检索结果-南通市图书馆

Comparative cytotoxicity of fourteen trivalent and pentavalent arsenic species determined using real-time cell sensing

引用

Journal of Environmental Sciences 2016年第11期28卷 113-124页

作者： Birget Moe Hanyong Peng Xiufen Lu Baowei Chen Lydia W.L.Chen Stephan Gabos Xing-Fang Li X.Chris Le Division of Analytical and Environmental Toxicology Department of Laboratory Medicine and Pathology Faculty of Medicine and DentistryUniversity of Alberta Alberta Centre for Toxicology Department of Physiology and Pharmacology Faculty of Medicine University of Calgary MOE Key Laboratory of Aquatic Product Safety School of Marine Sciences Sun Yat-Sen University Department of Chemistry Brock University Department of Chemistry and Chemical Biology McMaster University

The occurrence of a large number of diverse arsenic species in the environment and in biological systems makes it important to compare their relative toxicity. The toxicity of arsenic species has been examined in various cell lines using different assays, making comparison difficult. We report real-time cell sensing of two human cell lines to examine the cytotoxicity of fourteen arsenic species： arsenite（As~Ⅲ）, monomethylarsonous acid（MMA~Ⅲ） originating from the oxide and iodide forms, dimethylarsinous acid（DMA~Ⅲ）, dimethylarsinic glutathione（DMAG~Ⅲ）, phenylarsine oxide（PAO~Ⅲ）, arsenate（AsV）, monomethylarsonic acid（MMA~Ⅴ）, dimethylarsinic acid（DMA~Ⅴ）,monomethyltrithioarsonate（MMTTA~Ⅴ）, dimethylmonothioarsinate（DMMTA~Ⅴ）,dimethyldithioarsinate（DMDTA~Ⅴ）, 3-nitro-4-hydroxyphenylarsonic acid（Roxarsone, Rox）,and 4-aminobenzenearsenic acid（p-arsanilic acid, p-ASA）. Cellular responses were measured in real time for 72 hr in human lung（A549） and bladder（T24） cells. IC50 values for the arsenicals were determined continuously over the exposure time, giving rise to IC50 histograms and unique cell response profiles. arsenic accumulation and speciation were analyzed using inductively coupled plasma-mass spectrometry（ICP-MS）. On the basis of the 24-hr IC50 values, the relative cytotoxicity of the tested arsenicals was in the following decreasing order： PAO~Ⅲ？ MMA~Ⅲ≥ DMA~Ⅲ≥ DMAG~Ⅲ≈ DMMTA~Ⅴ≥ As~Ⅲ？ MMTTA~Ⅴ〉 AsV〉 DMDTA~Ⅴ〉DMA~Ⅴ〉 MMA~Ⅴ≥ Rox ≥ p-ASA. Stepwise shapes of cell response profiles for DMA~Ⅲ, DMAG~Ⅲ,and DMMTA~Ⅴcoincided with the conversion of these arsenicals to the less toxic pentavalent DMA~Ⅴ. Dynamic monitoring of real-time cellular responses to fourteen arsenicals provided useful information for comparison of their relative cytotoxicity.

关键词： arsenic species thio-arsenicals methylarsenicals phenylarsenicals toxicity real-time sensing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：