检索结果-南通市图书馆

N-terminal and C-terminal cytosine deaminase domain of APObEC3g inhibit hepatitis b virus replication

World Journal of gastroenterology 2006年第46期12卷 7488-7496页

作者： Yan-Chang Lei Yong-Jun Tian Hong-Hui Ding bao-Ju Wang Yan Yang You-Hua Hao Xi-Ping Zhao Meng-Ji Lu Fei-Li gong Dong-Liang Yang Division of Clinical Immunology Department of Infectious Disease Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China Center of Experimental Medicine Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China Department of Microbiology Tongji Medical College Huazhong University of Science and Technology Wuhan430030 Hubei Province China Institute of Virology Medical School of Duisburg-Essen University Essen Germany Department of Immunology Tongji Medical College Huazhong University of Science and Technology Wuhan430030 Hubei Province China

AIM: To investigate the effect of human apolipoprotein b mrna-editing enzyme catalytic-polypeptide 3g (APObEC3g) and its N-terminal or C-terminal cytosine deaminase domain-mediated antiviral activity against hepatitis b virus (HbV) in vitro and in vivo. METHODS: The mammalian hepatoma cells Hepg2 and HuH7 were cotransfected with APObEC3g and its N-terminal or C-terminal cytosine deaminase domain expression vector and 1.3-fold-overlength HbV DNA as well as the linear monomeric HbV of genotype b and C. For in vivo study, an HbV vector-based mouse model was used in which APObEC3g and its N-terminal or C-terminal cytosine deaminase domain expression vectors were co-delivered with 1.3-fold-overlength HbV DNA via high-volume tail vein injection. Levels of hepatitis b virus surface antigen (HbsAg) and hepatitis b virus e antigen (HbeAg) in the media of the transfected cells and in the sera of mice were determined by *** expression of hepatitis b virus core antigen (HbcAg) in the transfected cells was determined by Western blot analysis. Core-associated HbV DNA was examined by Southern blot analysis. Levels of HbV DNA in the sera of mice as well as HbV core-associated RNA in the liver of mice were determined by quantitative PCR and quantitative RT-PCR analysis, respectively. RESULTS: Human APObEC3g exerted an anti-HbV activity in a dose-dependent manner in Hepg2 cells, and comparable suppressive effects were observed on genotype b and C as that of genotype A. Interestingly, the N-terminal or C-terminal cytosine deaminase domain alone could also inhibit HbV replication in Hepg2 cells as well as Huh7 cells. Consistent with in vitro results, the levels of HbsAg in the sera of mice were dramatically decreased, with more than 50 times decrease in the levels of serum HbV DNA and core-associated RNA in the liver of mice treated with APObEC3g and its N-terminal or C-terminal cytosine deaminase domain as compared to the controls. CONCLUSION: Our findings provide probably the f

关键词： Cytosine deaminase domain apolipoprotein b mrna-editing enzyme catalytic-polypeptide 3g Hepatitis b virus Antiviral therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：