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Microwave Assisted Extraction,antioxidant potential and Chromatographic Studies of Some Rasayana Drugs

Chinese Journal of Integrative Medicine 2015年第7期21卷 523-529页

作者： Ashish Mishra Shilpi Mishra Shilpi Bhargav CS.Bhargava Mayank Thakur Advance Institute of Biotech&Paramedical Sciences Institute for Laboratory Medicine Clinical Chemistry and Pathobiochemistry Charite University of MedicineCampus Benjamin Franklin

Objective： To study and compare the conventional extraction procedure with microwave assisted extraction （MAE） for some Ayurvedic Rasayana drugs and to evaluate their antioxidant potential and carry out the characterization of extracts by thin layer chromatography. Methods： Three Ayurvedic rasayana plants Allium sativum Linn., Bombax ceiba Linn. and Inula racemosa Hook. were evaluated for an improved MAE methodology by determining the effects of grinding degree, extraction solvent, effect of dielectric constant and duration of time on the extractive value. antioxidant potential of all three drugs was evaluated with 2,2-diphenyl-l-picrylhydrazyl （DPPH） radical scavenging activity and reducing power was determined by using Gallic acid as standard. Further thin layer chromatographic （TLC） analysis was performed on pre-activated Silica Gel G plates and Rf value were compared with those reported for the important biomarkers. Results： The total extractive value for Allium sativum Linn. was 36.95% （w/w） and 49.95% （w/w） for ethanol extraction respectively. In case of Bombax ceiba Linn. the yield of aqueous extract by MAE was 50% （w/w） compared to 42% （w/w） in ethanol （50% v/v）. Percent yield of Inula racernosa Hook. in aqueous extract was found to be 27.55% （w/w） which was better than ethanol extract （50%） where the yield was 25.95% （w/w）. Upon antioxidant activity evaluation A. safivum extract showed an absorbance of 0.980 ± 0.92 at concentration of 500 μg with maximum reducing capacity. This was followed by B. ceiba Linn. 0.825 ± 0.98 and I. racemosa Hook. with 0.799 ± 2.01 at a concentration of 500 μ g. TLC based standardization of A. sativum Linn. extract shows single spot with Rf value of 0.38, B. ceiba Linn. extract shows Rf values were 0.23, 0.58, 0.77, 0.92 and I. racemosa Hook. extract spot had a Rf value of 0.72. Conclusions： A significant improvement in extractive values was observed as a factor of time and other advantages by using MAE technology. All three dru

关键词： antioxidant potential Ayurvedic Rasayana Allinm sativum Linn. Bombax ceiba Inula racemosa,microwave assisted extraction

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Impact of Extraction on Biochemical Properties and antioxidant potential of Momordica charantia L. Seeds’ Oil

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Food and Nutrition Sciences 2022年第2期13卷 147-164页

作者： Balde Samba Ayessou Nicolas Cyrille Khadim Niane Bou Ndiaye Mady Cisse Codou Mar Diop Centre d’Etude sur la Sécurité Alimentaire et les Molécules fonctionnelles (CESAM) Université Cheikh Anta DIOP de Dakar Fann Sénégal

In this study, the influence of provenance and extraction methods on the physicochemical properties and the antioxidant potential of M. charantia seeds oil were evaluated. The oil is obtained on the one hand by cold extraction with hexane and on the other hand by hot extraction with soxhlet. The results obtained show that the extraction yield is significantly impacted by the extraction methods and the origin of the seeds. In addition, the soxhlet extraction gives a higher oil yield (32.07 ± 0.01). Cold extraction has made it possible to obtain oils with less attenuated physicochemical characteristics. Indeed, the acid numbers are high in the oils extracted by soxhlet (5.92 ± 0.25;4.25 ± 0.62 and 13.86 ± 0.83) than in those cold extracted with very low peroxide for all the oils obtained. On the other hand, the iodine and refractive indices are high in oils obtained cold (91.58 ± 0.85;100.74 ± 0.03 and 102.08 ± 0.28) (1.53 ± 0.01;1.52 ± 0.01 and 1.52 ± 0.01) with low saponification indices. The polyphenol concentrations and the anti-free radical activity are higher with the oils obtained cold (0.086 ± 0.001;0.08 ± 0.000 and 0.09 ± 0.01 mgEAG/g of oil) and (DPPH) (55.75% ± 1.16%;55.03% ± 0.72% and 56.35% ± 0.45%). The color parameters (L*, a* and b*) of the different oils extracted also vary depending on the extraction method used. Principal Component Analysis (PCA) and correlation analysis were performed on the physicochemical properties and the antioxidant potential of the extracted oils. Therefore, the results suggest cold extraction to obtain a good quality and oxidation resistant oil.

关键词： Momordica charantia Oil antioxidant potential Extraction

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In vitro antioxidant,anti-diabetic,cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and *** fruits

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Food Science and Human Wellness 2014年第1期3卷 16-25页

作者： Arumugam Abirami Gunasekaran Nagarani Perumal Siddhuraju Bioresource Technology Lab School of Life SciencesDepartment of Environmental SciencesBharathiar UniversityCoimbatore 641046Tamil NaduIndia

In the present study,antioxidant potential,α-amylase,α-glucosidase,cholinesterase and tyrosinase inhibitory activity of fresh juice from indigenous fruits of Citrus hystrix and ***(Red&White var.)were investigated using an in vitro *** contents of total phenolics,tannins,and total flavonoids ranged between 836.90 and 909.52 mg gallic acid equivalent(GAE)/L,333.33 and 523.21 mg gallic acid equivalent(GAE)/L and 224.88 and 262.22 mg rutin equivalent/L,*** antioxidant activity of fresh juice was evaluated by employing different in vitro assays such as reducing power assay,DPPH•,ABTS•+and•OH radical scavenging capacities,peroxidation inhibition activity,antihemolytic *** addition,75.55%–79.75%ofα-amylase and 70.68%–72.83%ofα-glucosidase enzyme inhibition characteristics were found under in vitro starch digestion ***,all the juice samples exhibited excellent tyrosinase(76.95%–80.79%),acetylcholinesterase(75.71%–79.74%)andβ-glucuronidase inhibitory activity(68.13%–69.38%).These results indicated that fresh juice of *** and ***(Red&White var.)could be used as a source of antioxidant agents,functional food and nutraceuticals.©2014 Beijing Academy of Food *** and hosting by Elsevier *** rights reserved.

关键词： Citrus fruits Fresh juice antioxidant potential Enzymatic inhibitory activity Nutraceuticals

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Identification of novel bioactive proanthocyanidins with potent antioxidant and anti-proliferative activities from kiwifruit leaves

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Food Bioscience 2022年第2期46卷 539-549页

作者： Ji-Min Lv Mostafa Gouda Alaa El-Din Bekhit Ya-Ke He Xing-Qian Ye Jian-Chu Chen College of Biosystems Engineering and Food Science National-Local Joint Engineering Laboratory of Intelligent Food Technology and EquipmentZhejiang Key Laboratory for Agro-Food ProcessingZhejiang Engineering Laboratory of Food Technology and EquipmentZhejiang UniversityHangzhou310058China Department of Nutrition and Food Science National Research CentreDokki12622GizaEgypt Department of Food Science University of OtagoOtagoNew Zealand

Agro-wastes contribute major social,economic,and environmental challenges for food production and circular economy *** current increasing demand for clean label food production and use of natural bioactive compounds could turn these challenges into opportunities providing avenues for proper utilization of agro-wastes to produce valuable *** study aimed to investigate the potential use of kiwifruit(Actinidia chinensis)leaves as a source of proanthocyanidins(PAs)bioactive phenolic *** leaves PAs were extracted,purified,identified,and evaluated for their antioxidant and anti-proliferative *** structural composition of the purified PAs was characterized using HPLC-QTOF-MS/MS and *** results showed that purified kiwifruit leaves PAs(PKLPs)comprised mainly procyanidins,propelargonidins,and prodelphindins ranging from dimers to hexamers with(epi)catechin as terminal units and(epi)afzelechin or(epi)gallocatechin as dominant extension *** study reports the structure of novel PKLPs monomer fractions was unique compared to the PAs that extracted from the other plant *** PKLPs exhibited higher phenolic content than the skin and flesh of several kiwifruit ***,the PKLPs exhibited higher in vitro antioxidant activity in chemical-based(DPPH,ABTS,and FRAP)assays and H2O2-induced injury cell model than ascorbic,Trolox,and catechin(p<0.01).A remarkable dose-dependent anti-proliferation activity(IC_(50)=186.04±2.61μg/mL)against HepG2 cells was *** conclusion,this study demonstrated that kiwifruit leaves waste could serve as a sustainable and low-cost source of PAs,a group of multi-functional bioactive compounds that plays a key role in the food and pharmaceutical industries.

关键词： Proanthocyanidins Agro-waste valorisation HPLC-QTOF-MS/MS antioxidant potential Anti-proliferative activity

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Techno and bio functional characterization of industrial potato waste for formulation of phytonutrients rich snack product

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Food Bioscience 2022年第5期49卷 1061-1071页

作者： Lovepreet Singh Sukhpreet Kaur Poonam Aggarwal Department of Food Science and Technology Punjab Agricultural UniversityLudhiana141004PunjabIndia

Present study aimed at utilizing potato industry waste(potato peel and mash)to improve the phytonutritional profile of chickpea flour-based snack product *** waste was converted into powders and studied for their respective biochemical and functional *** of potato peel powder showed high crude fiber(13.05%),protein(13.15%),and bio-functional properties(834.24 mg GAE/g total phenols and 247.18 mg QE/mg total flavonoids)while mash powder exhibited high techno-functional potential as water(4.11 g/g)and oil absorption capacity(1.70 g/g).Mash and peel powders were incorporated in chickpea flour at different concentrations for formulation of *** to control,addition of these powders considerably(pantioxidant activity was observed in stored *** to high antioxidant potential of potato peels,lower lipid oxidation values were noticed for potato waste enriched snack throughout the storage and the product was shelf stable at room temperature for six months without impacting *** utilization of potato waste in prepared product can provide dual benefit of producing healthy phytonutrients rich snack product along with solving the problem of solid waste dispersal of potato processing industry.

关键词： Potato peel Functional properties Phytonutrients antioxidant potential HPLC

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Evaluation of potential Extracts Antioxydant (Aqueous, Hydro-Ethanolic and Ethanolic) of an Aquatic Plant from the River Djoue (<i>Ledermanniella schlechteri</i>)

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Open Journal of Applied Sciences 2021年第3期11卷 254-263页

作者： Roniche Nguie Tsiba Gouollaly Arnaud W. G. Tamba Sompila J. E. Moussounga M. Gadet Dzondo N. P. G. Pambou-Tobi P. Diakabana Reyes H. Gampoula Centre de Recherche et d’Initiation des Projets de Technologie cité scientifique route de l’auberge de Gascogne Brazzaville Congo EPRAN-Congo P&#244 le d’Excellence en Alimentation et Nutrition Faculté des Sciences et Techniques Université Marien NGOUABI Brazzaville Congo Institut national de Recherche en Sciences de la Santé cité scientifique route de l’auberge de Gascogne Brazzaville Congo Unité de Chimie du Végétal et de la Vie FST-UMNG Brazzaville Congo Laboratoire de Bioprocédés alimentaires et médicaux ENSP-UMNG Brazzaville Congo Ecole Nationale Supérieure Polytechnique (ENSP) Université Marien NGOUABI (UMNG) Brazzaville Congo Ecole Supérieure de Technologie des Cataractes Brazzaville Congo

The aim for this present study was to evaluate the antioxidant potential of aqueous extracts (AE), hydro-ethanolic extracts (HE) and ethanolic extracts (EE) obtained from an aquatic plant (L. schlechteri) using a simple and fast method that is the CCM. This method revealed the presence of phenolics and flavonoids at different levels but with higher antioxidant activity in EE compared to AE. Among the two families of antioxidants evaluated, the phenolic compounds were found to be higher on the EE (5.85 mgEAG/MS) followed by the HE (5.06 mgEAG/MS) and less and less important on the AE (3.661 mgEAG/MS). While the less significantly elevated flavonoids showed values of 1.146 mgECa/MS for EE, 0.406 mgECa/MS for HE et 0.181 mgECa/MS for AE. However, the anti-free radical activity was also evaluated. Unlike the antioxidant activity, the ant-free radical activity with a greater IC50 was observed on AE with a rate of 66.66 mg/mL, then less on the hydro-ethanolic and ethanolic extracts, respectively at levels of 26.15 mg/mL et 19.18 mg/mL.

关键词： Evaluation antioxidant potential Extracts Aquatic Plant Ledermanniella schlechteri

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Chios mastic treatment of patients with active Crohn's disease

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World Journal of Gastroenterology 2007年第5期13卷 748-753页

作者： Andriana C Kaliora Maria G Stathopoulou John K Triantafillidis George VZ Dedoussis Nikolaos K Andrikopoulos Department of Science of Dietetics-Nutrition Harokopio University Athens Greece Department of Gastroenterology Saint Panteleimon General State Hospital Nicea Athens Greece

AIM: To evaluate the effectiveness of mastic administra-tion on the clinical course and plasma inflammatory me-diators of patients with active Crohn’s disease (CD).METHODS: This pilot study was conducted in patients with established mild to moderately active CD, attend-ing the outpatient clinics of the hospital, and in healthy controls. Ten patients and 8 controls were recruited for a 4-wk treatment with mastic caps (6 caps/d, 0.37 g/cap). All patients successfully completed the protocol. CD Ac-tivity Index (CDAI), Nutritional Risk Index (NRI), C-re-active protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), monocyte chemotactic protein-1 (MCP-1), and total antioxidant potential (TAP) were evaluated in the plasma at baseline and at the end of the treatment period. Results were expressed as mean values ± SE and P < 0.05 was considered to indicate statistical ***: Patients exhibited significant reduction of CDAI (222.9 ± 18.7 vs 136.3 ± 12.3, P = 0.05) as com-pared to pretreament values. Plasma IL-6 was signifi-cantly decreased (21.2 ± 9.3 pg/mL vs 7.2 ± 2.8 pg/ mL, P = 0.027), and so did CRP (40.3 ± 13.1 mg/mL vs 19.7 ± 5.5, P = 0.028). TAP was significantly increased (0.15 ± 0.09 vs 0.57 ± 0.15 mmol/L uric acid, P = 0.036). No patient or control exhibited any kind of side effects. CONCLUSION: The results suggest that mastic signifi-cantly decreased the activity index and the plasma levels of IL-6 and CRP in patients with mildly to moderately ac-tive CD. Further double-blind, placebo-controlled studies in a larger number of patients are required to clarify the role of this natural product in the treatment of patients with CD.

关键词： Chios mastic Crohn's disease C-reactive protein Cytokines antioxidant potential Conservativetreatment

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Phytochemicals Analysis, antioxidant Capacities and Antimicrobial Properties of Ethyl Acetate Extract of Stem Bark of the Garlic Tree Scorodocarpus borneensis Becc.

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Journal of Agricultural Science and Technology(A) 2016年第6期6卷 403-410页

作者： Sudrajat Sudrajat Dwi Susanto Sudiastuti Sudiastuti Department of Biology Faculty of Mathematics and Natural Sciences Mulawarman University Jalan Barong Tongkok No. 4Samarinda 75123 East Kalimantan Indonesia

The objective of this study was to examine the phytochemical components, antioxidant activity and antibacterial property of ethyl acetate extract of the stem bark of garlic tree （Scorodocarpus borneensis）. The dried stem bark of S. borneensis were collected and homogenized after drying at room temperature （32℃） for 30 d. The stem barks were extracted by macerated method using 95% ethanol and then fractionated with ethyl acetate. The dried ethyl acetate extract was subjected to phytoehemical screening to determine the presence of bioactive components using gas chromatography-mass spectrometry （GC-MS）. antioxidant activity of the extract in vitro was examined by 2,2-diphenyl-l-picryl-hydrazyl （DPPH） radical scavenging assay. The antibacterial activity against gram-positive bacteria Staphylococcus aureus and gram-negative bacteria Escherichia coli was performed by disc diffusion assay. GCMS results revealed the presence of 14 different phytocompounds, viz, tetratriacontyl trifluoroacetate （41.61%）, 2-pentanone （13.65%）, oxacyclotetradecane-2,11-done （7.87%）, cinnamic acid （7.53%）, 10-octadecanoic acid （6.50%）, 1,2-benzeno dicarboxylix acid （4.99%）, octadecanoic acid （4.51%）, hexadecanoic acid （4.16%）, beta tumerone （3.01%）, 9-octadecenoic acid （1.70%）, tricosanol （1.38%）, hexadecano-phenone （1.36%）, 1-nonadecanol （0.93%） and n-nonadecanol （0.82%）. In vitro antioxidant activity （IC50） was found at 55.524 ppm as high powerful. The results of agar diffusion method showed that the ethyl acetate extracts had an antibacterial activity of 6.687 ± 0.800 mm againts S. aureus at 10% （w/v） and 7.500 ± 0.735 mm against E. coli at 10% （w/v） as moderate category. These findings suggest that S. borneensis stem bark is a valuable sources of bioactive compounds with promising as antioxidant and antibacterial sources.

关键词： Stem bark ofScorodocarpus borneensis phytochemicals antioxidant potential antimicrobial properties.

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Protein and Phenolic Contents and antioxidant Activities of 14 Early Maturing Potatoes as Affected by Processing

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American Journal of Plant Sciences 2016年第1期7卷 69-81页

作者： Reena Grittle Pinhero Rong Tsao Qiang Liu James Alan Sullivan Benoit Bizimungu Rickey Y. Yada Department of Food Science University of Guelph Guelph Canada Agriculture and Agri-Food Canada Guelph Food Research Centre Guelph Canada Department of Plant Agriculture University of Guelph Guelph Canada Agriculture and Agri-Food Canada Fredericton Canada Faculty of Land and Food Systems University of British Columbia Vancouver Canada

The effects of processing and genotype (fourteen early potato varieties) were evaluated for their phytochemicals, total phenolics (TPC), total flavonoids (TFC), total anthocyanins (TAC), antioxidant activities measured by ORAC, FRAP and DPPH assays and protein content. While all these profiles were highly dependent on the potato variety and processing, F09085, F10090, French Fingerlings, Purple Fiesta, Red Thumb, Ciklamen and Norland were identified as nutritionally rich in phytochemicals such as TPC, TFC, TAC, higher antioxidant capacities and protein (p p p < 0.0001).

关键词： Potato Phenolic Content Anthocyanins antioxidant potential Protein

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