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Sequence Mutation of Exon 7 of Badh2 Gene and aroma identification of Fragrant Rice Varieties

Agricultural Biotechnology 2020年第5期9卷 5-8页

作者： Qingjie TANG Jianxiao CHEN Liqiong TANG Yisheng HAN Xiaowei YAN Cereal Crops Institute Hainan Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Breeding of Hainan ProvinceHaikou 571100China

In this study,we performed amplificaion and sequence analysis of exon7 of gene Badh2 of 12 fragrant rice materials,and identified the aroma of fragrant rice materials by the method of seed chewing and KOH soaking,so as to analyze the sequence mutation of exon 7 in the Badh2 gene of rice material and its corresponding relation with the flavor *** results showed that an 8 bp deletion(aaaa--t---ggc)and a mutation of SNP(g→t)in exon 7 of Badh2 gene were found in 10 materials,including Xiangnuo,Lvjinxiang,Meixiangzhan 2,Huaxiang,Yuexiangxuan 1,Hongyuxiang,Meixiangxuan 1,Baxiangxuan 1,Taixiangxuan 1,Taixiangxuan *** mutation was consistent with the mutation of EU155083 sequence in GenBank and was reported for the first time in Chinese rice *** these 10 fragrant rice materials with mutation,Huaxiang and Meixiangxuan 1 were identified as the heterozygote genotype,and Hongyuxiang was identified as non-fragrant rice,so the sequence mutation in exon 7 of Badh2 gene in fragrant rice materials did not correspond to aroma traits one by one;and 7 materials were identified as fragrant rice,and the brown rice of Meixiangzhan 2 and Yuexiangyuan 1 had sweet *** results could provide a reference for the research on the genetic mechanism of rice aroma character and the promotion of fragrant rice varieties.

关键词： Fragrant rice Badh2 Sequence mutation aroma identification

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