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Phosphorylation of a wheat Aquaporin at two sites enhances both plant growth and defense

Molecular Plant 2022年第11期15卷 1772-1789页

作者： Kai Lu Xiaochen Chen Xiaohui Yao Yuyan An Xuan Wang Lina Qin Xiaoxu Li Zuodong Wang Shuo Liu Zhimao Sun Liyuan Zhang Lei Chen Baoyan Li Baoyou Liu Weiyang Wang Xinhua Ding Yonghua Yang Meixiang Zhang Shenshen Zou Hansong Dong College of Plant Protection State Key Laboratory of Crop BiologyQilu CollegeShandong Agricultural UniversityTaian 271018China College of Life Sciences Shaanxi Normal UniversityXi’an 710019China Institute of Plant Molecular Biology State Key Laboratory of Pharmaceutical BiotechnologySchool of Life SciencesNanjing UniversityNanjing 210023China nstitute of Plant Protection&Resource and Environment Yantai Academy of Agricultural SciencesYantai 265599China

Eukaryotic Aquaporins share the characteristic of functional multiplicity in transporting distinct substrates and regulating various processes,but the underlying molecular basis for this is largely unknown.Here,we report that the wheat(Triticum aestivum)Aquaporin TaPIP2;10 undergoes phosphorylation to promote photosynthesis and productivity and to confer innate immunity against pathogens and a generalist aphid pest.In response to elevated atmospheric CO_(2)concentrations,TaPIP2;10 is phosphorylated at the serine residue S280 and thereafter transports CO_(2)into wheat cells,resulting in enhanced photosynthesis and increased grain yield.In response to apoplastic H_(2)O_(2) induced by pathogen or insect attacks,TaPIP2;10 is phosphorylated at S121 and this phosphorylated form transports H_(2)O_(2) into the cytoplasm,where H_(2)O_(2)intensifies host defenses,restricting further attacks.Wheat resistance and grain yield could be simultaneously increased by TaPIP2;10 overexpression or by expressing a TaPIP2;10 phosphomimic with aspartic acid substitutions at S121 and S280,thereby improving both crop productivity and immunity.

关键词： Aquaporin phosphorylation gating photosynthesis immunity growth-defense tradeoffs

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Aquaporin,beyond a transporter

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Horticultural Plant Journal 2023年第1期9卷 29-34页

作者： Weikun Jing Yonghong Li Shuai Zhang Xiaofeng Zhou Junping Gao Nan Ma Beijing Key Laboratory of Development and Quality Control of Ornamental Crops Department of Ornamental HorticultureCollege of HorticultureChina Agricultural UniversityBeijing 100193China School of Applied Chemistry and Biological Technology Shenzhen PolytechnicShenzhenGuangdong 518055China Postdoctoral Innovation Practice Base Shenzhen PolytechnicShenzhenGuangdong 518055China

Aquaporins(AQPs)are one of the most ancient superfamily proteins,which are essential for maintaining the fluid homeostasis of most organisms against various environments.Here,the latest findings for function of AQPs in cell signal transduction in plants are summarized.We also put forward several issues that still need to be addressed in the future.

关键词： Aquaporin ABA Signal transduction H_(2)O_(2) Phosphorylation

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Astrocytic Aquaporin 4 subcellular translocation as a therapeutic target for cytotoxic edema in ischemic stroke

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Neural Regeneration Research 2022年第12期17卷 2666-2668页

作者： Adjanie Patabendige Ruoli Chen Department of Biology Edge Hill UniversityOrmskirkUK Brain Barriers Group School of Biomedical Sciences and PharmacyThe University of NewcastleCallaghanNSWAustralia School of Pharmacy and Bioengineering Keele UniversityNewcastle Under LymeStaffordshireUK

Brain edema is a common feature of several brain diseases(e.g.,stroke,traumatic brain injury,hydrocephalus,brain cancer,and brain infections).Brain edema leads to increased intracranial pressure and worsens outcomes in ischemic stroke patients.Conventional treatments to control brain edema,thus reducing intracranial pressure include different osmotherapeutics,hyperventilation,tromethamine,hypothermia,and barbiturate coma.However,level 1 evidence of efficacy is lacking for these treatments,with some being harmful rather than beneficial(Bardutzky and Schwab,2007).It has been proposed Aquaporin 4(AQP4)can be a novel drug target for treating brain edema(Vandebroek and Yasui,2020).

关键词： AQP4 Aquaporin treatment

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The Aquaporin MePIP2;7 improves MeMGT9-mediated Mg2+ acquisition in cassava

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Journal of Integrative Plant Biology 2023年第10期65卷 2349-2367页

作者： Qiuxiang Ma Yancai Feng Shu Luo Lu Cheng Weijing Tong Xinlu Lu Youzhi Li Peng Zhang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/College of Life Science and Technology Guangxi UniversityNanning 530004China

Aquaporins are important transmembrane water transport proteins which transport water and several neutral molecules. However, how Aquaporins are involved in the synergistic transport of Mg2+and water remains poorly understood. Here, we found that the cassava Aquaporin Me PIP2;7 was involved in Mg2+transport through interaction with Me MGT9, a lower affinity magnesium transporter protein. Knockdown of Me PIP2;7 in cassava led to magnesium deficiency in basal mature leaves with chlorosis and necrotic spots on their edges and starch over-accumulation. Mg2+content was significantly decreased in leaves and roots of Me PIP2;7-RNA interference(PIP-Ri) plants grown in both field and Mg2+-free hydroponic solution. Xenopus oocyte injection analysis verified that Me PIP2;7 possessed the ability to transport water only and Me MGT9 was responsible for Mg^(2+)effux.More importantly, Me PIP2;7 improved the transportability of Mg^(2+)via Me MGT9 as verified using the CM66 mutant complementation assay and Xenopus oocytes expressing system. Yeast twohybrid, bimolecular fuorescence complementation,co-localization, and co-immunoprecipitation assays demonstrated the direct protein–protein interaction between Me PIP2;7 and Me MGT9 in vivo. Mg2+fux was significantly elevated in Me PIP2;7-overexpressing lines in hydroponic solution through non-invasive micro-test technique analysis. Under Mg^(2+)-free condition, the retarded growth of PIP-Ri transgenic plants could be recovered with Mg^(2+)supplementation. Taken together, our results demonstrated the synergistic effect of the Me PIP2;7 and Me MGT9 interaction in regulating water and Mg2+absorption and transport in cassava.

关键词： Aquaporin cassava magnesium transporter nutrient utilization protein interaction

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Modulatory effect of Aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells

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Chinese Medical Journal 2021年第4期134卷 448-455页

作者： Yu Fan Tu-Run Song Qiang Wei Lu Yang Tao Lin Xiao-Bing Feng Xian-Ding Wang Zhong-Li Huang Shi-Jian Feng Department of Urology Institute of UrologyOrgan Transplantation CenterWest China Hospital of Sichuan UniversityChengduSichuan 610041China

Background:Estrogen is involved in the pathophysiological process of benign prostatic hyperplasia(BPH),in which epithelial-mesenchymal transition(EMT)plays an important role.Upregulation of Aquaporin(AQP)5,which is directly activated by estrogen,has been reported to promote EMT in multiple cells.This study aimed to examine the effects of AQP5 on estrogen-induced EMT in the prostate.Methods:Normal prostate(NP)tissue samples without any histopathological changes and BPH tissue samples with pathologically confirmed hyperplasia were obtained.An EMT cell model was subsequently established by adding estradiol(E2)to RWPE-1 cells,after which AQP5 knockdown was performed.Tissue morphological and immunohistochemical features were examined using hematoxylin-eosin and immunohistochemical staining.Western blot analysis was performed to determine the expression of AQPs,estrogen receptors,and EMT-related proteins.Cell proliferation was assessed and supernatants were collected for enzyme-linked immunosorbent assay to determine transforming growth factor-β1(TGF-β1)concentrations.Immunofluorescence staining was performed to assess protein expressions in RWPE-1 cells.Results:BPH tissues exhibited greater EMT(TGF-β1:1.362±0.196 vs.0.107±0.067,P=0.003;vimentin:1.581±0.508 vs.0.221±0.047,P<0.001;E-cadherin:0.197±0.188 vs.1.344±0.088,P<0.001),higher AQP5(1.268±0.136 vs.0.227±0.055,P<0.001)and estrogen receptor(ER)α(1.250±0.117 vs.0.329±0.134,P<0.001)expression but lower ERβ(0.271±0.184 vs.1.564±0.130,P<0.001)expression than NP tissues.E2-stimulated cells had higher AQP5 expression(1.298±0.058 vs.1.085±0.104,P=0.049),increased cell proliferation(1.510±0.089 vs.1.000±0.038,P<0.001),and EMT(TGF-β1 concentration:0.352±0.021 ng/mL vs.0.125±0.014 ng/mL,P<0.001;vimentin:1.641±0.120 vs.0.188±0.020,P=0.002;E-cadherin:0.075±0.030 vs.0.843±0.046,P<0.001)than controls.E2-stimulated cells with AQP5 knockdown exhibited decreased EMT(TGF-β1 concentration:0.223±0.041 ng/mL vs.0.352±0.021 ng/mL,P=0.016;vimentin:0.675±0.056 vs.1.641±0.120,P=0.001;E-cadh

关键词： Aquaporin Benign prostatic hyperplasia Epithelial-mesenchymal transition Estrogen Prostate epithelial cells

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Effects of osmotic stress on the expression profiling of Aquaporin genes in the roughskin sculpin(Trachidermus fasciatus)

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Acta Oceanologica Sinica 2020年第6期39卷 19-25页

作者： Qian Ma Xinfu Liu Ang Li Shufang Liu Zhimeng Zhuang College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China

Aquaporins(AQPs)are a family of integral membrane proteins that have been shown to be important for osmoregulation in many vertebrates.To identify potential stress resistance-related aqp genes in salinity adaptation of the roughskin sculpin Trachidermus fasciatus,we investigated the time-course expression dynamics of seven Aquaporin genes(aqp1,4,7,8,10,11 and 12)in three osmoregulatory tissues(kidney,gill and intestine)and one metabolic tissue(liver).The fish were subjected to two different acute osmotic treatments(seawater-tofreshwater transfer respectively achieved in 1 h and 24 h,namely,E-acute and acute group).The expression profiling of the seven aqp genes were performed using quantitative real-time PCR(qRT-PCR).At the time of all sampling time points(0 h,12 h,24 h and 48 h),no expression of aqp4 was found in the gill,liver and intestine;no expression of aqp7 was found in the gill and liver.Significant differences of aqp expression were determined in the four target tissues,and the mRNA levels were largely variable among gene members and tissues.Similar patterns of the time-course expression were detected in most of the aqp genes in T.fasciatus between the two acute groups,except that only one gene(aqp12)in the kidney and three genes(aqp7,aqp8 and aqp10)in the intestine revealed different expression patterns.These results suggest that the expression response of aqp genes was similar under osmotic changes with different rates.

关键词： Trachidermus fasciatus Aquaporin salinity changes stress response gene expression

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Fasudil alleviates LPS-induced lung injury by restoring Aquaporin 5 expression and inhibiting inflammation in lungs

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The Journal of Biomedical Research 2019年第3期33卷 156-163页

作者： Jingjing Wang Hui Kong Jian Xu Yanli Wang Hong Wang Weiping Xie Department of Respiratory & Critical Care Medicine the First Affiliated Hospital of Nanjing Medical University

Fasudil, a selective rho kinase(ROCK) inhibitor, has been reported to play a beneficial role in systemic inflammation in acute lung injury, but its mechanism for ameliorating pulmonary edema and inflammation remains unclear. Using hematoxylin-and-eosin(H&E) staining, immunohistochemistry, enzyme-linked immunosorbent assay,quantitative real time PCR and Western blotting, we found that fasudil attenuated LPS-induced lung injury, decreased lung edema, and suppressed inflammatory responses including leukocyte infiltration and IL-6 production. Further,fasudil upregulated LPS-induced Aquaporin 5 reduction and inhibited NF-κB activation in the lungs of mice. Our results suggest that fasudil could restore the expression of Aquaporin 5 to eliminate LPS-induced lung edema and prevent LPS-induced pulmonary inflammation by blocking the inflammatory pathway. Collectively, blockade of the ROCK pathway by fasudil may be a potential strategy for the treatment of acute lung injury.

关键词： acute lung injury Aquaporin 5 fasudil fliud transport NF-κB

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Genome-wide Association Study Reveals that the Aquaporin NIP1;1 Contributes to Variation in Hydrogen Peroxide Sensitivity in Arabidopsis thaiiana

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Molecular Plant 2017年第8期10卷 1082-1094页

作者： Ayan Sadhukhan Yuriko Kobayashi Yuki Nakano Satoshi luchi Masatomo Kobayashi Lingaraj Sahoo Hiroyuki Koyama Faculty of Applied Biological Sciences Gifu University Gifu 501-1193 Japan Experimental Plant Division RIKEN BioResource Center Tsukuba Ibaraki 305-0074 Japan Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati Guwahati India

Hydrogen peroxide （H202） is a reactive oxygen species that affects cell signaling in various plant defense responses and induces programmed cell death. To identify the new components associated with H202 signaling and tolerance, we conducted a genome-wide association study （GWAS） on the root growth of 133 Arabidopsis thaliana accessions grown in the presence of toxic H2O2 levels. The most significant SNPs were associated with a cluster of chromosome 4 genes encoding an Aquaporin NODULIN 26-LIKE INTRINSIC PROTEIN 1; 1 （NIP1;1）, an NB-ARC domain-containing disease resistance protein （AT4G19050）, and a putative membrane lipoprotein （AT4G19070）. The expression level of NIP1;1 was relatively high in A. thaliana accessions sensitive to H2O2. Additionally, overexpression of NIP1;1 in a tolerant accession （e.g., Col-0） increased the sensitivity of transgenic plants to H2O2. An in planta β-glucuronidase reporter assay revealed that variations in the NIP1;1 promoter were responsible for the differences of its expression level in H2O2-tolerant and -sensitive accessions. Cell death was extensive and H2O2 levels were high in the roots of H2O2-sensitive and NIP1;1-overexpressing accessions. Together, our results indicate that the Aquaporin NIP1;1 is a key determinant of the sensitivity ofA. thaliana to H2O2, and contributes to the phenotypic variations detected by our GWAS.

关键词： Aquaporin GWAS hydrogen peroxide programmed cell death root length signaling

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Interactions between Transmembrane Helices within Monomers of the Aquaporin AtPIP2;1 Play a Crucial Role in Tetramer Formation

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Molecular Plant 2016年第7期9卷 1004-1017页

作者： Yun-Joo Yoo Hyun Kyung Lee Wonhee Han Dae Heon Kim Myoung Hui Lee Jouhyun Jeon Dong Wook Lee Junho Lee Yongjik Lee Juhun Lee Jin Seok Kim Yunje Cho Jin-Kwan Han Inhwan Hwang School of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology Pohang 790-784 Korea Department of Life Sciences Pohang University of Science and Technology Pohang 790-784 Korea Department of Biology Sunchon National University Sunchon 57922 Korea Division of Integrative Biosciences and Biotechnology Pohang University of Science and Technology Pohang 790-784 Korea

Aquaporin （AQP） is a water channel protein found in various subcellular membranes of both prokaryotic and eukaryotic cells. The physiological functions of AQPs have been elucidated in many organisms. However, understanding their biogenesis remains elusive, particularly regarding how they assemble into tetramers. Here, we investigated the amino acid residues involved in the tetramer formation of the Arabidopsis plasma membrane AQP AtPIP2;1 using extensive amino acid substitution mutagenesis. The mutant proteins V41A/ E44A, F51A/L52A, F87A/191A, F92A/193A, V95A/Y96A, and H216A/L217A, harboring alanine substitutions in the transmembrane （TM） helices of AtPIP2;1 polymerized into multiple oligomeric complexes with a vari- able number of subunits greater than four. Moreover, these mutant proteins failed to traffic to the plasma membrane, instead of accumulating in the endoplasmic reticulum （ER）. Structure-based modeling revealed that these residues are largely involved in interactions between TM helices within monomers. These results suggest that inter-TM interactions occurring both within and between monomers play crucial roles in tetramer formation in the AtPIP2;1 complex. Moreover, the assembly of AtPIP2;1 tetramers is critical for their trafficking from the ER to the plasma membrane, as well as water permeability.

关键词： Aquaporin AtPIP2 tetramer formation interaction between transmembrane helices

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龙眼Aquaporin家族的全基因组鉴定及其在体细胞胚发生早期的表达

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应用与环境生物学报 2021年第4期27卷 1001-1012页

作者：赵鹏程徐小萍张春渝陈晓慧申序林玉玲赖钟雄福建农林大学园艺植物生物工程研究所福州350002

水孔蛋白(AQP)是生物调控水跨膜运输的重要通道.为了解龙眼AQP基因家族的生物学功能,利用本实验室的龙眼基因组数据库,结合生物信息学的分析方法进行龙眼全基因组范围内AQP家族成员的鉴定,并对其蛋白质理化性质、系统进化关系、共线性... 详细信息

水孔蛋白(AQP)是生物调控水跨膜运输的重要通道.为了解龙眼AQP基因家族的生物学功能,利用本实验室的龙眼基因组数据库,结合生物信息学的分析方法进行龙眼全基因组范围内AQP家族成员的鉴定,并对其蛋白质理化性质、系统进化关系、共线性、选择压力、基因结构、保守基序、蛋白质保守结构域、顺式作用元件、蛋白质互作和体细胞胚早期不同阶段的基因表达情况进行分析.结果显示,龙眼AQP基因家族共有35个成员,蛋白分子量在17.77×10^(3)-44.30×10^(3) kD之间,分布在14条染色体上;经系统发育树分析,可将其分为5个不同亚家族:PIPs、TIPs、NIPs、SIPs和XIPs;不同亚家族间等电点、磷酸化位点、内含子数量和motif分布差异较大;AQP家族成员间共有6对共线性基因,与拟南芥基因组间有28对共线性基因,且在进化过程中都受纯化选择作用;龙眼AQP家族蛋白质都含有MIP保守结构域;其家族成员可与SYP、PP2A和BOR发生互作,PIP亚家族内的蛋白互作关系最强;基因组的FPKM值分析发现,大部分AQP成员可在龙眼胚胎早期不同阶段进行表达,其中DlTIP4的表达量在所有阶段远高于其他成员,可能在整个过程中发挥着重要作用.本研究表明龙眼35个AQP家族成员基因可能在体细胞胚胎发育阶段发挥重要作用,并且可能响应干旱、低温等非生物胁迫和脱落酸、生长素等植物激素的调控,在功能上具有明显的多样性.

关键词：龙眼 Aquaporin 基因家族全基因组鉴定功能分析

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