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Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling

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Horticultural Plant Journal 2024年第2期10卷 532-544页

作者： Muhammad Ahsan Altaf Yuanyuan Hao Huangying Shu Weiheng Jin Chuhao Chen Lin Li Yu Zhang Muhammad Ali Mumtaz Huizhen Fu Shanhan Cheng Guopeng Zhu Zhiwei Wang Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province Sanya Nanfan Research InstituteHainan UniversitySanyaHainan 572025China Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province School of HorticultureHainan UniversityHaikouHainan 570228China Hainan Yazhou Bay Seed Laboratory SanyaHainan 572025China

This study assessed the influence of exogenous ME in the mitigation of cold damage in pepper seedlings. Melatonin(ME) is a dynamic molecule that helps plants cope with stress in several ways. Cold stress(CS) is one of the most important environmental factors that restrict plant growth and yield. Pepper(Capsicum annuum L.) is a valuable commercial crop, highly sensitive to CS. Thus, identifying an efficient strategy to mitigate cold damage is critical for long-term pepper production. For this purpose, the roots of pepper seedlings were pretreated with ME(5 μmol · L^(-1)) and exposed to CS for 7 d. The results indicated that CS suppressed pepper growth, hampered photosynthetic capacity, and damaged root architecture in pepper plants. In contrast, the production of reactive oxygen species(ROS), malondialdehyde(MDA), electrolyte leakage(EL), proline, and soluble sugars were enhanced in plants under CS. ME(5 μmol · L^(-1)) pretreatment reduced the negative effects of CS by recovering plant growth, root traits, gas exchange elements, and pigment molecules compared to CS control treatment. Furthermore, ME application efficiently reduced oxidative stress markers [hydrogen peroxide(H_(2)O_(2)), superoxide ion(O_(2)^(·-)), EL, and MDA] while increasing proline and soluble sugar content in pepper leaves. ME application combined with CS further increased antioxidant enzymes and related gene expression. Collectively, our results confirmed the mitigating potential of ME supplementation for CS by maintaining pepper seedling growth,improving the photosynthesis apparatus, regulating pigments, and osmolyte content.

关键词： Pepper Melatonin Cold stress antioxidant enzyme Root trait

Aβ-Carotene Ketolase Gene NfcrtO from Subaerial Cyanobacteria Confers Drought Tolerance in Rice

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Rice science 2024年第1期31卷 62-76页

作者： GAO Ningning YE Shuifeng ZHANG Yu ZHOU Liguo MA Xiaosong YU Hanxi LI Tianfei HAN Jing LIU Zaochang LUO Lijun College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Shanghai Agrobiological Gene Center Shanghai 201106China College of Life Sciences Shangrao Normal UniversityShangrao 334001China Institute for Agri-food Standards and Testing Technology Shanghai Academy of Agricultural SciencesShanghai 201403China

Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme temperatures.In this study,we identified the drought tolerance gene NfcrtO,which encodes aβ-carotene ketolase,through screening the transcriptome of N.flagelliforme under water loss stress.Prokaryotic expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia coli.When NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on½Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling stage.Transcriptome analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related pathways.Additionally,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H_(2)O_(2))and soil drought treatment at the seedling stage.Physiological traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG treatment.Furthermore,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT line.Yeast two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton pump.Thus,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.

关键词： antioxidant enzyme β-carotene ketolase drought resistance Nostoc flagelliforme osmotic stress rice transcriptome analysis

Effect of powdery mildew on interleaf microbial communities and leaf antioxidant enzyme systems

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林业研究:英文版 2023年第5期34卷 1535-1547页

作者： Xinbo Ma Zhanbin Wang Run Liu Yibing Jiang Northeast Forestry University Harbin 150040People’s Republic of China

Chinese peony(Paeonia lactiflora Pall.)is both medicinally and aesthetically beneficial.Powdery mildew is a common fungal disease that seriously jeopardizes the value of numerous species,including peonies as a crop.In order to provide a basis for the prevention and treatment of peony powdery mildew,we examined the microbial diversity,the malondialdehyde(MDA)concentrations and antioxidant enzyme activities of peony leaves infected with three levels of powdery mildew to determine any modifications to the leaf's antioxidant enzyme systems and microbial community structure following the onset of disease.The results show that the MDA content rose as the degree of infection became worse.antioxidant enzyme activity rose and then declined.Following the initiation of powdery mildew,fungal community diversity decreased,whereas there was not any appreciable change in bacterial communities according to microbial diversity sequencing.The relative abundance of more than half of fungal species decreased,with the bacterial genera displaying both abundant and diminished communities with less pronounced alterations in their community structure after the disease spread.Significant different taxa that were critical to the organization of each microbiome were found.Correlation analysis showed that the relative abundance of powdery mildew pathogenic fungal genus Erysiphe was correlated with those of 11 fungal genera and one bacterial genus.Among them,Aureobasidium,Neosetophoma and Sclerostagonospora showed significant positive correlations with Erysiphe and MDA.

关键词： Powdery mildew Disease level Interleaf microbial diversity antioxidant enzyme

Osmotic Regulation,antioxidant enzyme Activities and Photosynthetic Characteristics of Tree Peony(Paeonia suffruticosa Andr.)in Response to High-Temperature Stress

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Phyton-International Journal of Experimental Botany 2023年第11期92卷 3133-3147页

作者： Zhipeng Sheng Jiasong Meng Jun Tao Daqiu Zhao College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou225009China

Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and development.In this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were investigated.The results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde content.Moreover,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme activities.Also,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature stress.In addition,high-temperature stress led to stomatal closure and chloroplast damage.This study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance.

关键词： Tree peony high-temperature stress antioxidant enzyme photosynthetic characteristics

Effects of excessive nitrogen fertilizer and soil moisture deficiency on antioxidant enzyme system and osmotic adjustment in tomato seedlings

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International Journal of Agricultural and Biological Engineering 2022年第2期15卷 127-134页

作者： Yujie Zhang Shuangen Yu Zijia Li Tingting Chang Qicong Xu Huilian Xu Jie Zhang College of Agricultural Science and Engineering Hohai UniversityNanjing 210098China International Nature Farming Research Center Matsumoto 390-1401Japan

Long-term excessive application of nitrogen fertilizer induces secondary salinization of soil,which results in inhibiting plant growth.In addition,soil moisture deficiency also affects plant growth.To investigate the effects of excessive nitrogen fertilizer and soil moisture deficiency on the antioxidant enzyme system,plant water relations analyzed through pressure-volume(P-V)curve,and photosynthetic light response parameters in tomato(Solanum lycopersicum L.Myoko)seedlings,an indoor experiment of about 50 d was conducted using two irrigation water amounts based on field capacity(soil moisture deficiency:50%-80%;adequate water:70%-80%),two nitrogen fertilizer rates(moderate nitrogen;excessive nitrogen fertilizer:0.585 g/pot)and two types of irrigation water(tap water and microbial diluent).The results showed that excessive nitrogen fertilizer(N)and soil moisture deficiency(W)reduced the biomass of tomato seedlings.In comparison to CK(combination of adequate water and tap water quality),microbial dilution(EM)increased plant biomass by 5.2%.Also,the nitrogen application increased chlorophyll relative contents(SPAD).The maximum net photosynthetic rate(Pc)decreased with nitrogen application and increased with EM application and irrigation amount.Excessive nitrogen application increased the plant nitrate reductase activity(NR).The plant NR in the N treatment showed a 13.0%increase compared to CK,and the plant NR in the treatment of nitrogen application with water deficiency(WN)increased 34.0%compared to water deficiency(W).After applying excessive nitrogen,N,EM-N,WN,EM-WN respectively increased the plant nitrate reductase activity by 13.0%,22.9%,34.0%,and 28.6%,compared with the corresponding treatment with moderate nitrogen(i.e.,CK,EM,W and EM-W).In addition,the activities of antioxidant enzymes[superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)]in four treatments of nitrogen application(N,EM-N,WN,EM-WN)also increased significantly.Both soil moisture and nitrogen fertilizer significantly affect the parameters

关键词： antioxidant enzyme nitrogen osmotic adjustment pressure-volume curve stress tomato water

Effects of Low Temperature Stress on Germination and Physiological Characteristics of Different Sweet Maize Varieties

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Plant Diseases and Pests 2024年第1期15卷 1-3,6页

作者： Zhenxing WU Jianjian CHEN Tingzhen WANG Guihua LU Institute of Maize and Featured Upland Crops Zhejiang Academy of Agricultural SciencesDongyang 322100China

[Objectives]The paper was to explore the effects of low temperature stress on germination and physiological characteristics of different sweet maize varieties.[Methods]Taking Taitian 264,Zhexuetian 1 and Chaotian 4 as the research objects,the changes in germination potential,germination index,plant height,biomass,and antioxidant enzyme activity of maize seeds were studied under optimal temperature conditions(25℃)and low temperature stress conditions(10℃).[Results]Under 10℃stress,the germination rate and germination index of Taitian 264 were higher than that of Zhexuetian 1 and Chaotian 4.Under low temperature stress,Taitian 264 exhibited the least reduction in height and biomass,while Zhexuetian 1 had the most reduction.Additionally,the SOD and POD activities of Taitian 264 were higher than that of Zhexuetian 1 and Chaotian 4 under both temperature conditions,while the MDA content of Taitian 264 was lower.Taitian 264 showed strong germination ability against low temperature stress.[Conclusions]This study provides a basis for timely sowing practices of sweet maize in agricultural production.

关键词： Sweet maize Low temperature stress Germination antioxidant enzyme

Responses of Detoxifying, antioxidant and Digestive enzyme Activities to Host Shift of Bemisia tabaci (Hemiptera: Aleyrodidae)

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Journal of Integrative Agriculture 2013年第2期12卷 296-304页

作者： DENG Pan CHEN Long-jia ZHANG Zong-lei LIN Ke-jian MA Wei-hua Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory Huazhong Agricultural University Institute of Plant Protection Chinese Academy of Agricultural Sciences

The polyphagous B-biotype of Bemisia tabaci （Gennadius） has demonstrated a high capacity to adapt to numerous hosts from diverse plant families. To illustrate induced responses by the host plant, biochemical research on eight plant-insect interaction correlative enzymes, representing detoxifying, antioxidant and digestive pathways, were investigated. Transferring whitefly adults to Zhongmian 23 from the pre-adapted host Zhongmian 41 induced activities of carboxylesterase （by 1.80-fold）, glutathione S-transferase （by 3.79-fold）, proteinase （by 1.62-fold） and amylase （by 2.41-fold） activities, hut decreased polyphenol oxidase （by 1.89-fold） and peroxidase （by 1.88-fold）. However, transferring whitefly adults to the favorite host abutilon from Zhongmian 41 was associated with increased activities of cytochrome P450 monooxygenase （by 1.61-fold） and amylase （by 1.42-fold）, and decreased activities of polyphenol oxidase （by 2.96-fold） and peroxidase （by 2.07-fold）. Our results, together with previous studies, proved that multiple pathways are involved in coping with host shifts by polyphagous herbivores, and the taxonomic status and preference of the host transferred would affect which pathway would be induced. These results would represent a key challenge in developing long-term sustainable insect control strategies.

关键词： Bemisia tabaci biotype B host shift antioxidant enzyme detoxifying enzyme digestive enzyme

Joint effects of Penta-BDE and heavy metals on Daphnia magna survival,its antioxidant enzyme activities and lipid peroxidation

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Frontiers of Environmental Science & Engineering 2011年第1期5卷 99-110页

作者： Baohua TANG Lingyan ZHU Qixing ZHOU Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education) Tianjin Key Laboratory of Environmental Remediation and Pollution ControlCollege of Environmental Science and EngineeringNankai UniversityTianjin 300071China Key Laboratory of Terrestrial Ecological Process Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China

The joint toxicity of Penta-BDE(Pe-BDE)and heavy metals including cadmium and copper on Daphnia magna(D.magna)was evaluated on the basis of determining the 48 h survival,antioxidative enzyme responses,and lipid peroxidation.The response was classified as additive,greater than additive,or less than additive by comparing the measured“toxic units,TU”with one.Based on the survival of D.magna,less-than-additive interactions were found in most of mixtures treatments.This may be attributed to the different toxicity mechanism between Pe-BDE and metals.Cu and Cd played a greater role in toxicity than what Pe-BDE did.As for the superoxide dismutase(SOD)and catalase(CAT)activity,most response was less than additive.For the glutathione S-transferases(GST)activity,most of the greater-thanadditive responses were found in the Cu plus Pe-BDE treatments,but the additive responses occurred in Cd plus Pe-BDE treatments and binary metal treatments.For lipid peroxide levels,which were measured as malondialdehyde(MDA)levels,less-than-additive response occurred in the 50%Cd plus 50%Cu and ternary mixture treatments.Results suggested that Pe-BDE,Cd,and Cu could induce different patterns of antioxidant enzyme responses,such as antioxidant/prooxidant responses,depending on their capability to produce reactive oxygen species and antioxidant enzymes to detoxify them.

关键词： polybrominated diphenyl ethers(PBDEs) heavy metal mixture toxicity toxic units(TUs) antioxidant enzyme lipid peroxidation

Effects of cold-hardening on compatible solutes and antioxidant enzyme activities related to freezing tolerance in Ammopiptanthus mongolicus seedlings

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Forestry Studies in China 2008年第2期10卷 101-106页

作者： WANG Wei-juan CHEN Yu-zhen LIU Mei-qin LU Cun-fu College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China

Cold acclimation is associated with many metabolic changes that lead to an increase of freezing tolerance. In order to investigate the biochemical process of cold acclimation in Ammopiptanthus mongolicus, seedlings were acclimated at 2℃ under 16-h photoperiod （150 μmol·m^-2·s^-1 photosynthetically active radiation） for 14 d. Freezing tolerance in seedlings increased after 14 d of cold-hardening. Contents of protein, proline and solute carbohydrate in cotyledon increased after cold acclimation. Patterns of isozymes of superoxide dismutase （SOD）, peroxidase, catalase and polyphenol oxidase （PPO） were investigated. The activities of SOD, peroxidase and PPO in cold acclimated plants were increased during cold-hardening. We deduced that compatible solutes and antioxidant enzymes play important roles in development of freezing tolerance during cold acclimation in this evergreen woody plant.

关键词： Ammopiptanthus mongolicus cold acclimation freezing tolerance compatible solute antioxidant enzyme