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Response surface methodology for optimizing the fermentation medium of alpha-galactosidase in solid-state fermentation

Response surface methodology for optimizing the fermentation...

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： C.Q.Liu Q.H.Chen B.Tang H.Ruan G.Q.He (Department of Food Science and Nutrition,Zhejiang University,Hangzhou 310029,China)

Aims:Alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides(RO)that are the factors primarily responsible for flatulence upon ingestion of soybean-derived *** objective of the current work was to develop an optimal culture medium for the production of alpha-galactosidase in solid-state fermentation(SSF) by a mutant strain aspergillus foetidus.,Methods and Results:Response surface methodology (RSM)was applied to evaluate the effects of variables,namely the concentrations of wheat bran, soybean meal,KHPO,MnSO·HO and CuSO·5HO on alpha-galactosidase production in the solid substrate.A fractional factorial design(FFD)was firstly used to isolate the main factors that affected the production of alpha-galactosidase and the central composite experimental design (CCD)was then adopted to derive a statistical model for optimizing the composition of the fermentation *** experimental results showed that the optimum fermentation medium for alpha-galactosidase production by aspergillus foetidus ZU-Gl was composed of 8.2137 g wheat bran,1.7843 g soybean meal,0.001 g MnSO·HO and 0.001 g CuSO·5HO in 10g dry matter fermentation medium.,Conclusions:After incubating 96 h in the optimum fermentation medium,alpha-galactosidase activity was predicted to be 2210·76 U gdry matter in 250 ml shake *** the present study,alpha-galactosidase activity reached 2207·19 U gdry matter. Significance and Impact of the Study:Optimization of the solid substrate was a very important measure to increase enzyme activity and realize industrial production of *** process of alpha-galactosidase production in laboratory scale may have the potential to scale-up.

关键词： alpha-galactosidase aspergillus foetidus medium optimization response surface methodology solid-state fermentation.

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Response surface methodology for optimizing the fermentation medium of alpha-galactosidase in solid-state fermentation

Response surface methodology for optimizing the fermentation...

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饲用酶制剂开发与应用技术交流研讨会

作者： C.Q.Liu,Q.H.Chen,B.Tang,H.Ruan and G.Q.He Department of Food Science and Nutrition,Zhejiang University,Hangzhou 310029,China

Aims:Alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides(RO) that are the factors primarily responsible for flatulence upon ingestion of soybean-derived *** objective of the current work was to develop an optimal culture medium for the production of alpha-galactosidase in solid-state fermentation(SSF) by a mutant strain aspergillus foetidus. Methods and Results:Response surface methodology(RSM) was applied to evaluate the effects of variables,namely the concentrations of wheat bran,soybean meal,KHPO,MnSO·HO and CuSO·5HO on alpha-galactosidase production in the solid substrate.A fractional factorial design(FFD) was firstly used to isolate the main factors that affected the production of alpha-galactosidase and the central composite experimental design(CCD) was then adopted to derive a statistical model for optimizing the composition of the fermentation *** experimental results showed that the optimum fermentation medium for alpha-galactosidase production by aspergillus foetidus ZU-G1 was composed of 8-2137 g wheat bran,1·7843 g soybean meal,0·001 g MnSO·HO and 0·001 g CuSO·5HO in 10 g dry matter fermentation medium. Conclusions:After incubating 96 h in the optimum fermentation medium, alpha-galactosidase activity was predicted to be 2210·76 U g dry matter in 250 ml shake *** the present study,alpha-galactosidase activity reached 2207·19 U g dry matter. Significance and Impact of the Study:Optimization of the solid substrate was a very important measure to increase enzyme activity and realize industrial production of *** process of alpha-galactosidase production in laboratory scale may have the potential to scale-up.

关键词： alpha-galactosidase aspergillus foetidus medium optimization response surface methodology solid-state fermentation

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