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第十五届中国科协年会第21分会场：中药与天然药物现代研究学术研讨会

作者： HAO Baocheng YANG Xianpeng WANG Xuehong LIU Jianzhi WANG Baohai LIU Yu GUO Wenzhu GUO Zhiting SHANG Ruofeng HU Yonghao LIANG Jianping Institute of Husbandry and Pharmaceutical Sciences of CAAS

Locoweed is a poisonous plant, which wildly distributed in most area of the world, it can cause livestock poisoning or death with significant economic loss. The astragalus strictus belongs to a species of locoweed .It is mainly distributed in Tibet of China and is a serious hazard to the local livestock industry. The objective of this study is extracting and purifying condition of Swainsonine from astragalus strictus with the microwave-assisted extraction method. An optimum extracting technology of SW from astragalus strictus was investigated through the orthogonal experiment under the microwave assistance conditions, then the content of swainsonine was analyzed with gas chromatography(GC)method. The optimized extraction conditions were as follow: at room temperature, the time of using the petroleum ether ultrasonic to make the Swainsonine degreased with ultrasonic was 1h, and then use the method of extraction with microwave-assisted, solid-liquid ratio (M: V) 1:10, microwave frequencies 280W , extraction time 20 s. Under the above conditions, the extraction percentage of the swainsonine was 0.009264%.Extracting swainsonine with microwave-assisted extraction method has characteristics of rapid, efficient, convenient and low energy consumption. It is benefit for producing swainsonine from astragalus strictus.

关键词： extraction astragalus strictus swainsonine microwave gas chromatography (GC)

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Cellulase Extraction and Gas Chromatography Detection Technology of Swainsonine from astragalus strictusin in Tibet

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Agricultural Science & Technology 2013年第8期14卷 1163-1166页

作者：郝宝成杨贤鹏王学红胡永浩梁剑平甘肃省新兽药工程重点实验室/农业部兽药药剂开发重点实验室/中国农业科学院兰州畜牧与兽药研究所甘肃兰州730050 甘肃农业大学兽医学院甘肃兰州730070

Locoweed is a poisonous plant wildly distributed in most areas of the world,which causes livestock poisoning or death with serious economic *** astragalus strictus belongs to a species of *** is mainly distributed in Tibet of China and is a serious hazard to the local livestock *** objective of this study is extracting and purifying condition of Swainsonine from astragalus strictus with the cellulase extraction *** optimum extracting technology of SW from astragalus strictus was investigated through the orthogonal experiment under the cellulose assistance conditions,and then the content of swainsonine was analyzed with gas chromatography （GC） *** optimized extraction conditions were as follow：The crushed mesh is 40; solid-liquid ratio is 1∶40 （g/ml）; enzyme dosage is 3.5％; enzymatic time is 3 *** the above conditions,the extraction percentage of the swainsonine was 0.003 941％.The conditions for extracting swainsonine with cellulase extraction method are mild,it is easy to utilize industrial *** is benefit for producing swainsonine from astragalus strictus.

关键词： Extraction astragalus strictus Swainsonine Cellulase GC

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