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The Studies on aoz1 gene and its Promoter

The Studies on Aoz1 Gene and its Promoter

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第十一届全国寄生虫学青年工作者学术研讨会

作者： Fugui NIE Xinfang WANG Xiangji YIN Kexin DUAN Yidan QIAO Zehao LI Rui WANG College of Veterinary.Inner Mongolia Agricultural University

The natural enemies of nematodes,the nematode-trapping fungi,have been finally discovered through unremitting efforts of relevant scholars and shown good application prospects for the prevention and control of domestic animal *** years of research by scholars,it has been found that nematode-trapping fungi secrete a variety of extracellular proteases,including serine proteases working during the invasion and digestion of *** order to ascertain more information of the aoz1 gene and its promoter,to further explain the role of in predation of nematode-destroying fungi against parasitic nematodes,the study on the aoz1 gene of Arthrobotrys oligospora and searching for the gene promoter were carried *** research process and results are as follows:(1) Based on the published genome sequence and serine protease gene(aoz1) of Arthrobotrys oligospora,BLAST was used to compare the two sequences to find the aoz1 gene upstream sequence and design primers for amplification and cloning this *** the sequence was analyzed by the promoter online software Promoter Scan,NNPP,Promoter 2.0,and the Cp G island was also predicted with online software EMBOSS and Meth *** results showed that the length of the obtained sequence was 2009 bp,and the homology with the original sequence was 96%;There were TATA box,transcription start site TSS,transcription factor binding site and Cp G island in the sequence of 2009 bp upstream of the start codon of the extracellular serine protease aoz1 of Arthrobotrys oligospora.(2) Recombinant of firefly luciferase reporter gene vector of p GL3-Basic-P1 and 5’-end deletion recombinant vectors,p GL3-Basic-P2 and p GL3-Basic-P3,were constructed and transfected into HEK-293 T cells and He La cells,*** the activity of the luciferase were *** results showed that the 2009 bp upstream of the start codon of the aoz1 gene can significantly increase the expression activity of the firefly luciferase reporte

关键词： aoz1 gene Arthrobotrys oligospora Serine Protease Promoter

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