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2010全国压电和声波理论及器件技术研讨会

作者： Li-na CHENG~1,Hong-lang LI~1,Yong LIANG~1,Shi-tang HE~1,Zhao-yang TONG~2 1 Institute of Acoustics,Chinese Academy of Sciences,NO.21,Bei-Si-Huan-Xi Road, Beijing,100190,China 2 Beijing Research Institute of Chemical Defence, Beijing,102205,China

A love-wave biosensor has been designed by coupling aptamers to the Au-surface of sensor *** are artificial nucleic acid ligands that can be obtained in the process of *** have advantages over antibodies,such as high affinity,specificity,and *** Love waves' acoustic energy is confined to a thin near-surface region of substrate by guiding layer,Love wave sensors obtain low propagation loss and high *** a result,love wave biosensors using aptamer sensitive layer combining both the advantages stand out in analytical and diagnostic ***,Love-wave sensors are designed as delay-line structure,with a nano-gold sensing area located between the input and output *** lower threshold detection limit,the substrate utilizes ST-90°X quartz,and EWC/SPUDT is used to reduce transducers' bidirectional *** aptamer probe is immobilized by biotin-avidin system(BAS).Depending on the mass multiplied effect of nano-gold particles and biotin-avidin system,the Love wave biosensor can be used for Arbin *** for 1μg/Me Arbin,the system detection is 6.71KHz,which is almost 2 times higher than BSA(BSA concentration is 1.5 mg/Me).The experimental results show that love-wave biosensor can achieve a comparable sensitivity combining with aptamer sensitive layer.

关键词： Love wave aptamer BAS Abrin

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Silver Enhancement-aptamer-Gold Nanoparticles Aggregation Based Assay for Simple and Sensitive Detection of Adenosine

Silver Enhancement-Aptamer-Gold Nanoparticles Aggregation Ba...

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第一届全国生物物理化学会议暨生物物理化学发展战略研讨会

作者： Zhaofang Liu,Jing Ge,and Xin Sheng Zhao~* Beijing National Laboratory for Molecular Sciences,State Key Laboratory for Structural Chemistry of Unstable and Stable Species,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China

＜正＞aptamers are oligonucleic acid or peptide molecules that bind to wide range specific targets from metal ions,small molecules,to large proteins,even *** gold nanoparticles （AuNPs） and silver enhancement were introduced to detect the target molecules[1]. Previous work in our group has prepared aptamer-Gold nanoparticles（Apt-AuNPs） by

关键词： aptamer Gold nanoparticles Adenosine detection Silver enhancement

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Autofluorescence-free targeted tumor imaging based on aptamer-functionalized persistent luminescence nanoparticles

Autofluorescence-free targeted tumor imaging based on aptame...

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第十届全国化学生物学学术会议

作者： Jie Wang Quan Yuan College of Chemistry and Molecular Sciences Wuhan University

Optical bioimaging is an indispensable tool in modern biology and medicine, but the technique is susceptible to autofluorescence interference.1] Persistent luminescence refers to the phenomenon whereby luminescence remains after the cessation of excitation.2] As the lifetimes of biological chromophores are in the range of nanoseconds, tissue autofluorescence can be easily avoided by collecting the persistent luminescence signal after the short-lived autofluorescence has decayed completely.3] However, direct synthesis of persistent luminescence nanoparticles with tunable properties to meet different bioimaging requirements remains largely unexplored.2,3] In this work, zinc gallogermanate(ZnGaGeO:Cr,0≤x≤0.5, ZGGO:Cr) persistent luminescence nanoparticles with composition-dependent size and persistent luminescence are reported. In vivo bioimaging tests demonstrate that ZGGO:Cr nanoparticles can efficiently eliminate tissue autofluorescence. An aptamer-guided ZGGO:Cr bioprobe is constructed and it displays excellent tumor-specific accumulation. The ZGGO:Cr nanoparticles are ideal for autofluorescence-free targeted bioimaging, indicating their great potential in monitoring cellular networks and construction of guiding systems for surgery.

关键词： Bioimaging Autofluorescence aptamer

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In vitro selection and identifcation of DNA aptamer against glucagon receptor by cell-SELEX

In vitro selection and identifcation of DNA aptamer against ...

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华东地区生物化学与分子生物学学会联合会2017年学术交流会暨安徽省生物化学与分子生物学学会理事会议

作者： Guodong Wang Jun Liu Yiling Xu Lei Zhu Xiaoxiao Hu Jianglin Li Wen Zhong Huixia Liu Weihong Tan Anhui Provincial Engineering Research Center for Polysaccharide Drugs School of PharmacyWannan Medical College Xiangya Hospital Central South University Molecular Science and Biomedicine Laboratory State Key Laboratory for Chemo/Bio-Sensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyHunan University

Glucagon,a functional insulin antagonist,is a pivotal counter-regulatory hormone in the regulation of glucose *** secretion of glucagon significantly contributes to *** exerts its physiological functions through activation of the glucagon receptor(GCGR).Inhibition of GCGR activity represents a potential therapeutic approach for reducing excess glucose production in diabetes *** efforts have been undertaken by the pharmaceutical industry to develop potent small molecule glucagon receptor antagonists or antibodies for clinical *** glucagon receptor antagonists and antibodies able to improve glucose homeostasis in animal models and humans have been ***,thus far none has progressed to final marketing approval,mainly due to a poor performance profile,including toxicity or lack of *** are short DNA or RNA oligonucleotides evolved from systematic evolution of ligands by exponential enrichment(SELEX).They can act as ligands with specific and high binding affinity for a variety of targets,including small molecules,proteins,nucleic acids,viruses,bacteria,cells and *** molecular recognition properties of aptamers are similar to those of ***,manmade aptamers possess several advantages over naturally occurring antibodies,including economical and reproducible synthesis,easy modification,low toxicity,high stability,lack of immunogenicity,and rapid tissue *** addition to recognition,some aptamers are able to retain their function to regulate biological pathways and interfere with disease development through binding to molecular targets involved in *** on these advantages,aptamers show high potential for therapeutic applications,such as targeted therapy,detection and *** this study,we have successfully selected a DNA aptamer against GCGR by cell-SELEX after 16 rounds of evolved *** selected aptamer,GR-3,can specifically bi

关键词： glucagon receptor aptamer cell-SELEX diabetes mellitus binding affinity

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Biological Effects of Conformational Alteration Induced by D-/L-Isonucleoside or 2’-Deoxyinosine Incorporation in siRNA or aptamer

Biological Effects of Conformational Alteration Induced by D...

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第八届全国化学生物学学术会议

作者： YANG Zhen-Jun HUANG Ye FAN Xin-Meng CAI Bao-Bin WU Yun ZHANG Li-He State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking University

＜正＞The silencing activities of modified siRNAs（siMekl s,etc）with D-/L-isoNA at several positions of antisense strand dropped dramatically;however,the modification at several po sitions of the sense strand significantly enhanced the silencing activity targeting the antisense strand as reporter and minimized the passenger strand-specific off-target *** investigation of the relationship between the confonnation of modified siRNA or aptamer with IsoNA or 2’-deoxvinosine and their

关键词： siRNA aptamer isonucleoside modification.

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A "turn on" fluorescent nanoprobe for the detection of ATP based on metal–organic frameworks-aptamer

A "turn on" fluorescent nanoprobe for the detection of ATP b...

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河南省化学会2020年学术年会

作者：石鑫鑫孟红敏李朝辉屈凌波郑州大学化学学院

Adenosine triphosphate (ATP),as the source of cellular energy,plays essential roles in the biological processes,such as DNA synthesis and metabolism,and the abnormal ATP level is related to ischemia and some malignant ***,it is of great significance to detect ATP with high sensitivity. aptamer-based fluorescent probes have become the powerful tool to monitor intracellular metal ions and biological ***,most of these probes use carbon materials as quenching agents,such as graphite,graphite oxide and carbon nanotubes,and the preparation of high quality carbon-based materials is still difficult. Metal-organic frameworks (MOFs) constructed using metal ions/clusters and organic linkers have attracted increasing attention in the fields of ***,a rapid,sensitive and accurate design of ATP probe based on MOFs and aptamer is crucial for biochemical *** this work,we designed an aptamer-Cu-MOFs nanoprobe for high sensitive and selective detection of *** uniform Cu-MOFs nanosheets existing conjugatedπ-electron system allows for the binding of single-stranded ATP ***,the FAM-labeled ATP aptamer probe could be adsorbed on the surface of MOF nanosheets,leading to the fluorescence quenching of the FAM through the fluorescence resoncance energy transfer (FRET).However,in the presence of ATP,the FAM-labeled ATP aptamer was is combined with its target ATP,which decreased the the interaction between aptamer and Cu-MOFs ***,the aptamer would detach from the surface of MOFs,which resulted in the fluorescence signal being"turn on".More importantly,the aptamer-Cu-MOFs nanoprobe could be used as a versatile biosensing platform for detection of another biological molecule with high sensitivity and selectivity.

关键词： Metal-organic frameworks ATP aptamer

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An aptamer Based Platform for Capture and Release of Circulating Tumor Cells

An Aptamer Based Platform for Capture and Release of Circula...

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第八届全国化学生物学学术会议

作者： Li Xu Libo Zhao Xiaohong Fang Beijing Natioanl Laboratory for Molecular Sciences Key Laboratory of Molecular Nanostructures and NanotechnologyInstitute of Chemistry Chinese Academy of Sciences

＜正＞Circulating tumor cells（CTC）are rare cancer cells detached from solid tumors and circulated in peripheral *** provided an easy access to solid tumors which can be used for treatment monitoring and tumor molecular *** as"chemical antibody",aptamers have several advantages over commonly used antibody in CTC *** could be used to target specific tumor cells with unknown ***,aptamers are more stable and could

关键词： Circulating tumor cell aptamer Cell-SELEX Microfuidics Nanowires

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Selection of an aptamer against Muscovy duck parvovirus for highly sensitive rapid visual detection by label-free aptasensor

Selection of an aptamer against Muscovy duck parvovirus for ...

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第十三届中国实验动物科学年会

作者：陆涛峰 Qin Ma Wenzhuo Yan Yuanzhi Wang Yuanyuan Zhang Lili Zhao Hongyan Chen 哈尔滨兽医研究所 Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences

Muscovy duck parvovirus(MDPV) causes high mortality and morbidity in ducks. This study investigated a novel aptamer-based, label-free aptasensor detection of MDPV. In this study, we developed an ss DNA aptamer using the filtration partition and lambda exonuclease method with an affinity-based monitor and counter-screening process. After 15 rounds of SELEX(systematic evolution of ligands by exponential enrichment), the ss DNA aptamer Apt-10, which specifically bound to MDPV with high affinity(Kd=467 nM) was successfully screened, and the aptamer was also found to be good specific to MDPV. The selected Apt-10 aptamer can be used to distinguish MDPV and goose parvovirus(GPV). Three-dimensional structural analysis of the Apt-10 aptamer indicated that it folded into a compact stem-loop motif, which was related to its high affinity. Finally, a label-free detection method based on unmodified gold nanoparticles and Apt-10 aptamer was developed for MDPV determination. The concentration of Apt-10 aptamer at 5 μM was optimal for MDPV determination in the label-free aptasensor. Excellent linearity was acquired and the lowest detection limit was 1.5 or 3 EID50(50% egg infection dose) of MDPV, respectively, depending upon spectrophotometry or the naked eye were used. These results show the potential of the aptamer for the rapid detection of MDPV and antiviral research.

关键词： aptamer label-free aptasensor Muscovy duck parvovirus virus detection

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Specific Inhibition of Integrin α3β1-Mediated Tumor Metastasis by a Triple Negative Breast Cancer-Targeted aptamer

Specific Inhibition of Integrin α3β1-Mediated Tumor Metast...

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第十一届全国化学生物学学术会议

作者： Jie Tan Quan Yuan Weihong Tan Molecular Science and Biomedicine Laboratory (MBL) College of Chemistry and Chemical EngineeringHunan University

In the course of cancer cell metastatic, cell adhesion molecules are essential for transducing intracellular signals responsible for adhesion, migration, invasion, and organ-specific metastasis. It has been proved that when the interaction between the metastasis-associated protein and its receptor or ligand is inhibited, the metastasis mediated by this protein will be inhibited as well.[1] aptamers that can specifically bind to proteins and inhibit their activity have already been reported.[2] Herein, we have discovered an aptamer that binds to a line of triple negative breast cancer(TNBC) cells can inhibit the protein-protein interaction of a cell adhesion protein and inhibit cell adhesion. Specifically, by affinity purification, the binding target of this aptamer was identified as tumor metastasis associated protein integrin α3. This aptamer was also found to inhibit the interaction between integrin α3β1 and its ligand protein laminin 10. By cell adhesion assay, the discovered aptamer was demonstrated to inhibit integrin α3β1-mediated adhesion of MDA-MB-231 cells. Therefore, the aptamer obtained here can exhibit great promise to inhibit metastatic activity in preclinical research. In summary, we have examined the function of the anti-integrin α3 aptamer in regulating the tumor cell adhesion. This study is expected to not only demonstrate a new strategy for reduction of tumor metastasis, but also highlight the use of aptamers as novel regulation and inhibition molecules for cellular and molecular interactions.

关键词： protein activity regulation aptamer inhibitor tumor metastatic integrin α3β1

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Computational Simulation of aptamer-target Binding Mechanisms

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中国生物化学与分子生物学报 2024年第11期40卷 1550-1562页

作者： YANG Yuan-Yuan XU Fei WU Xiu-Xiu School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghai Engineering Research Center of Food Rapid DetectionShanghai 200093China

aptamers are a type of single-chain oligonucleotide that can combine with a specific *** to their simple preparation,easy modification,stable structure and reusability,aptamers have been widely applied as biochemical sensors for medicine,food safety and environmental ***,there is little research on aptamer-target binding mechanisms,which limits their application and *** simulation has gained much attention for revealing aptamer-target binding mechanisms at the atomic *** work summarizes the main simulation methods used in the mechanistic analysis of aptamer-target complexes,the characteristics of binding between aptamers and different targets(metal ions,small organic molecules,biomacromolecules,cells,bacteria and viruses),the types of aptamer-target interactions and the factors influencing their *** provides a reference for further use of simulations in understanding aptamer-target binding mechanisms.

关键词： computational simulation aptamer target binding mechanism intermolecular forces

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