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Integrin beta 3-overexpressing mesenchymal stromal cells display enhanced homing and can reduce atherosclerotic plaque

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World Journal of Stem Cells 2023年第9期15卷 931-946页

作者： Hai-Juan Hu Xue-Ru Xiao Tong Li De-Min Liu Xue Geng Mei Han Wei Cui First Division Department of CardiologyThe Second Hospital of Hebei Medical University and Institute of Cardiocerebrovascular Disease of Hebei ProvinceShijiazhuang 050000Hebei ProvinceChina Department of Obstetrics Shijiazhuang People's HospitalShijiazhuang 050030Hebei ProvinceChina Key Laboratory of Medical Biotechnology of Hebei Province Department of Biochemistry and Molecular BiologyCollege of Basic MedicineCardiovascular Medical Science CenterHebei Medical UniversityShijiazhuang 050017Hebei ProvinceChina

BACKGROUND Umbilical cord(UC)mesenchymal stem cell(MSC)transplantation is a potential therapeutic intervention for atherosclerotic vascular *** beta 3(ITGB3)promotes cell migration in several cell ***,whether ITGBmodified MSCs can migrate to plaque sites in vivo and play an anti-atherosclerotic role remains *** To investigate whether ITGB3-overexpressing MSCs(MSCs^(ITGB3))would exhibit improved homing efficacy in *** UC MSCs were isolated and *** vectors encoding ITGB3 or green fluorescent protein(GFP)as control were transfected into *** male apolipoprotein E-/-mice were acquired from Beijing Vital River Lab Animal Technology Co.,Ltd and fed with a high-fat diet(HFD)for 12 wk to induce the formation of atherosclerotic *** HFD-fed mice were randomly separated into three ***-labeled MSCs(MSCs^(GFP))or MSCs^(ITGB3)were transplanted into the mice intravenously via the tail *** staining,Oil red O staining,histological analyses,western blotting,enzymelinked immunosorbent assay,and quantitative real-time polymerase chain reaction were used for the *** ITGB3 modified MSCs successfully differentiated into the“osteocyte”and“adipocyte”phenotypes and were characterized by positive expression(>91.3%)of CD29,CD73,and CD105 and negative expression(<1.35%)of CD34 and Human Leukocyte *** a transwell assay,MSCs^(ITGB3)showed significantly faster migration than ***3 overexpression had no effects on MSC viability,differentiation,and *** staining revealed that ITGB3 overexpression substantially enhanced the homing of MSCs to plaque *** red O staining and histological analyses further confirmed the therapeutic effects of MSCs^(ITGB3),significantly reducing the plaque ***-linked immunosorbent assay and quantitative real-time polymerase chain reaction revealed that MSC^(ITGB3)transplantation considerably decr

关键词： Atherosclerosis Inflammation Integrin beta 3 Mesenchymal stem cells arg-gly-asp structure Umbilical cord

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