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aptamers as Recognition Elements for Electrochemical Detection of Exosomes

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Chemical Research in Chinese Universities 2022年第4期38卷 879-885页

作者： CHANG Kaili SUN Peng DONG Xin ZHU Chunnan LIU Xiaojun ZHENG Dongyun LIU Chao Key Laboratory of Cognitive Science of State Ethnic Affairs Commission Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis and TreatmentCollege of Biomedical EngineeringSouth-Central Minzu UniversityWuhan 430074P.R.China

Exosome analysis is emerging as an attractive noninvasive approach for disease diagnosis and treatment monitoring in the field of liquid *** is considered as a promising molecular probe for exosomes detection because of the high binding affinity,remarkable specificity,and low ***,many approaches have been developed to further improve the performance of electrochemical aptamer based(E-AB)sensors with a lower limit of *** this review,we focus on the development of using aptamer as a specific recognition element for exosomes detection in electrochemical *** first introduce recent advances in evolving aptamers against ***,we review methods of immobilization aptamers on electrode surfaces,followed by a summary of the main strategies of signal ***,we present the insights of the challenges and future directions of E-AB sensors for exosomes analysis.

关键词： aptamer Exosome Biosensor Diagnosis Biomolecular recognition

aptameric SERS sensor for Hg^(2+) analysis using silver nanoparticles

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Chinese Chemical Letters 2009年第12期20卷 1475-1477页

作者： Guo Qing Wang Ling Xin Chen Yantai Institute of Coastal Zone Research for Sustainable Development Chinese Academy of Sciences Yantai 264003 China

aptamer-silver nanoparticles （AgNPs） based surface-enhanced Raman scattering （SERS） sensor has been developed for Hg^2＋ detection by employing the structure-switching aptamer in the presence of spermine. This simple method shows excellent sensitivity and selectivity owing to the sensitive SERS detection technique and high specificity of aptamer for binding Hg^2＋.

关键词： aptamer AgNPs SERS Hg^2+

Artificial Nucleotide-containing aptamers Used in Tumor Therapy

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Chemical Research in Chinese Universities 2020年第2期36卷 164-170页

作者： QIN Xmyuan SU Yuanye TAN Jie YUAN Quan Institute of Chemical Biology and Nanomedicine(ICBN) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyHunan UniversityChangsha 410082P.R.China Key Laboratory of Analytical Chemistry for Biology and Medicine Ministry of EducationCollege of Chemistry and Molecular SciencesWuhan UniversityWuhan 430072P.R.China

The high pharmaceutical cost and multi-drug resistance in tumor therapeutic agents hinder the further application of chemotherapy in tumor *** modified nucleic acid aptamers have the advantages of high binding affinity,programmability,and easy ***,the rational design of artificial modified aptamers is expected to provide a versatile platform for the optimization of chemotherapy *** this review,we summarize the modification strategies and the application of the artificial modified nucleotide-containing aptamers,aiming to provide a promising step toward aptamer-related chemotherapeutic agents.

关键词： aptamer Artificial nucleotide Functional nucleic acid

Recognition mechanism and sequence optimization of organophosphorus pesticides aptamers for better monitoring contaminations in food

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Food Science and Human Wellness 2023年第5期12卷 1708-1715页

作者： Pengfei Chen Chaoqiong Hu Xuan Tao Zheng Zhou Lijun Wang Xiao Yang Zhenming Che Xianggui Chen Yukun Huang Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing School of Food and Biological EngineeringXihua UniversityChengdu 610039China Chengdu Brilliant Pharmaceutical Co. Ltd.Chengdu 610041China Key Laboratory of Food Non-Thermal Processing Engineering Technology Research Center of Food Non-Thermal ProcessingYibinXihua University Research InstituteYibin 644004China

aptamers as a kind of biological recognition element have shown great potential in monitoring and the rapid quantification of organophosphorus pesticides(OPPs). However, molecules of OPPs are structurally similar and original aptamers selected by systematic evolution of ligands by exponential enrichment are usually long-chain bases, which hamper the further application under OPPs-aptamer recognition. The aim of the research was to develop a new strategy to design oligonucleotide sequences for binding OPPs by combination of experimental and molecular modeling methods. 3D models of aptamers binding OPPs were constructed, and binding energy and the most probable binding site for the OPPs were then determined by molecular docking, and the binding sites were further confirmed by the results of 2-AP replaced experiments. Based on the docking results, a new aptamer for detection 4 representative OPPs with only 29 bases was designed by reasonable truncation and mutation of the reported aptamer(named S4-29). The interaction between this new aptamer and OPPs were analyzed by molecular docking, microscale thermophoresis, circular dichroism and fluorometric analysis. The results revealed that the new aptamer exhibit more superior recognition performance to OPPs, which can be promote the monitoring ability of OPPs contaminations in food.

关键词： Organophosphorus pesticides aptamer Recognition mechanism Sequence optimization

In vitro selection of G-rich RNA aptamers that target HIV-1 integrase

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Science China Chemistry 2008年第5期51卷 401-413页

作者： LIU YingChun ZHANG Yan YE GuoZhu YANG ZhenJun ZHANG LiangRen ZHANG LiHe State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100083China

aptamers that interact with various HIV-1 proteins,such as reverse transcriptase,Rev,Tat protein,and nuclear capsule protein,have been prepared through SELEX (systematic evolution of ligands by ex-ponential enrichment) technique. However,there are few reports about the DNA or RNA aptamers that target HIV-1 integrase. In this investigation,we selected alternative RNA aptamers specific for the HIV-1 integrase by using a different binding buffer containing 10 mmol·L-1 MgCl2 and 100 mmol·L-1 KCl. aptamer IN1,IN2,IN3 had similar and the highest Kd values from 145 to 239 nmol·L-1. Structural studies showed that they formed similar stem-loop structure. Deletion of any stem structure resulted in diminished affinity. In addition,structure probing study with antisense DNA indicated that the stem-loop structure in the random region was critical for integrase binding. Although aptamer IN1 failed to form G-quartet structure,it might directly interact with the DDE motif of integrase,which is the virus DNA-binding site,because G-quadruplex T40214 competitively inhibited the interaction between IN1 and integrase. Together,this study generated a novel RNA aptamer IN1,which could be useful in basic research and anti-HIV drug screening.

关键词： RNA aptamer HIV-1 integrase G-quadruplex

Discussion of the protein characterization techniques used in the identification of membrane protein targets corresponding to tumor cell aptamers

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Chinese Chemical Letters 2021年第1期32卷 40-47页

作者： Zhukang Guo Yuan Liu Nongyue He Yan Deng Lian Jin State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China

aptamer is an oligonucleotide chain with specific binding ability to protein and other targets,which is widely used in ma ny *** of its ability to screen the premise of unknown targets,it can be used to discover some novel tumor markers,i.e.,membrane proteins that are specifically highly expressed on the surface of tumor *** markers can be used in many fields such as early diagnosis and treatment,and a new type of tumor marker proved to be effective can significantly improve the therapeutic effect of such ***,further characterization of newly acquired membrane proteins is essential for their clinical use as tumor *** review first briefly introduced the process of obtaining novel tumor markers from nucleic acid ***,the commonly used protein characterization methods could be used as a technical means to identify membrane protein targets corresponding to tumor cell aptamers,to clarify the principles,advantages and disadvantages of various means,and to analyze the most suitable situations for various experimental ***,the outlook was made and the characterization methods that should be used in such experiments were summarized.

关键词： aptamer Cell-SELEX Tumor Membrane protein Tumor marker

Progress in DNA aptamers as Recognition Components for Protein Functional Regulation

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Chemical Research in Chinese Universities 2022年第4期38卷 894-901页

作者： HU Lingling LIU Ke REN Guolan LIANG Jiangong WU Yuan College of Science Huazhong Agricultural UniversityWuhan430070P.R.China

Proteins play a central role in all domains of life,and precise regulation of their activity is essential for understanding the related biological processes and therapeutic *** acid aptamers,the molecular recognition components derived from systematic evolution of ligands by exponential enrichment(SELEX),can specifically identify proteins with antibody-like recognition characteristics and help to regulate their *** minireview covers the SELEX-based selection of protein-binding aptamers,membrane protein analytical techniques based on aptamer-mediated target recognition,aptamer-mediated functional regulation of proteins,including membrane receptors and non-membrane proteins(thrombin as a model),as well as the potential challenges and prospects regarding aptamer-mediated protein manipulation,aiming to supply some useful information for researchers in this field.

关键词： aptamer Protein activity regulation Systematic evolution of ligands by exponential enrichment(SELEX)Membrane receptor Thrombin

Automated screening of primary cell-based aptamers for targeting and therapy of pancreatic cancer

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Chinese Chemical Letters 2024年第2期35卷 300-304页

作者： Zhukang Guo Baijiang Jin Yile Fang Lian Jin Song Li Yan Deng Zhu Chen Hui Chen Yuanying Zhang Rabia Usman Nongyue He State Key Laboratory of Digital Medical Engineering School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China Department of Molecular Biology Jiangsu Cancer HospitalNanjing 210009China

Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased *** address this, primary cells that can be stably passaged were isolated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this ***, an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells, and the obtained nucleic acid aptamers were affinity verified at the cellular ***, to answer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers, we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model, and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.

关键词： aptamer Cell-SELEX Automatic instrument Combination therapy Pancreatic cancer

A DNA micro-complex containing polyaptamer for exosome separation and wound healing

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Chinese Chemical Letters 2024年第9期35卷 257-261页

作者： Jingwen Zhao Jianpu Tang Zhen Cui Limin Liu Dayong Yang Chi Yao Department of Gastroenterology and Hepatology Tianjin Medical University General HospitalTianjin Institute of Digestive DiseaseTianjin Key Laboratory of Digestive DiseasesTianjin 300052China Frontiers Science Center for Synthetic Biology Key Laboratory of Systems Bioengineering(MOE)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China

Exosomes(EXOs)have showed great potential in regenerative *** separation of EXOs from complex biological media is essential for the down-stream ***,we report a deoxyribonucleic acid(DNA)-based micro-complex(DMC)containing polyaptamers,which realized the specific separation of EXOs from cell culture media and the significant promotion of wound *** synthesis of DMCs was based on a biomineralization process via rolling circle amplification(RCA)under the catalysis of phi29 DNA *** endow DMCs with the ability to capture EXOs,the DNA template of RCA was integrated with complementary sequence of aptamer that specifically recognized the CD63 proteins on *** obtained DMCs contained polyaptamers that can specifically capture the EXOs in cell culture *** EXOs-capturing DMCs were collected by centrifugation,achieving the separation of *** stem cell(MSC)-derived EXOs(MSC-EXOs)were separated by this DMC-based strategy,and the separated MSC-EXOs significantly enhanced the migration ability of *** particular,the significant therapeutic efficacy of the DMCs with MSC-EXOs was verified in full-thickness wound excision mouse models,in which the wounds completely healed in 10 *** envision that this DMC-based separation strategy can be a promising route to promote the development of EXOs in biomedicine.

关键词： DNA nanotechnology DNA materials aptamer Exosomes Wound healing