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Pro-apoptotic effects of tectorigenin on human hepatocellular carcinoma HepG2 cells

World Journal of Gastroenterology 2012年第15期18卷 1753-1764页

作者： Chun-Ping Jiang Hui Ding Da-Hua Shi Yu-Rong Wang Er-Guang Li Jun-Hua Wu The First Clinical Medical College NanjingUniversity of Chinese Medicine Nanjing 210046 Jiangsu Province China Department of Hepatobiliary Surgery The Affiliated Drum Tower Hospital Medical School Nanjing University Nanjing 210008 Jiangsu Province China Jiangsu Key Laboratory of Molecular Medicine State Key Laboratory of Pharmaceutical Biotechnology Medical School Nanjing University Nanjing 210093 Jiangsu Province China

AIM:To investigate the effects of tectorigenin on human hepatocellular carcinoma(HCC)HepG2 ***:Tectorigenin,one of the main components of rhizome of Iris tectorum,was prepared by simple methods,such as extraction,filtration,concentration,precipitation and ***2 cells were incubated with tectorigenin at different concentrations,and their viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide *** was detected by morphological observation of nuclear change,agarose gel electrophoresis of DNA ladder,and flow cytometry with Hoechst 33342,Annexin V-EGFP and propidium iodide *** of reactive oxygen species was quantified using *** Ca2+was monitored by Fura *** membrane potential was monitored using Rhodamine *** of cytochrome c from mitochondria to cytosol was detected by Western *** of caspase-3,-8 and-9 were investigated by Caspase Activity Assay ***:The viability of HepG2 cells treated by tectorigenin decreased in a concentration-and timedependent *** concentration that reduced the number of viable HepG2 cells by 50%(IC50)after 12,24 and 48 h of incubation was 35.72 mg/L,21.19 mg/L and 11.06 mg/L,***,treatment with tectorigenin at 20 mg/L resulted in a very slight cytotoxicity to L02 cells after incubation for 12,24 or 48 *** at a concentration of 20 mg/L greatly inhibited the viability of HepG2 cells and induced the condensation of chromatin and fragmentation of *** induced apoptosis of HepG2 cells in a time-and dose-dependent *** with the viability rate,induction of apoptosis was the main mechanism of the anti-proliferation effect of tectorigenin in HepG2 ***,tectorigenininduced apoptosis of HepG2 cells was associated with the generation of reactive oxygen species,increased intracellular[Ca2+]i,loss of mitochondrial membrane potential,transloca

关键词： Tectorigenin Iris tectorum maxim apop-tosis Hepatocellular carcinoma HepG2 Mitochondria Liver cancer

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Neuroprotective effect of Qinggan Lishui formula on retinal ganglion cell apoptosis in a microbead-induced rat chronic glaucoma model

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Journal of Traditional Chinese Medicine 2018年第2期38卷 175-181页

作者： Zhang Cheng Chen Yanqiu Li Fang Wang Zhongfeng Zhang Yinjian Department of Ophthalmology Longhua HospitalShanghai University of Traditional Chinese Medicine Institutes of Brain Science State Key Laboratory of Medical NeurobiologyShanghai Key Laboratory of Visual Impairment and Restoration Collaborative Innovation Center for Brain ScienceFudan University

OBJECTIVE: To investigate a possible mechanism for protective effects of a decoction of the Qinggan Lishui formula(QF) on retinal ganglion cells(RGCs)in a rat model of microbead-induced chronic intraocular hypertension(COH).METHODS: The COH model was generated by injecting microbeads(superparamagnetic iron oxide)into the anterior chamber of rat eyes. QF was given by intragastric administration(gavage) once daily at a dose of 6.2 g/kg until day 28, following microbead injection. Cholera toxin B subunit(CTB) retrograde labeling and immunohistochemistry were used to evaluate changes in the number of RGCs in the retina. Terminal d UTP nick end labeling(TUNEL) staining was used to assess apoptotic changes in ***: Microbead injection induced a steady increase in intraocular pressure(IOP) of rats. Elevated IOP resulted in a progressive reduction in the number of CTB-labeled RGCs, 2-4 weeks after microbead injection. QF administration may moderately reduce IOP in the rat COH model and attenuate reduction of the number of CTB-labeled RGCs in COH rats. Furthermore, elevated IOP resulted in a progressive increase in the number of TUNEL-positive RGCs, 2-4 weeks after microbead injection, suggestive of an increase in the extent of RGC *** was a significant reduction in the number of TUNEL-positive signals in QF-treated COH retinas,compared with untreated COH ***: QF decoction may provide a protective effect for RGCs in COH retinas by reducing RGC loss; these effects may be mediated by inhibition of RGC apoptosis.

关键词： Glaucoma Retinal ganglion cells apop-tosis Qinggan Lishui formula

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