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Quantitative traits correlative analysis and growth comparison among different populations of bay scallop,argopecten irradians

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Acta Oceanologica Sinica 2004年第3期23卷 533-540页

作者： LIUBaozhong LIANGYubo LIUXiaolin DONGBo XIANGJianhai InstituteofOceanology ChineseAcademyofSciencesQingdao266071China NationalMarineEnvironmentMonitoringCenter Dalian116023China CollegeofAnimalScienceandTechnology NorthwestSci-TechUniversityofAgricultureandForestryYangling712100China

The shell traits and weight traits are measured in cultured populations of bay scallop, argopecten irradians. The results of regression analysis show that the regression relationships for all the traits are significant (P0.05). The multiple regression equation is obtained to estimate live body weight and tissue weight. The above traits except anterior and posterior auricle length are used for the growth and production comparison among three cultured populations, Duncan's new multiple range procedure analysis shows that all the traits in the Lingshuiqiao (LSQ) population are much more significant than those of the other two populations (P0.05). The results indicate that the LSQ population has a higher growth rate and is expected to be more productive than the other two populations.

关键词： argopecten irradians quantitative traits correlative analysis growth comparison

Screening microsatellite markers from EST sequences of bay scallop argopecten irradians

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High Technology Letters 2006年第1期12卷 97-102页

作者：王玲玲 Song Linsheng Xu Wei Zhang Huan Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 P.R. China Graduate School Chinese Academy of Sciences Beijing 100039 P.R. China

Seventy-five simple sequence repeats （SSRs） were identified by the bioinformatic analysis from 5008 expressed sequence tags （ESTs） of argopecten irradians. Among the SSRs, the number of repeat nucleotide varied from 2 to 6. Dinucleotide and trinueleotide repeat motifs were dominant in EST-SSRs of bay scallop, with a proportion of 80% over the total screened SSRs. Twenty-nine pairs of primer were designed based on the flank sequences of the selected ESTs using the software of Primet 5, and verified under the given PCR reaction condition. Eighteen of the 29 primer pairs resulted in the expected products, while the remaining either failed to produce any fragments or yielded products over expected size. Thirteen of the 18 SSRs, accounting for 72%, were detected to show polymorphism in the examined scallop samples. A preliminary test in this study indicated that the majority of the identified SSRs were informative in the cultured bay scallops, making them suitable for the population and other genetic analysis. EST-SSR markers have more advantages than the traditional genomic-derived SSRs and there is a wide range of application in comparative mapping, functional gene cloning and marker assisted selection. This research provides a reference to the identification of EST-SSRs with relative bioinformatic analysis from aquaculture species, as well as to those with a large number of ESTs.

关键词： argopecten irradians microsatellite markers expressed sequence tags

CYTOLOGICAL COMPARISON OF GAMETOGENESIS OF SCALLOPS,argopecten irradians AND CHLAMYS FARRERI

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维普期刊数据库

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Chinese Journal of Oceanology and Limnology 2002年第3期20卷 244-247页

作者：董新红刘保忠吴长功相建海 Institute of Oceanology Chinese Academy of Sciences 266071 Qingdao China

Histological characteristics of gametogenesis of two kinds of scallops, gonochoric Chinese scallop, Chlamys farreri and hermaphroditic bay scallop, argopecten irradians were investigated in this study. Spermatogenesis in C. farreri has different developmental stages: spermatogonia, primary spermatocyte, second spermatocyte, spermatid and spermatozoon. A large number of same developmental stage spermatic cells converge at a definite area of the testis. Premeiotic, previtellogenic and vitellogenic oocytes can be found during oogenesis in C. farreri , where oocyte distribution is obviously irregular. The A. irradians gonad consists of two different parts in one individual: one part functions as testis, the other as ovary. Between these two parts is a special appearance area, where a large number of spermatic cells are bound with two layers of acellular substance with many oocytes in it.

关键词： gametogenesis argopecten irradians Chlamys farreri

Effect of E. coli coli on Anti-disease Activities of Scailops: argopecten irradians

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High Technology Letters 2003年第1期9卷 11-16页

作者：胡晓珂 JiangXiaolu LiuShiliang MaiKangsen CollegeofFisheries OceanUniversityofQingdao266003P.R.China ShangdongMarineScienceandTechnologyAcademy Qingdao266071P.R.China

The effect of acute E. coli challenge on the anti-disease activity of scallops argopecten irra-dians is examined. The treatments of scallop from which hemolymph samples were taken for study included (1) control scallops, (2) sham-injected scallops, (3) PSW-injected scallops and (4) E. coli-injected scallops. From the beginning, the anti-disease activities of scallops are deter -mined at 12 hr and 24 hr.The concentrations of circulating hemocytes, the total serum protein concentrations and the activities of alkaline phosphatase, acid phosphatase and superoxide dismutase in the scallops argopecten irradians are determined.Injection with E. coli results in a significant elevation in the concentration of circulating hemocytes and in the alkaline phosphatase activity and a significant decline in the total serum protein concentration and in the superoxide dismutase activity at 24 hr postchallenge. It shows that metabolism of bay scallop is expedited to adopt the challenge.

关键词： argopecten irradians E. coli hemocytes hemolymph alkaline phosphatase acid phosphatase superoxide diamutase mollusk

海湾扇贝(argopecten irradians)两种过氧化物还原酶基因的克隆和结构的研究

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海湾扇贝(Argopecten irradians)两种过氧化物还原酶基因的克隆和...

Cytogenetic analysis in two scallops (Bivalvia:Pectinidae) by PRINS and PI banding

中国动物学会、中国海洋湖沼学会贝类学会分会第十四次学会研讨会

作者：李娟李莉张国范中国科学院海洋研究所国科学院研究生院

过氧化物还原酶(peroxiredoxin,Prx)是一类重要的抗氧化酶,它们广泛存在于原核生物和真核生物体内。这类酶参与生命活动中的氧化还原反应,主要功能是清除机体中的过氧化氢等过氧化物,另外这类酶还具有调节由过氧化氢介导的信号转导和免... 详细信息

过氧化物还原酶(peroxiredoxin,Prx)是一类重要的抗氧化酶,它们广泛存在于原核生物和真核生物体内。这类酶参与生命活动中的氧化还原反应,主要功能是清除机体中的过氧化氢等过氧化物,另外这类酶还具有调节由过氧化氢介导的信号转导和免疫反应的作用。

关键词：海湾扇贝过氧化物还原酶 argopecten irradians Prx RACE

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Acta Oceanologica Sinica 2007年第5期26卷 153-158页

作者： ZHANG Lingling BAO Zhenmin WANG Jue WANG Shi HUANG Xiaoting HU Xiaoli HU Jingjie Laboratory of Marine Genetics and Breeding College of Marine Life Sciences Ocean University of China Qingdao 266003 China

Cytogenetic analysis was performed for the bay scallop （argopecten irradians Lamarck 1819） and the Japanese scallop （Patinopecten yessoensis Jay 1857 ） by primed in situ labeling （PRINS） and propidium iodide （PI） banding techniques. The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A. irradians and on two submetacentric pairs in P. yessoensis. The histone H3 gene sites differed in number and location between these two species. The C-band-like patterns revealed by PI staining varied considerably between these two species. A. irradians displayed terminal bands at long arms on all chromosomes, centromeric bands on some pairs and interstitial bands on five pairs. P. yessoensis exhibited only centromeric bands on all chromosomes. These results would contribute to the better understanding of karyotype evolution in A. irradians and P. yessoensis.

关键词： primed in situ labeling PI banding argopecten irradians Patinopecten yessoensis