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Sensitivity,specificity and predictive values of anterior chamber tap in cases of bacterial endophthalmitis

Eye and Vision 2017年第1期4卷 100-105页

作者： Carl Sjoholm-Gomez de Liano Vidal F.Soberon-Ventura Guillermo Salcedo-Villanueva Abril Santos-Palacios Jose Luis Guerrero-Naranjo Jans Fromow-Guerra Gerardo García-Aguirre Virgilio Morales-Canton Raul Velez-Montoya Retina Department Asociación para Evitar la Ceguera en MexicoHospital“Dr.Luis Sanchez Bulnes”IAPMexico CityMexico Universidad Popular Autonoma del Estado de Puebla PueblaMexico Ophthalmology Department Macula Retina ConsultantsMexico CityMexico

Background:To assess the sensitivity,specificity,positive predictive value and negative predictive value of anterior chamber tap for the diagnosis of bacterial endophthalmitis on a population with high ***:Retrospective,single centre,case series *** reviewed all medical records with clinical diagnosis of bacterial endophthalmitis in our hospital from January 1st,2000 to December 31st *** each record,we documented general demographic data,best corrected visual acuity and vitreous and aqueous tap microbiological *** cases were further divided according to the endophthalmitis aetiology to perform individual calculations of sensitivity,specificity,positive predictive value,negative predictive value,accuracy and *** used the results of the vitreous tap as the gold standard for diagnosis of bacterial *** excluded those records in which the aqueous and vitreous samples were not taken simultaneously or had an incomplete microbiological *** were assessed with chi squared statistics,with an alpha value of 0.05 for statistical ***:A total of 190 cases fulfilled the inclusion/exclusion *** culture rate from vitreous samples was 64.74%.Positive culture rate from aqueous sample was 32.11%.Bacteria isolated from aqueous samples matched those isolated from vitreous samples 78.68%of the *** overall sensitivity was 38.21%,specificity:75.51%,positive predictive value:79.66%,negative predictive value:32.74%(p=0.08).Subgroup analysis showed that anterior chamber taps in cases of post-surgical endophthalmitis had a moderate to low sensitivity(37.73%),high specificity(93%)and high positive predictive value(95%)(p<0.04).Conclusion:The sensitivity and specificity of anterior chamber tap are low and should not be used for critical therapeutic decisions in patients with suspected bacterial *** cases of post-surgical endophthalmitis,the result of an anterior chamber tap

关键词： aqueous humour Endophthalmitis Anterior chamber tap Diagnostic test Vitreous tap aqueous sampling Sensibility Sensitivity

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Effect of oral administration of vitamin C on human aqueous humor ascorbate concentration

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中国药理学报 1999年第10期20卷 879-883页

作者： Zafar IQBAL John M MIDGLEY David G WATSON Stilianous D KARDITSAS Gordon N DUTTON William S WILSON Department of Pharmacy University of StrathclydeGlasgow G11XWUK Tennent Institute of Ophthalmology Western InfirmaryGtasgowG116NTUK Department of Pharmacology University of GlasgowGlasgow G128QQUK

AIM:To study oral administration of vitamin C on human aqueous humour ascorbate ***:High performance liquid chromatography(HPLC)coupled with electrochemical detector(BCD)was *** effect of oral administration of various doses of ascorbic acid,0(control),1.0,1.5,2.0,3.0,and 5.0 g,on its concentration in aqueous humour,obtained from volunteer cataract patients was ***:The concentration of ascorbic acid in aqueous humour of control group without administration of vitamin-C tablet or drug containing ascorbic acid was(254±119)mg·L^(-1).This study revealed that the administration of 2.0 g *** acid saturate the aqueous humour and further increase in the dose(3.0 g and 5.0 g)did not increase its concentration in aqueous humour,although its concentration was increased in ***:Oral administration of 2.0 g of Vc is sufficient to saturate the aqueous humour where it may be helpful in controlling the intra-ocular pressure.

关键词： aqueous humour ascorbic acid plasma cataract extraction high pressure liquid chromatography hydroquinones

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