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Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

Synthetic and Systems Biotechnology 2020年第1期5卷 11-18页

作者： Tadas Jakočiūnas Andreas K.Klitgaard Eftychia Eva Kontou Julie Bang Nielsen Emil Thomsen David Romero-Suarez Kai Blin Christopher J.Petzold Jennifer W.Gin Yaojun Tong Charlotte Held Gotfredsen Pep Charusanti Rasmus J.N.Frandsen Tilmann Weber Sang Yup Lee Michael K.Jensen Jay D.Keasling The Novo Nordisk Foundation Center for Biosustainability Technical University of DenmarkKgs.LyngbyDenmark Joint BioEnergy Institute EmeryvilleCAUSA Biological Systems&Engineering Division Lawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Chemical and Biomolecular Engineering&Department of Bioengineering University of California BerkeleyCAUSA Center for Synthetic Biochemistry Institute for Synthetic BiologyShenzhen Institutes for Advanced TechnologiesShenzhenChina Department of Chemistry Technical University of DenmarkKgs.LyngbyDenmark Department of Biotechnology and Biomedicine Technical University of DenmarkKgs.LyngbyDenmark Department of Chemical and Biomolecular Engineering(BK21 Plus Program) Korea Advanced Institute of Science and Technology(KAIST)291 Daehak-roYuseong-guDaejeon34141Republic of Korea

To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms,development of new,versatile,bio-based production platforms is required.Here we present a novel yeast-based platform for biosynthesis of bacterial aromatic polyketides.The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host.

关键词： Natural products Synthetic biology aromatic polyketides CRISPR/Cas9 Polyketide Production platform in eukaryotes Metabolic engineering Biotechnology

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Actinomycetes from the South China Sea sponges:isolation,diversity and potential for aromatic polyketides discovery

Actinomycetes from the South China Sea sponges:isolation,div...

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全国第二届海洋与陆地多糖多肽及天然创新药物研发学术会议

作者： Wei Sun Fengli Zhang Liming He Karthik Loganathan Zhiyong Li Marine Biotechnology Laboratory State Key Laboratory of Microbial MetabolismSchool of Life Sciences and BiotechnologyShanghai Jiao Tong University

Marine sponges often harbor dense and diverse microbial communities including actinobacteria.To date no comprehensive investigation has been performed on the culturable diversity of the actinomycetes associated with South China Sea sponges.Structurally novel aromatic polyketides have been discovered from marine sponge-derived Streptomyces and Saccharopolyspora,suggesting that sponge-associated actinomycetes can serve as an important source of aromatic polyketides.In this study,a total of 77 actinomycete strains were isolated from 15 South China Sea sponge species.Phylogenetic characterization of the isolates based on 16S rRNA gene sequencing supported their assignment to 12families and 20 genera,among which three rare genera(Marihabitans,Polymorphospora and Streptomonospora)were isolated from marine sponges for the first time.Subsequently,β-ketoacyl synthase(KS)gene was used as marker for evaluating the potential of the actinomycete strains to produce aromatic polyketides.As a result,KSgene was detected in 35 isolates related to 7 genera(Kocuria,Micromonospora,Nocardia,Nocardiopsis,Saccharopolyspora,Salinispora and Streptomyces).Finally,ten strains were selected for small-scale fermentation,and one angucycline compound was detected from the culture extract of Streptomycesanulatus strain S71.This study extended our knowledge of the sponge-associated actinomycetes regarding their diversity and potential in producing aromatic polyketides.

关键词： Marine sponges Actinomycetes Diversity Functional gene aromatic polyketides

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