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Effect of cultivating conditions on α-galactosidase production by a novel aspergillus foetidus zu-g1 strain in solid-state fermentation

Effect of cultivating conditions on α-galactosidase product...

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： LIU Cai-qin CHEN Qi-he CHENg Qian-jun WANg Jin-ling HE guo-qing~(+++) (Department of Food Science and Nutrition,Zhejiang University,Hangzhou 310029,China)

The work is intended to achieve optimum culture conditions of α-galactosidase production by a mutant strain aspergillus foetidus zu-gl in solid-state fermentation(SSF). Certain fermentation parameters involving moisture content,incubation temperature,cultivation period of seed,inoculum volume,initial pH value,layers of pledget,load size of medium and period of cultivation were investigated *** optimal cultivating conditions of α-galactosidase production in SSF were 60% initial moisture of medium,28℃ incubation temperature,18 h cultivation period of seed,10% inoculum volume,5.0～6.0 initial pH of medium, 6 layers of pledget and 10g dry matter *** the optimized cultivation conditions,the maximum α-galactosidase production was 2037.51 U/g dry matter near the 144th hour of fermentation.

关键词： α-galactosidase Culture condition aspergillus foetidus zu-g1 Solid-state fermentation

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Optimization of the Fermentation Medium for Alpha-galactosidase Production from Aspergiilus foetidus zu-g1 Using Response Surface Methodology

Optimization of the Fermentation Medium for Alpha-Galactosid...

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： Caiquin LIU Hui RUAN Huafeng SHEN Qihe CHEN Bing ZHOU Yi LI AND guoqing HE (Authors Liu,Ruan,Shen,Chen,Zhou,and He are with Dept.of Food Science Nutrition,Zhejiang Univ.,Hangzhou,310029,China.Author Li is with Dept.of PlantSciences,NorthDakota StateUniv.,Fargo,ND58105,U.S.A.)

The optimization of fermentation medium for alpha-galactosidase production by aspergillus foetidus zu-gl was investigated in shaker flask fermentation.A one-factor-at-a-time experiment was used to screen the preferable nutriment(carbon sources, nitrogensources,andessential elements)for alpha-galactosidase production.A fractional factorial design was used to screen the main 5 factors,soybean meal,wheat bran,KH2PO4,FeSO4·7H2O, and the medium initial pH,that affected the production of *** central composite experimental design was further adopted to derive a statistical model for optimizing the composition of the fermentation *** experimental results showed that the optimum fermentation medium for alpha-galactosidase production by aspergillus foetidus zu-gl was composed of 3.2% soybean meal(w/v),2% wheat bran(w/v),0.1% KH2PO4(w/v),and 0.05% FeSO4.7H20(w/v);initial medium pH was *** results further predicted that alpha-galactosidase activity reached 64.75 U/mL after 95-h incubation in this medium,which was approximately 7 times higher than that incubated in the nonoptimized *** time course of alpha-galactosidase production in the optimized medium composition was also carried out to validate the model.

关键词： alpha-galactosidase aspergillus foetidus zu-g1 crude sources medium optimization response surface Methodology

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Effect of cultivating conditions on α-galactosidase production by a novel aspergillusfoetidus zu-g1 strain in solid-state fermentation

Effect of cultivating conditions on α-galactosidase product...

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饲用酶制剂开发与应用技术交流研讨会

作者： LIU Cai-qin,CHEN Qi-he,CHENg Qian-jun,WANg Jin-ling,HE guo-qing~(?) (Department of Food Science and Nutrition.Zhejiang University,Hangzhou 310029,China)

The work is intended to achieve optimum culture conditions ofα-galactosidase production by a mutant strain aspergillus foetidus zu-g1 in solid-state fermentation(SSF).Certain fermentation parameters involving moisture content,incubation temperature,cultivation period of seed,inoculum volume,initial pH value,layers of pledget,load size of medium and period of cultivation were investigated *** optimal cultivating conditions ofα-galactosidase production in SSF were 60% initial moisture of medium.28℃incubation temperature.18 h cultivation period of seed.10%inoculum volume.5.0～6.0 initial pH of medium.6 layers of pledget and 10 g dry matter *** the optimized cultivation conditions,the maximumα-galactosidase production was 2037.51 U/g dry matter near the 144th hour of fermentation.

关键词： α-galactosidase Culture condition aspergillus foetidus zu-g1 Solid-state fermentation

来源： cnki会议评论

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