检索结果-南通市图书馆

Reversal of tamoxifen resistance by artemisinin in ER+breast cancer:bioinformatics analysis and experimental validation

引用

Oncology Research 2024年第6期32卷 1093-1107页

作者： ZHILI ZHUO DONGNI ZHANG WENPING LU XIAOQING WU YONGJIA CUI WEIXUAN ZHANG MENGFAN ZHANG Department of Oncology China Academy of Chinese Medical Sciences Guang’anmen HospitalBeijingChina

Breast cancer is the leading cause of cancer-related deaths in women worldwide,with Hormone Receptor(HR)+being the predominant subtype.Tamoxifen(TAM)serves as the primary treatment for HR+breast cancer.However,drug resistance often leads to recurrence,underscoring the need to develop new therapies to enhance patient quality of life and reduce recurrence rates.artemisinin(ART)has demonstrated efficacy in inhibiting the growth of drug-resistant cells,positioning art as a viable option for counteracting endocrine resistance.This study explored the interaction between artemisinin and tamoxifen through a combined approach of bioinformatics analysis and experimental validation.Five characterized genes(ar,cdkn1a,erbb2,esr1,hsp90aa1)and seven drug-disease crossover genes(cyp2e1,rorc,mapk10,glp1r,egfr,pgr,mgll)were identified using WGCNA crossover analysis.Subsequent functional enrichment analyses were conducted.Our findings confirm a significant correlation between key cluster gene expression and immune cell infiltration in tamoxifen-resistant and-sensitized patients.scRNA-seq analysis revealed high expression of key cluster genes in epithelial cells,suggesting artemisinin’s specific impact on tumor cells in estrogen receptor(ER)-positive BC tissues.Molecular target docking and in vitro experiments with artemisinin on LCC9 cells demonstrated a reversal effect in reducing migratory and drug resistance of drug-resistant cells by modulating relevant drug resistance genes.These results indicate that artemisinin could potentially reverse tamoxifen resistance in ER-positive breast cancer.

关键词： artemisinin Tamoxifen resistance Breast cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Discovery and repurposing of artemisinin

引用

Frontiers of Medicine 2022年第1期16卷 1-9页

作者： Qiaoli Shi Fei Xia Qixin Wang Fulong Liao Qiuyan Guo Chengchao Xu Jigang Wang artemisinin Research Center and Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Central People’s Hospital of Zhanjiang Zhanjiang 524045China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Department of Geriatrics The Second Clinical Medical College of Jinan UniversityThe First Affiliated Hospital of Southern University of Science and TechnologyShenzhen People’s HospitalShenzhen 518020China

Malaria is an ancient infectious disease that threatens millions of lives globally even today.The discovery of artemisinin,inspired by traditional Chinese medicine(TCM),has brought in a paradigm shift and been recognized as the“best hope for the treatment of malaria”by World Health Organization.With its high potency and low toxicity,the wide use of artemisinin effectively treats the otherwise drug-resistant parasites and helps many countries,including China,to eventually eradicate malaria.Here,we will first review the initial discovery of artemisinin,an extraordinary journey that was in stark contrast with many drugs in western medicine.We will then discuss how artemisinin and its derivatives could be repurposed to treat cancer,inflammation,immunoregulation-related diseases,and COVID-19.Finally,we will discuss the implications of the“artemisinin story”and how that can better guide the development of TCM today.We believe that artemisinin is just a starting point and TCM will play an even bigger role in healthcare in the 21st century.

关键词： artemisinin drug repurposing cancer inflammation COVID-19 traditional Chinese medicine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield

引用

Molecular Plant 2022年第8期15卷 1310-1328页

作者： Baosheng Liao Xiaofeng Shen Li Xiang Shuai Guo Shiyu Chen Ying Meng Yu Liang Dandan Ding Junqi Bai Dong Zhang Tomasz Czechowski Yi Li Hui Yao Tingyu Mai Caroline Howard Chao Sun Haitao Liu Jiushi Liu Jin Pei Jihai Gao Jigang Wang Xiaohui Qiu Zhihai Huang Hongyi Li Ling Yuan Jianhe Wei lan Graham Jiang Xu Boli Zhang Shilin Chen Instituteof ChineseMateria Medica China Academyof ChineseMedical SciencesBeijing 100700China Key Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration the Second Clinical CollegeGuangzhou Universityof ChineseMedicineGuangzhou510006China Institute of Medicinal Plant Development Chinese Academy of Medical SciencesBeijing 100193China artemisinin Research.Center China Academy of Chinese Medical SciencesBeijing 100700China Pharmacy College Chengdu University of Traditional Chinese MedicineChengdu 611137China Centre for Novel Agricultural Products Department of BiologyUniversity of YorkYorkUK Wellcome Sanger Institute Wellcome Trust Genome CampusHinxton CB101RQUK Kentucky Tobacco Research and Development Center University of KentuckyLexingtonKY 40546USA Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement&Guangdong Provincial Key Laboratory of Applied Botany South China Botanical GardenChineseAcademyof SciencesGuangzhou 510520China School of Chinese Materia Medica Tianjin University of Traditional Chinese MedicineTianjin 300193China Chengdu University of Traditional Chinese Medicine Chengdu 611137China

Artemisia annua is the major natural source of artemisinin,an anti-malarial medicine commonly used worldwide.Here,we present chromosome-level haploid maps for two A.annua strains with different artemisinin contents to explore the relationships between genomic organization and artemisinin production.High-fidelity sequencing,optical mapping,and chromatin conformation capture sequencing were used to assemble the heterogeneous and repetitive genome and resolve the haplotypes of A.annua.Approximately 5o,ooo genes were annotated for each haplotype genome,and a triplication event that occurred approximately 58.12million years ago was examined for the first time in this species.A total of 3,903,467-5,193,414 variants(SNPs,indels,and structural variants)were identified in the 1.5-Gb genome during pairwise comparison between haplotypes,consistent with the high heterozygosity of this species.Genomic analyses revealed a correlation between artemisinin concents and the copy number of amorpha-4,11-dienes ynthasegenes.This correlation was further confirmed by resequencing of 36A.annua samples with varied artemisinin contents.Circular consensus sequencing of transcripts facilitated the detection of paralog expression.Collectively,our study provides chromosome-level allele-aware genome assemblies for two A.annua strains and new insights into the biosynthesis of artemisinin and its regulation,which will contribute to conquering malaria worldwide.

关键词： Artemisia annua artemisinin haplotype chromosome amorpha-4 11-diene synthase copy number variation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin levels in Artemisia annua

引用

Journal of Integrative Plant Biology 2022年第6期64卷 1212-1228页

作者： Zongyou Lv JinXing Li Shi Qiu Fei Qi Hang Su Qitao Bu Rui Jiang Kexuan Tang Lei Zhang Wansheng Chen Research and Development Center of Chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Changzheng HospitalSecond Military Medical UniversityShanghai 200003China Department of Pharmaceutical Botany School of PharmacySecond Military Medical UniversityShanghai 200433China Joint International Research Laboratory of Metabolic&Developmental Sciences Key Laboratory of Urban Agriculture(South)Ministry of AgriculturePlant Biotechnology Research CenterFudan‐SJTU‐Nottingham Plant Biotechnology R&D CenterShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China

The important antimalarial drug artemisinin is biosynthesized and stored in Artemisia annua glandular trichomes and the artemisinin content correlates with trichome density;however,the factors affecting trichome development are largely unknown.Here,we demonstrate that the A.annua R2R3 MYB transcription factor TrichomeLess Regulator 1(TLR1)negatively regulates trichome development.In A.annua,TLR1 overexpression lines had 44.7%–64.0%lower trichome density and 11.5%–49.4%lower artemisinin contents and TLR1-RNAi lines had 33%–93.3%higher trichome density and 32.2%–84.0%higher artemisinin contents compared with non-transgenic controls.TLR1 also negatively regulates the expression of anthocyanin biosynthetic pathway genes in A.annua.When heterologously expressed in Arabidopsis thaliana,TLR1 interacts with GLABROUS3a,positive regulator of trichome development,and represses trichome development.Yeast two-hybrid and pull-down assays indicated that TLR1 interacts with the WUSCHEL homeobox(WOX)protein AaWOX1,which interacts with the LEAFY-like transcription factor TLR2.TLR2 overexpression in Arabidopsis and A.annua showed that TLR2 reduces trichome development by reducing gibberellin levels.Furthermore,artemisinin contents were 19%–43%lower in TLR2-overexpressing A.annua plants compared to controls.These data indicate that TLR1 and TLR2 negatively regulate trichome density by lowering gibberellin levels and may enable approaches to enhance artemisinin yields.

关键词： artemisinin Artemisia annua L. LFY MYB transcription factor trichome WOX

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chemical proteomic profiling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin

引用

Chinese Chemical Letters 2023年第12期34卷 486-491页

作者： Peng Gao Jiayun Chen Peng Sun Jianyou Wang Huan Tang Fei Xia Liwei Gu Huimin Zhang Chen Wang Yin Kwan Wong Yinhua Zhu Chengchao Xu Jigang Wang artemisinin Research Center and Institute of Chinese Materia MedicaChina Academy of Chinese Medical Sciences Pharmaceutical College Henan University Shandong Academy of Chinese Medicine Department of Nephrology Shenzhen Key Laboratory of Kidney DiseasesShenzhen Clinical Research Centre for GeriatricsShenzhen People's HospitalThe First Affiliated HospitalSouthern University of Science and Technology

Present research on the antimalarial mechanisms of artemisinin(ART) is mainly focused on covalent drug binding targets alkylated by free radicals, while non-covalent binding targets have rarely been reported.Here, we developed a novel photoaffinity probe of ART to globally capture and identify the antimalarial target proteins of ART through chemical proteomics. The results demonstrated that ART can bind to parasite proteins by both covalent and non-covalent modification, and these may jointly contribute to the antimalarial effects. Our work enriches the research on the antimalarial targets of ART, and provides a new perspective for further exploring the antimalarial mechanism of ART.

关键词： Antimalarial artemisinin Chemical proteomic Photoaffinity probe Target identification

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

artemisinin anti-malarial drugs in China

引用

Acta Pharmaceutica Sinica B 2016年第2期6卷 115-124页

作者： Zongru Guo Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

Discovered by Youyou Tu, one of the 2015 Nobel Prize winners in Physiology or Medicine,together with many other Chinese scientists, artemisinin, artemether and artesunate, as well as other artemisinins, have brought the global anti-malarial treatment to a new era, saving millions of lives all around the world for the past 40 years. The discoveries of artemisinins were carried out beginning from the 1970 s, a special period in China, by hundreds of scientists all together under the "whole nation"system. This article focusing on medicinal chemistry research, briefly introduced the discovery and invention course of the scientists according to the published papers, and highlighted their academic contribution and achievements.

关键词： Antimalarials artemisinin Artemether Artesunate Dihydroartemisinin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

artemisinin and artemisinin derivatives as anti-fibrotic therapeutics

引用

Acta Pharmaceutica Sinica B 2021年第2期11卷 322-339页

作者： David Dolivo Pamela Weathers Tanja Dominko Department of Surgery Northwestern University-Feinberg School of MedicineChicagoIL 60611USA Department of Biology and Biotechnology Worcester Polytechnic InstituteWorcesterMA 01609USA

Fibrosis is a pathological reparative process that can occur in most organs and is responsible for nearly half of deaths in the developed world.Despite considerable research,few therapies have proven effective and been approved clinically for treatment of fibrosis.artemisinin compounds are best known as antimalarial therapeutics,but they also demonstrate antiparasitic,antibacterial,anticancer,and antifibrotic effects.Here we summarize literature describing anti-fibrotic effects of artemisinin compounds in in vivo and in vitro models of tissue fibrosis,and we describe the likely mechanisms by which artemisinin compounds appear to inhibit cellular and tissue processes that lead to fibrosis.To consider alternative routes of administration of artemisinin for treatment of internal organ fibrosis,we also discuss the potential for more direct oral delivery of Artemisia plant material to enhance bioavailability and efficacy of artemisinin compared to administration of purified artemisinin drugs at comparable doses.It is our hope that greater understanding of the broad anti-fibrotic effects of artemisinin drugs will enable and promote their use as therapeutics for treatment of fibrotic diseases.

关键词： Fibrosis Artemisia artemisinin Artesunate Fibroblast Myofibroblast Scar TGF-β

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

artemisinin Biosynthesis in Non-glandular Trichome Cells of Artemisia annua

引用

Molecular Plant 2019年第5期12卷 704-714页

作者： Rika Judd M.Caleb Bagley Mingzhuo Li Yue Zhu Caiyan Lei Seyit Yuzuak Mans Ekelof Gaobin Pu Xiting Zhao David C.Muddiman De-Yu Xie Department of Plant and Microbial Biology North Carolina State UniversityRaleighNCUSA Department of Chemistry North Carolina State UniversityRaleighNCUSA

artemisinin-based combination therapy(ACT)forms the first line of malaria treatment.However,the yield fluctuation of artemisinin has remained an unsolved problem in meeting the global demand for ACT.This problem is mainly caused by the glandular trichome(GT)-specific biosynthesis of artemisinin in all currently used Artemisia annua cultivars.Here,we report that non-GT cells of self-pollinated inbred A.annua plants can express the artemisinin biosynthetic pathway.Gene expression analysis demonstrated the transcription of six known pathway genes in GT-free leaves and calli of inbred A.annua plants.LC-qTOF-MS/MS analysis showed that these two types of GT-free materials produce artemisinin,artemisinic acid,and arteannuin B.Detailed IR-MALDESI image profiling revealed that these three metabolites and dihydroartemisinin are localized in non-GT cells of leaves of inbred A.annua plants.Moreover,we employed all the above approaches to examine artemisinin biosynthesis in the reported XL annua glandless(gl)mutant.The resulting data demonstrated that leaves of regenerated gl plantlets biosynthesize artemisinin.Codectively,these findings not only add new knowledge leading to a revision of the current dogma of artemisinin biosynthesis inannua but also may expedite innovation of novel metabolic engineering approaches for high and stable production of artemisinin in the future.

关键词： Artemisia annua artemisinin glandular trichome norvglandular trichome cell

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

artemisinin as a Chinese Medicine,Selectively Induces Apoptosis in Pancreatic Tumor Cell Line

引用

Chinese Journal of Integrative Medicine 2014年第8期20卷 618-623页

作者： Shokoofe Noori Zuhair.M.Hassan Vida Farsam Department of Biochemistry Faculty of MedicineShahid Beheshti University of Medical SciencesTehran(***)Iran Department of Immunology School of Medical SciencesTarbiat Modares UniversityTehran(***)Iran

Objective： To investigate the possible mechanism through which artemisinin induced apoptosis in pancreatic cell line. Methods： Column chromatography, thin layer chromatography （TLC） and proton NMR spectroscopy were used to purify artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species （ROS）. Results： The results indicated that 50% inhibiting concentration （ICso value） for pancreatic cell line （RIN） was 45 μmol/L of artemisinin. artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intraceliular ROS. It was shown that artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover, artemisinin, in a dose-dependent manner, could significantly increase the level of ROS. Conclusion： artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.

关键词： artemisinin endoperoxide bridge apoptosis reactive oxygen species

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chemical proteomic profling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin

引用

Chinese Chemical Letters 2023年第12期34卷 460-465页

作者： Peng Gao Jiayun Chen Peng Sun Jianyou Wang Huan Tang Fei Xia Liwei Gu Huimin Zhang Chen Wang Yin Kwan Wong Yinhua Zhu Chengchao Xu Jigang Wang artemisinin Research Center and Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Pharmaceutical College Henan UniversityKaifeng 475004China Shandong Academy of Chinese Medicine Ji'nan 250014China Department of Nephrology Shenzhen Key Laboratory of Kidney DiseasesShenzhen Clinical Research Centre for GeriatricsShenzhen People's HospitalThe First Afiliated HospitalSouthern University of Science and TechnologyShenzhen 518020China

Present research on the antimalarial mechanisms of artemisinin(ART)is mainly focused on covalent drug binding targets alkylated by free radicals,while non-covalent binding targets have rarely been reported.Here,we developed a novel photoaffinity probe of ART to globally capture and identify the antimalarial target proteins of ART through chemical proteomics.The results demonstrated that ART can bind to par-asite proteins by both covalent and non-covalent modification,and these may jointly contribute to the antimalarial effects.Our work enriches the research on the antimalarial targets of ART,and provides a new perspective for further exploring the antimalarial mechanism of ART.

关键词： artemisinin Antimalarial Chemical proteomic Target identification Photoaffinity probe

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：