoma grade Ⅰ-Ⅱ patients （P＜0.05）. In astrocytoma Ⅲ-Ⅳ patients, the median survival was 7 months for NGF-NP patients and 24 months for NGF-NN patients （P＜0.01）. Two types of NGF with molecular weights of 13 and 36 kDa were present in astrocytoma, but only the 36 kDa NGF was found in the CSF. NGF expression elevated as the malignancy *** NGF-NP expression and NGF level in CSF were significant prognostic factors in astrocytoma *** of the easy access of CSF, it may be deve

关键词： nerve growth factor astrocytoma cell nucleus prognosis

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Identification of tumor invasion-related differentially expressed genes in different grades and all-trans retinoic acid-treated astrocytoma cell lines

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Neural Regeneration Research 2008年第11期3卷 1222-1228页

作者： Yi Zeng Zhong Yang Yangyun Han Chao You Department of Neurosurgery West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Department of Neurosurgery Deyang People＇s Hospital Deyang 618000 Sichuan Province China Department of Neurobiology Third Military Medical University of Chinese PLA Chongqing 400038 China

BACKGROUND： Although several genetic aberrations and gene expressional changes have been shown to exist in tumors and different grades of astrocytomas, as well as in normal tissues, the gene profiling and genetic pathways associated with malignant transformation and progression remain unclear. OBJECTIVE： To identify differentially expressed genes related to tumor invasion from various grades and all-trans retinoic acid （ATRA）-treated astrocytoma cell lines by cDNA microarray. DESIGN, TIME AND SETTING： In vitro gene experiment was performed at the Department of Neurobiology, Third Military Medical University of Chinese PLA from January to October 2007. MATERIALS： Two different grades of astrocytoma cell lines CHG-5 （WHO grade II ） and SHG-44 （WHO grade IV） were developed by our laboratory; a cell differentiation-inducing agent ATRA and a human cDNA microarray technology were used to determine differentially expressed genes （City University of Hong Kong）. METHODS： Total RNA was extracted using the Trizol test kit. Reverse transcription was performed using Superscript 11 reverse transcriptase. The cDNA product （target DNA） was marked with fluorochromes Cy3 （normal SHG-44） and Cy5 （CHG-5 or ATRA-treated SHG-44）, followed by chip hybridization. MAIN OUTCOME MEASURES： Gene expression profiles of CHG-5 vs. SHG-44 and ATRA-treated vs. normal SHG-44 were performed to identify differentially expressed genes. Several of these genes were randomly selected for Northern Blot analysis. The identification of genes that were similarly regulated （overlapping） was performed by comparing gene expression profiles between CHG-5 and SHG-44 cells, and between SHG-44 cells with or without treatment with ATRA. RESULTS： No significant differences were observed between CHG5 and SHG-44 cell line morphology. Under confocal microscopy, GFAP staining intensity of CHG5 cells was greater than SHG-44 cells （t = 6.078 P = 0.004）. Growth curve analysis demonstrated that the speed of SHG-44 cell growth

关键词： all-trans retinoic acid astrocytoma cDNA microarray CHG-5 invasion SHG-44

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Prevention against diffuse spinal cord astrocytoma: can the Notch pathway be a novel treatment target?

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Neural Regeneration Research 2015年第2期10卷 244-251页

作者： Jian-jun Sun Zhen-yu Wang Ling-song Li Hai-yan Yu Yong-sheng Xu Hai-bo Wu Yi Luo Bin Liu Mei Zheng Jin-long Mao Xiao-hui Lou Department of Neurosurgery Peking University Third Hospital Peking University China Stem Cell Research Center Peking University Health Science Center Clinical Stem Cell Center Peking University Third Hospital Clinical Laboratory of Tissue & Cell Research Center Department of Biotech Treatment Logistics College of Chinese People’s Armed Police Force Department of Neuroradiology Peking University Third Hospital Department of Neurology Peking University Third Hospital Neurosurgical Department Peking Union Medical College Hospital Department of Neurosurgery Rui’an People’s Hospital Wenzhou Medical University

This study was designed to investigate whether the Notch pathway is involved in the develop-ment of diffuse spinal cord astrocytomas. BALB/c nude mice received injections of CD133＋and CD133？ cell suspensions prepared using human recurrent diffuse spinal cord astrocytoma tissue through administration into the right parietal lobe. After 7–11 weeks, magnetic resonance imaging was performed weekly. Xenografts were observed on the surfaces of the brains of mice receiving the CD133＋ cell suspension, and Notch-immunopositive expression was observed in the xenografts. By contrast, no xenografts appeared in the identical position on the surfaces of the brains of mice receiving the CD133？ cell suspension, and Notch-immunopositive expres-sion was hardly detected either. Hematoxylin-eosin staining and immunohistochemical staining revealed xenografts on the convex surfaces of the brains of mice that underwent CD133＋ astro-cytoma transplantation. Some sporadic astroglioma cells showed pseudopodium-like structures, which extended into the cerebral white matter. However,it should be emphasized that the sub-cortex xenograft with Notch-immunopositive expression was found in the fourth mouse received injection of CD133？ astrocytoma cells. However, these ifndings suggest that the Notch pathway plays an important role in the formation of astrocytomas, and can be considered a novel treat-ment target for diffuse spinal cord astrocytoma.

关键词： nerve regeneration astrocytoma mice immunodeficiency (BALB/c) mice Notch nestin glial fibrillary acidic protein CD133 spinal cord brain MRI neural regeneration

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astrocytoma simultaneously present with Meningioma-a report of two cases and review of the literature

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Chinese Neurosurgical Journal 2016年第2期2卷 -页

作者： Aihemaiti Hasimu Qiang Fu Qingjiu Zhou Shaoshan Li Xiaofeng Zhu Chen Liu Dangmuren Jiafu Geng Bo Liu Department of Neurosurgery First Af iliated Hospital of Xinjiang Medical University 137 Liyushan Road Xinshi District Urumqi Xinjiang 830054 PR of China

This research paper will discuss the surgical management and possible mechanisms, as well as enlighten other features which can explain meningioma and astrocytoma simultaneously occurrences including statistical coincidence, the role of common carcinogens, autocrine growth factors and their pathogenic correlations. We describe two patients with simultaneous presentation of meningioma with astrocytoma. A correct preoperative radiological diagnosis was made in both patients and their tumors were completely removed microscopically. Pathological examinations confirmed that one among them was meningioma and the other an anaplastic astrocytoma. We reviewed the studies carried out regarding meningioma associated with astrocytoma in China as well as in other countries. In the absence of phacomatosis or prior radiation therapy, the reason for the simultaneous occurrence of astrocytoma with meningioma is not clear, and these tumors could be merely a statistical coincidence. Careful preoperative radiological evaluation and surgical management is of great importance in these patients.

关键词： astrocytoma Meningioma Simultaneous tumors Surgical management

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Screening of differentially expressed genes related to differentiation and proliferation by gene expression profiling of different grade astrocytoma cell lines

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Neural Regeneration Research 2008年第3期3卷 245-249页

作者： Yi Zeng Zhong Yang Yangyun Han Chao You Department of Neurosurgery West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Department of Neurosurgery People＇s Hospital of Deyang City Deyang 618000 Sichuan Province China Department of Neurobiology the Third Military Medical University Chongqing 400038 China

BACKGROUND： The detection of differential gene expression in brain is possible by cDNA microarray technology, and the screening of differentially expressed genes might provide a biological basis for gene-targeted therapy for tumors. OBJECTIVE： To detect the differential expression of genes among astrocytoma SHG-44 （WHO grade Ⅳ）, CHG-5 （WHO grade Ⅱ）, and ATRA-treated SHG-44 cell lines by cDNA microarray. DESIGN： Laboratory experiments in vitro. SETTING： Department of Neurobiology, the Third Military Medical University. MATERIALS： The experiment was performed at the Department of Neurobiology in the Third Military Medical University of the Chinese PLA from January to October 2007. The SHG-44 cell line （WHO grade Ⅳ） was established by Prof. Ziwei Du, and the CHG-5 cell line （WHO grade Ⅱ） was set up by Prof. Xiuwu Bian from the Third Military Medical University of the Chinese PLA. The cDNA microarray containing 9182 known genes was prepared and provided by Dr. Yang Zhong at the City University of Hong Kong. METHODS： To screen differentially expressed genes from the gene expression profiles detected by cDNA microarray comparisons were made between CHG-5 and SHG-44 cells and between SHG-44 cells with or without treatment with 10 μmol/L ATRA. Some differentially expressed genes were selected randomly for Northern Blot analysis to confirm the results of the microarray. The determination criteria for differential gene expression were as follows. ① The ratio of Cy5 signal to Cy3 was greater than 2.0 or less than 0.5. ② The results of the triplicate microarray hybridizations showed the same trend in three experiments. ③ A gene appeared at least two times on the triplicate microarray hybridizations, and the 3^rd value did not show a contradictory trend. A normalized ratio of Cy5 intensity to Cy3 greater than 2.0 or less than 0.5 was considered to represent up-regulated or down-regulated gene expression, respectively. MAIN OUTCOME MEASURES： The identification of genes that were

关键词： differentiation proliferation CHG-5 SHG-44 astrocytoma cDNA microarray

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Misdiagnosed Angioimmunoblastic T-cell Lymphoma Secondary to Cranial astrocytoma

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Chinese Journal of Cancer Research 2009年第4期21卷 340-343页

作者： Jia Wei Xian-sheng Liu Yong-jian Xu Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Respiratory Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

A case of angioimmunoblastic T-cell lymphoma （AITL） which was misdiagnosed as adult Still＇s disease was presented. The clinical and laboratory characteristics of this case and related literatures were analyzed and reviewed. The patient was finally diagnosed as AITL （Ann Arbor classification： Stage IIIB） secondary to cranial astrocytoma （WHO classification： Stage III）, complicated with severe pulmonary infection because of long time treatment of corticosteroid and misdiagnosis （about one and a half year）. It is concluded that AITL is a rare disease which was easily misdiagnosed. The diagnosis of AITL should combine the clinical manifestation with pathological biopsy as well as corresponding immunohistochemical tests.

关键词： AITL astrocytoma Immunohistochemistry Diagnosis

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Volumetric Assessment of Corticospinal Tract Infiltration by astrocytoma Using Diffusion Tensor Tractography

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Open Journal of Medical Imaging 2014年第2期4卷 65-71页

作者： Marwa Adel Shaaban Taha Kamel Alloush Mohamed Hamdy Ibrahim Ahmed Abd el bar Sherif Hashim Morad Department of Neurology Ain Shams University Cairo Egypt Department of Neurosurgery Ain Shams University Cairo Egypt Department of Radiology Misr University for Science and Technology Cairo Egypt

Objectives: The aim of the study is to assess volumetric Corticospinal Tract infiltration by Astro-cytoma using Diffusion Tensor Tractography. Material and Methods: Preoperative, anatomic (T1- and T2-weighted) and diffusion tensor MRI were performed in 9 patients with supratentorial gliomas (WHO grade II and III). The tumors were manually segmented from the T1- and T2-weighted MRI, and their volume calculated. A three-dimensional tractography was performed in each case. A second segmentation and volume measurement was performed on the tumor regions intersecting adjacent white matter fiber tracts. Results: We identified that white matter tracts were infiltrated by 6 astrocytoma tumors. The median tumor volume (±standard deviation) in our patient population was 33 ± 26.82 ml. The median tumor volume (±standard deviation) infiltrating white matter fiber tracts was 4.15 ± 9.23 ml. The median fraction of tumor volume infiltrating white matter fiber tracts was 26.3% ± 10.1%. Conclusions: Diffusion tensor MR Tractography is a reliable preoperative assessment tool since it detects extensive white matter infiltration by astrocytoma irrespective of brain tumors volume. Recommendations: Prospective large population studies are required to clarify how infiltration relates to tumor location.

关键词： Tractography astrocytoma Brain Tumor Corticospinal Tract Infiltration

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Solitary Retinal astrocytoma: A Case Series

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Open Journal of Pathology 2013年第2期3卷 60-64页

作者： Takafumi Fuchino Tomayoshi Hayashi Kiyoshi Suzuma Asuka Taneoka Kuniko Abe Naoe Kinoshita Haruna Yasui Takashi Kitaoka Junya Fukuoka Department of Pathology Nagasaki University Hospital Nagasaki Japan

Background: Astrocytic tumors of the retina are rare. We report and discuss the clinical features of two cases of retinal astrocytoma along with presenting a current literature review. Case Presentation: Case 1 was a 46-year-old Japanese female who became aware of her decreased visual acuity. A fundus photograph indicated the presence of a 5 mm hemispherical yellow-white tumor, retinal edema and hard exudate around the tumor. In case 2, a 36-year-old Japanese female became aware of her myodesopsia, and presented with a retinal tumor and vitreous hemorrhage in her right eye. Since the tumors occurred in the peripheral retina in both cases, endoresections were performed. Histological examination showed that the tumors were composed of spindle-shaped cells with small nuclei, which was consistent with astrocytes. Conclusion: Pathologically, it can be difficult to differentiate astrocytic tumors. Therefore, when making a diagnosis, it is important that comprehensive examinations be done in conjunction with the clinical findings. Since retinal astrocytoma has a favorable prognosis, provided proper treatment is administered, utilization of endoresection for peripheral astrocytoma may be advantageous in this patient group.

关键词： Retina astrocytoma Pathology

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Expression Difference of neural cell adhesion molecule in brain astrocytoma

Expression Difference of neural cell adhesion molecule in br...

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2011中华医学会神经外科学学术会议

作者： yanjin wang1 jiasheng fang1 fenghua chen1 lei huo1 mingyu zhang1 jun wu1 1.Department of Neurosurgery Xiangya Hospital Central South University, Changsha 4 1 0008

结论g>Objective Objective To found out NCAM expression difference in astrocytoma at mRNA and protein level. Methods Total RNA were were extracted in 43 astrocytoma and 1 U251, after purifiy through three pair primers(... 详细信息

关键词： neural cell adhesion molecule astrocytoma

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CCNG2 overexpression mediated by AKT inhibits tumor cell proliferation in human astrocytoma cells

CCNG2 overexpression mediated by AKT inhibits tumor cell pro...

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第十三届中国医师协会神经外科医师年会

作者： Danfeng Zhang Chunhui Wang Zhenxing Li Yiming Li Dawei Dai Kaiwei Han Liquan Lv Yicheng Lu Lijun Hou Junyu Wang Department of neurosurgery Changzheng HospitalSecond Military Medical University

The cyclin family protein CCNG2 has an important inhibitory role in cancer initiation and progression but the exact mechanism is still *** the present study,we examined the relationship between CCNG2 and the malignancy of astrocytomas and whether the AKT pathway,which is upregulated in astrocytomas,may inhibit CCNG2 ***2 expression was found to be negatively associated with the pathological grade and proliferative activity of astrocytomas,as the highest expression was found in control brain tissue（N=31）whereas the lowest expression was in highgrade glioma tissue（N=31）.Additionally,CCNG2 overexpression in glioma cell lines T98 G and U251 inhibited proliferation and arrested cells in the G0/G1 ***,CCNG2 overexpression could increase glioma cells *** contrast,AKT activity increased in glioma cells that had low CCNG2 *** of CCNG2 was higher in cells treated with the AKT kinase inhibitor MK-2206 indicating that the presence of phosphorylated AKT may inhibit the expression of *** of AKT also led to decreased colony formation in T98 G and U251 cells and knocked down of CCNG2 reversed the ***,overexpression of CCNG2 in glioma cells reduced tumor volume in a murine *** conclude,low expression of CCNG2 correlated with the severity astrocytoma and CCNG2 overexpression could induce apoptosis and inhibit *** of AKT activity increased the expression of *** present study highlights the regulatory consequences of CCNG2 expression and AKT activity in astrocytoma tumorigenesis and the potential use of CCNG2 in anticancer treatment.

关键词： astrocytoma AKT pathway Carcinogenesis CCNG2 MK 2206

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